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Third-rate make-up is the face make-up; second-rate make-up make-up is spiritual; class make-up make-up of life.

三流的化妆是脸上的化妆；二流的化妆是精神的化妆；一流的化妆是生命的化妆。

There we sat among the thousand fools just like us But not so in love like us There we spoke of all our feelings And dreams were born like that And we hope for love like that Now your dreams have changed, we're far apart I don't know when or where to start leading you from my mind I want to stand with you again I hope to find you here again in the eternal sunshine *I'll search for the orange moon that lit up our love on the ocean while i held your hand Now watch all the waves from the stones where we gazed to the golden sun If i see the horizon glow just the same as it did when we stood on the hill I'll make the arrangements, just wait on that corner for me If you drop me a line I'll be sure to make these arrangements* Lay your head upon my shoulder I'll sing a tune or three Sweet melodies for thee Softly speak to say what i meant Why do you leave My heart is at your sleeve Well I'd like to share three words again practise these words with you again if you think is possible and maybe i can pull you close whisper in your ear again that i want you in my life forever I'll search for the orange moon that lit up our love on the ocean while i held your hand Now watch all the waves from the stones where we gazed to the golden sun If i see the horizon glow just the same as it did when we stood on the hill I'll make the arrangements, just wait on that corner for me You're like summer rain that cools me, it sooths me You are the person that I pray for every day Maybe we'll sit on those stairs once again Maybe we won't turn down have to pretend You will be here in my imaginings, they'reimaginings I'll search for the orange moon that lit up our love on the ocean while i held your hand Now watch all the waves from the stones where we gazed to the golden sun If i see the horizon glow just the same as it did when we stood on the hill I'll make the arrangements, just wait on that corner for me If you drop me a line I'll be sure to make these arrangements* If you drop me a line I'll be sure to make these arrangements

我们坐在有1000间我们就像傻瓜但并非所有的爱都像我们一样在那里，我们谈的都是我们的感受与梦想这样诞生我们希望这样的爱现在你的梦想已经改变，我们很遥远我不知道何时何地开始你开始疏远我我想再次与你在一起我希望在这里再次找到你在永恒阳光*我要寻找的橙色月亮这点燃了在海洋我们的爱虽然我认为你的手从现在看所有的石头波我们凝视的金太阳如果我看到地平线辉光一样因为它没有当我们站在山上我会作出安排的，只是等待，为我的角落如果你删除我行我一定要作出这些安排*把你的头后，我的肩膀我会唱一调或3 为你甜蜜的旋律说话轻声地说，我的意思你为什么离开我的心在你的袖口那么我想再次分享三个词实行这些话你再次如果你认为是可能的或许我可以把你关闭在你的耳朵小声一次，我希望在我的生活中你永远我要寻找的橙色月亮这点燃了在海洋我们的爱虽然我认为你的手从现在看所有的石头波我们凝视的金太阳如果我看到地平线辉光一样因为它没有当我们站在山上我会作出安排的，只是等待，为我的角落你喜欢夏天雨水，冷却我一样，我也sooths 你是我的人，每天祈祷也许我们会坐在楼梯上的再次也许我们不会拒绝有假装在这里，您将在我的想像，they'reimaginings 我要寻找的橙色月亮这点燃了在海洋我们的爱虽然我认为你的手从现在看所有的石头波我们凝视的金太阳如果我看到地平线辉光一样因为它没有当我们站在山上我会作出安排的，只是等待，为我的角落如果你删除我行我一定要作出这些安排*如果你删除我行我一定要作出这些安排

Uh on me on me.. whatcha gonna do with all that junk all junk inside your trunk i'ma get get get get you drunk get you love drunk off my hump whatcha gonna do with all that ass all that ass inside your jeans i'ma make make make make you scream make you scream make you scream coz of my humps my hump my hump my hump my hump my hump my hump my lovely lady lumps check it out i met a girl down at the disco she said hey hey hey ya lets go i can be ya baby, you could be my honey lets spend time not money and mix your milk with my coco puff milky milky coco mix your milk with my coco puff milky milky riiiiight... they say i'm really sexy the boys they wanna sex me they always standin next to me always dancin next to me tryna feel my hump hump lookin at my lump lump you can look but you can't touch it if you touch it i'ma start some drama you don't want no drama no no drama no no no no drama so don't so don't you aint my man boy i'm just tryna dance boy and move my hump my hump my hump my hump my hump my hump my hump my hump my hump my hump my hump my lovely lady lumps my lovely lady lumps my lovely lady lumps in the back and in the front my loving got you..

你可以当我的甜心我们一起杀时间不是钱把你的牛奶跟我的可可混在一起奶味的可可你的牛奶跟我的可可混在一起牛奶牛奶是的他们说我很性感那些男孩麼都想干我他们永远都站在我身边永远都在我身边跳舞试著抚摸我的双峰双峰看著我的双峰双峰你可以看但你不能摸你如果摸了我就会开始生气你不想要我生气不不要我生气不不不不要我生气所以不要拉著我的手你不是我的男人我只是想要跳舞还有动我的双峰我的双峰我的双峰我的双峰我的双峰我的双峰我的双峰我的双峰我的双峰我的双峰我的双峰我可爱的女性双峰我可爱的女性双峰我可爱的女性双峰前面跟后面你被我的爱迷住它让我为他花钱喔~把你的钱都花在我身上。。。

One Wild Night It's a hot night, the natives are restless We're sweating by the light of the moon There's a voodoo mojo brewing at the go-go That could knock a witch off her broom We slither on in and shed our skin Make our way into the bump and the grind So I'm passing by, she gives me the eye So I stop to give her a light Chorus: One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone And she left my heart with vertigo) One wild, one wild, one wild, one wild night Havin' as much fun as you can in your clothes Margarita had me feelin' alright It just might be that I found religion I've been on my knees for half of the night Then I'm rolling the bones with Jimmy 'no dice' Gonna take him for a couple weeks' pay Man, if you lose this roll I take your girlfriend home So I stopped But you're not gonna believe who comes walking out Chorus: One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone And she left my heart with vertigo) One wild night (hey, c'est la vie) One wild night One wild night (life is for the living so You gotta live it up, come on let's go) One wild, one wild, one wild, one wild night Guitar solo Chorus: One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone And she left my heart with vertigo) One wild night (hey, c'est la vie) One wild night One wild night (life if for the living so You gotta live it up, come on let's go) One wild, one wild, one wild, one.. One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone) For one wild night One wild night (hey,c'est la vie) One wild night One wild night All we've got is one wild night One wild night (4x) One wild night blinded by the moonlight One wild night 24 hours of midnight One wild night stepped into the twilight zone for One wild, one wild, one wild, one wild night.

一狂放的夜它是热的夜，当地人是不安定的由那里月亮的光冒汗是巫毒教mojo酿造在可能敲巫婆她的笤帚的去去的我们我们滑行并且流洒我们的皮肤做我们的方式成爆沸和研磨，因此我通过，她给我眼睛，因此我停下来给她轻的合唱：一狂放的夜一狂放的夜(24个小时午夜)一狂放的夜(我跨步入暮色区域和她左我的心脏以眩晕)一狂放，一狂放，一狂放，一狂放的夜 Havin同样多乐趣，象您在您的衣裳玛格丽塔酒能有我feelin它也许行是我发现了我是在我的膝盖为夜的一半的宗教我然后滚动骨头与吉米'没有模子' 去采取他为几星期的薪水人，如果您丢失我采取您的女朋友家的这卷，因此我停止了，但您不相信谁来走出去 Chorus： One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone And she left my heart with vertigo) One wild night (hey， c'est la vie) One wild night One wild night (life is for the living so You gotta live it up， come on let's go) One wild， one wild， one wild， one wild night Guitar solo Chorus：一狂放的夜一狂放的夜(24个小时午夜)一狂放的夜(我跨步入暮色区域和她左我的心脏以眩晕)一狂放的夜(嘿， c'est la竞争)一狂放的夜一狂放的夜(生活，如果为生活，因此您得到居住它，进展我们是)一狂放，一个狂放，一个狂放，一个一狂放的夜一狂放的夜(24个小时午夜)一狂放的夜为一狂放夜一个狂放的夜(嘿， c'est la竞争)一狂放的夜一狂放的夜我们有的全部是一狂放的夜一狂放的夜(4x)一狂放的夜由月光蒙蔽了一狂放的夜24个小时午夜狂放的夜跨步入暮色区域为一狂放的一，一狂放，一狂放，一狂放的夜。

Make up 40% sugar juice now, using sugar volume and raw material to compare is 1: 0.7, 0.2% citric acid are added in sugar juice, because watermelon skin organization is harder, can appear candy law to heat with boil make, cast the watermelon skin that cent has cut into to boil make, it is OK to begin big a crucial moment heats, make its fast evaporate moisture, boil raw material to become transparent all the time, when sugar juice concentrates ceaselessly and showing syrup, should be to use small fire to undertake heating, lest produce anxious saccharify to make product darkly Brown, determine sugar juice chroma is achieved 70% when should stop to heat.

现配制40%糖液，用糖量与原料比是1：0.7，在糖液中加入0.2%柠檬酸，因西瓜皮组织比较坚硬，可用一次透糖法加热煮制，把分切好的西瓜皮投进一起煮制，开始可以大火候加热，让其快速蒸发水分，一直煮到原料变得透明，糖液不断浓缩而呈糖浆时，应是用小火进行加热，以免产生焦糖化使产品呈黑褐色，测定糖液浓度达到70%时就应停止加热。

Make signal be called binary system numeral to make for the make of binary system signal, the binary system make and is divided into a binary system to move key to control(2 ASK), the binary system Pin move key to control(2 FSK), the binary system mutually move key to control(2 PSK) with bad cent the binary system mutually move key to control(2 DPSK) etc. variety basic type, this topic main is numeral the frequency make and call Pin to move key to control.

调制信号为二进制信号的调制称为二进制数字调制，二进制调制又分为二进制幅移键控（2ASK）、二进制频移键控（2FSK）、二进制相移键控（2PSK）和差分二进制相移键控（2DPSK）等多种基本的类型，本课题主要是数字频率调制又称频移键控。

A steel bar is washed and dried, and then put into a glass pipe which is sealed to obtain stirring magneton; a mixed solvent of Gamma-methacrylate oxide propyl trimethoxy silane and methanol is prepared and added with an organic base to obtain a pre-bonding solvent; the stirring magneton is immerged into the pre-bounding solvent, and the outer surface of the glass pipe of the stirring magneton is bonded with a layer of silane substance with alkenyl; a reactive monomer mixture and a pore-forming agent mixed solvent are evenly mixed and then added into a container, the stirring magneton after the pre-bounding is put into the container which is sealed and then put into a water bath; after reaction, the container is removed, the stirring magneton coated with a polymer is taken out and put into a extractor and extracted by using a solvent; or the stir bar is immersed into the solvent till no impurity can be checked in the solvent, thereby obtaining the product.

将铁条清洗干燥，放入玻璃管内，密封得搅拌磁子；配制γ-甲基丙烯酸氧丙基三甲氧基硅烷与甲醇的混合溶液，加入有机碱得预键合溶液，将搅拌磁子浸入预键合溶液，在搅拌磁子的玻璃管外壁键合一层带烯基的硅烷化物质；将反应单体混合剂和致孔剂混合溶液混匀后倒入容器中，将预键合后的搅拌磁子放入容器，密封后置于水浴中，反应后除去容器，取出涂有聚合物的搅拌磁子，置于提取器中，以溶剂提取，或将搅拌棒置于溶剂中浸泡至溶剂中无杂质检出，得产物。

The carbons in explosives are decomposed into gas-like carbon atoms in the detonation reaction zone, then unoxidized carbon atoms coagulate into carbon liquid droplets and finally consolidate into solid diamonds The new mechanism differs from one conclusion that carbons of explosives are at first transformed into solid graphites, then into diamonds, and also differs from the conclusion that gaseous carbons coagulate into liquid droplets in the reaction zone, then crystalize into diamonds in Taylor rarefaction wave zone.

修正了有关炸药中的碳在反应区内先转变为石墨，然后石墨再发生相变生成金刚石的论点以及认为在反应区内气态碳凝聚为碳液滴，液滴在Taylor稀疏波区凝固成金刚石的论点。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Bounce [verse 1] I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Said you're going with your boys today and "Don't worry cause I'll be home late" But I know you're creepin' out there, cheatin', You've been freakin' babe So let me tell you how it's going to be And you can run game but not on me boy So who the hell are you trying to kid You know this is what I did I called your pager and your two-way boy but no response Start looking and I found you in some other women's arms Don't try to front boy cause I saw you there with my own eyes Just get your stuff boy cause I'm tired of hearing all your lies I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Baby boy I know you'll never find No stuff as good as mine She can't work you like I did You can look but you know I got that good shit I know that you're hating it But you better stay with the one you're with You made your bed and now you have to sleep in it baby I called your pager and your two-way boy but no response Start looking and found you in some other women's arms Don't try to front boy cause I saw you there with my own eyes Just get your stuff boy cause I'm tired of hearing all your lies Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door I ain't gonna take this no more I ain't gonna take this no more Bounce baby out the door Bounce baby out the door Get up and move Get up

弹跳 [韵文1 ] I是在哪里也不会采取这种不弹跳宝宝出了门起床及动议不要让我的行为傻瓜刚刚反弹的婴儿出了门弹跳宝宝出了门 I是在哪里也不会采取这种不弹跳宝宝出了门起床及动议不要让我的行为傻瓜说你去你的男孩今天"不要担心，造成我会回家太晚"不过，我知道你在外面creepin '在那里， cheatin '，你已经freakin '贝比因此，让我告诉你如何这将是你可以运行游戏，而不是对我的男孩那么，谁该下地狱，你要多Kid 你知道这是什么，我我呼吁你的传呼机和你的双程男孩，但不作回应开始寻找，我发现你在其他一些妇女的怀里不要试图到前面的男孩引起我看到你，有我自己的眼睛刚刚得到你的东西男孩引起我已经厌倦了听你的谎言 I是在哪里也不会采取这种不弹跳宝宝出了门起床及动议不要让我的行为傻瓜刚刚反弹的婴儿出了门弹跳宝宝出了门 I是在哪里也不会采取这种不弹跳宝宝出了门起床及动议不要让我的行为傻瓜男婴我知道你永远也找不到没有任何东西一样好矿山她不能工作，你喜欢我你可以看看，但你知道，我还没好为Shit 我知道你恨它不过，你最好留一个您与你了你的床，现在你必须睡在它婴儿我呼吁你的传呼机和你的双程男孩，但不作回应开始寻找和发现，你在其他一些妇女的怀里不要试图到前面的男孩引起我看到你，有我自己的眼睛刚刚得到你的东西男孩引起我已经厌倦了听你的谎言弹跳宝宝出了门 I是在哪里也不会采取这种不弹跳宝宝出了门起床及动议不要让我的行为傻瓜刚刚反弹的婴儿出了门 I是在哪里也不会采取这种不 I是在哪里也不会采取这种不弹跳宝宝出了门弹跳宝宝出了门起床及动议起床

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48