查询词典 macrophages

Was presented in 12 cases (24%) by bacterial isolation. By in-situ hybridization, PCV2 signals were chiefly distributed in interstitial and necrotic lesions, and the positive signals could be found in macrophages-like cells and necrotic debris. PRRSV signals were mainly located at interstitial lesions and alveolar wall, and the positive signals could be found in macrophages-like cells and epithelial cells of alveoli. Swine influenza virus could be found in interstitial and necrotizing lesions, and the signals could be found in macrophages-like cells, and epithelial cells of terminal bronchiole and alveoli.

另运用原位杂交法对PCV2、PRRSV及猪流行性感冒病毒进行检测，结果发现PCV2主要分布於PNP的间质及坏死病灶区，并能於肺泡及终末细支气管上皮细胞、巨噬细胞与坏死细胞碎片皆可见有病毒核酸；PRRSV则多分布於间质病灶区及肺泡壁处，能於巨噬细胞与肺泡上皮细胞见到病毒核酸；SIV则存在於间质及坏死病灶区，且能於肺泡及终末细支气管上皮细胞与巨噬细胞见有病毒核酸。

The number of lymphocytes in lymph node,lymphatic cord and lymphatic sinusoid decreased remarkably,whereas the number of macrophages increased significantly. Plenty of macrophages were gathered together, resulting in cellular outline to be ambiguous, which phagocytosised amounts of hemosiderin granules.Their movement tendency was disappeared and antigen procession function was lost.

结果显示，一些病猪的淋巴结、淋巴索和淋巴窦内的淋巴细胞数量显著减少，巨噬细胞的数量大幅度增加；大量吞噬有含铁血黄素颗粒的巨噬细胞聚集在一起，连成一片，致使细胞轮廓不清；它们的趋化运动能力和吞噬处理抗原的机能丧失。

Mast cells and macrophages may be involved in the development of PH and/or the lesion caused by PH accelerate the accumulation and activation of mast cell and macrophages.

结论肥大细胞及巨噬细胞参与了肺动脉高压的形成或/和肺动脉高压造成的损伤促进了肥大细胞及巨噬细胞的聚集与活化。

RNAs were obtained from noninfected and Pr4- and Pr4△35-infected macrophages at 3, 8, and 24 hpi and hybridized to a radiolabeled swine IFN-α cDNA probe.

RNAs 是获得从非受传染的和 Pr4-而且 Pr4 △ 35-受传染的 macrophages 在 3 点， 8，和 24 hpi 而且对 radiolabeled 猪 IFN 使杂交-α cDNA 探针。

Methods Peritoneal macrophages of guinea pigs were infected in vitro by three different Leptospira strains, the virulent Leptospira interrogans serovar Lai type strain Lai, the avirulent L. interrogans serovar Lai type strain IPAV, and the nonpathogenic L. biflexa serovar Patoc type strain PatocⅠ, respectively, and heat inactivated Staphylococcus epidermidis was added 0.5, 1.5, 3 and 6 h after infection and incubated for 30 min. The effect of Leptospira on the phagocytosis of macrophage was evaluated by the inactivated Staphylococcus epidermidis phagocytosis rate and phagocytosis index. Phagocytosis and ultrastructure of peritoneal macrophages were observed by transmission electron microscopy 3 h after infection, and changes of cytoskeleton of the macrophages were observed by laser scanning confocal microscopy.

用三种不同毒力的钩体（致病性问号钩端螺旋体赖型有毒株Lai株、赖型无毒株IPAV株以及非致病性双曲钩端螺旋体Patoc型PatocⅠ株）分别感染体外培养的豚鼠腹腔巨噬细胞，并分别于感染后0.5、1.5、3和6 h加入热灭活表皮葡萄球菌孵育30 min，通过计算巨噬细胞对灭活表皮葡萄球菌的吞噬率和吞噬指数，检测钩体对巨噬细胞吞噬功能的影响；感染后3 h透射电镜观察巨噬细胞对钩体的吞噬、降解和细胞超微结构的变化；用激光共聚焦显微镜观察细胞对钩体的吞噬和巨噬细胞细胞骨架的变化。

Objective:To study the effect of peritoneal macrophages on peritonitis of rats with experimental pancreatitis.

目的： 研究腹腔巨噬细胞(peritoneal macrophages， PMs)对实验性胰腺炎大鼠腹腔炎症反应的影响。

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h，MTT法检测ox-LDL对RAW264.7细胞生长的影响；流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡；用特异性siRNA沉默Daxx在RAW264.7细胞中的表达，通过AO／EB染色观察基因沉默后的细胞凋亡形态学改变；高效液相色谱检测细胞内胆固醇含量；油红O染色观察细胞内脂滴的形成情况；Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况；用Western Blot印迹法检测caveolin-1蛋白的表达。

In the first section, the effect of sodium houttuyfonate on the level of lysozyme secreted by macrophages was first tested by bacteriolytic method; then the effect of sodium houttuyfonate on the level of tartaric acid resistant acid phosphatase produced in macrophages was investigated by analyzing the phenol generated in the reaction that was catalyzed by the enzyme; the third, the effect of sodium houttuyfonate on the phagocytic activity of macrophages was observed by microscopy; the forth, the effect of sodium houttuyfonate on the production of complement C4 from macrophages was tested by hemolytic method; the fifth, the effect of sodium houttuyfonate on the expression of C3b receptors on the surface of macrophages was analyzed by observing the formation percentage of zymosan wreath; the sixth, the effect of sodium houttuyfonate on the respiratory burst in macrophages was tested by flow cytometry; the seventh, the effect of sodium houttuyfonate on the production of IL-1α and IL-1β from macrophages was tested by ELISA method.

在这一章中，首先，采用溶菌法考察合成鱼腥草素对巨噬细胞分泌溶菌酶水平的影响；其次，采用游离酚法研究合成鱼腥草素对巨噬细胞产生的耐酒石酸酸性磷酸酶水平的影响；第三，采用显微镜观察法考察合成鱼腥草素对巨噬细胞吞噬功能的影响；第四，采用溶血法测定合成鱼腥草素对巨噬细胞产生的补体C4水平的影响；第五，采用酵母花环法检测合成鱼腥草素对巨噬细胞表面C3b受体水平的影响；第六，采用流式细胞术法观察合成鱼腥草素对巨噬细胞呼吸爆发的影响；第七，采用ELISA法探讨合成鱼腥草素对巨噬细胞产生IL-1α及IL-1β水平的影响。

Morphologically, the macrophages treated with NO and O-LDL presented shrinkage, nucleus fragmentation, and cytoplasm condensation; DNA ladder was visualized by agarose gel electrophoresis; Characteristic apoptosis peak of macrophages was showed by flow cytometry; Ultrastructure, The chromatin condensed and marginated to form dense chromatin fragmentation; The cytoplasm concentrated and vacuolized; The lysosomes and mitochondria of apoptosis macrophages increased clearly.

电镜观察超微结构变化：染色质固缩边聚，形成致密、均质的染色质块，胞质浓缩、电子密度升高并空泡化；凋亡巨噬细胞溶酶体、线粒体增多；细胞突起缩短减少，但巨噬细胞特有的板状伪足不消失。

In mouse peritoneal macrophages. Methods Mouse peritoneal macrophages were cultured in the presence of heat-killed yeast-phase PM for 24 h, and the average fluorescence intensity of TLR-2, TLR-4, and Dectin-1 in the macrophages was detected using flow cytometry. Fluorescent staining of the macrophages was performed to observe the fluorescence of TLR-2, TLR-4, and Dectin-1 with confocal microscopy. TNF-α mRNA in the cell culture supernatant was measured with real-time PCR, and TNF-α protein detected using enzyme-linked immunosorbent assay.

马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h，采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度；共聚焦显微镜观察荧光染色的受体；ELISA法测定培养液上清中TNF-α的浓度；Real time PCR检测不同时间段TNF-α的mRNA表达。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。