查询词典 mammary feminism

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Methods:The level of E2 was evaluated by Progesrone Serozyme,the expression of ER was tested by Immunohistocheminal assay,ER gene expression was tested by RT-PCR in 35 cases with mammary hyperplasia of stagnation of Liver-qi type,15 cases with mammary hyperplasia of disorder of thoroughfare vessel and conception Vessel type and 30 cases of healthy people were taken as control group.

分别用磁分离酶联免疫测定法、免疫组化法和逆转录—聚合酶链反应法，检测35例肝郁气滞型乳腺增生病患者及15例冲任失调型患者和30例正常人黄体期血清雌激素水平（E2 ）、乳腺组织雌激素受体及受体基因的表达。

Indications: JiaJian hyperthyroidism, subjects, hashimoto's thyroiditis, JiaYan, parathyroid function lowly disease of mammary gland hyperplasia of mammary gland fibroma,,, prostatitis, hyperplasia, seminal vesicle phlogistic, liver cavernous hemangioma, thromboangiitis obliterans, Renault's disease, erythema limb pains, eczema, ectopic dermatitis, nodular psoriasis skin erythema, etc.

主治科目：甲亢、甲减、桥本氏甲状腺炎、亚甲炎、甲状旁腺机能低下症、乳腺纤维瘤、乳腺增生、前列腺炎、前列腺增生症、精囊炎、肝海绵状血管瘤、血栓闭塞性脉管炎、雷诺氏病、红斑肢痛症、湿疹、异位性皮炎、结节性红斑、银屑病等皮肤病。

For the purpose of constructing mammary gland specific expressional vector, we use the updream regulation sequence of bovine BLG to regulate lacS gene to express in mammary gland. Positive clone cells are selected and purified by the expression Neo gene and report gene EGFP. So the cells can be used as sources to create transgenic animals by nuclear transfer techniques.

本研究以牛β-乳球蛋白基因上游调控成分作为启动子，嗜热菌糖苷酶基因lacS为目的基因，构建了lacS基因乳腺特异表达载体，并通过脂质体介导法转染体外培养的牛胎儿成纤维细胞，借助新霉素抗性基因和增强型绿色荧光蛋白报告基因来筛选、纯化阳性克隆细胞，为利用核移植技术制备转基因动物提供细胞来源。

Objective: To investigate the research approach to the Treatment of Mammary Gland Hyperplasia by experiment on mammary gland hyperplasia.

目的：探讨中药经皮给药治疗乳腺增生病的动物实验研究思路。

The bPRL mammary gland expression vectors were transfected into COS-7 (African green monkey kidney cell) and HC-11 (epithelial cell of mouse mammary gland) cells mediated with lipofectin, respectively. The transfected cells were cultured and harvested. RT-PCR analysis showed that bPRL mRNA was present in both COS-7 and HC-11 cells.

将构建的乳腺特异的表达载体，分别转染COS-7细胞株和HC-11细胞株，收集转染培养后的COS-7和HC-11细胞，通过RT-PCR实验，证实转染的细胞中在mRNA水平均获得了表达。

About the problem of prostitution, the abolishment of prostitution becomes the cry of feminism in the movement.

对于贞操观念的极端化要求——节烈观，当时的女性主义者均一致予以彻底的批判。

In feminism he found an "amazonian" element.

在女权主义，他找到了一位"亚马孙"自供状。

This paper analyses the development of feminism in virginia woolf's a room of one's own.

本文从女性主义发展的角度对弗吉尼亚·伍尔夫的《1间自己的房间》的内容进行了分析。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"