查询词典 lysing

The self-made lysing solution can lyse red blood cells completely, but not destroy leukocytes and influence the expression of surface antigens on lymphocytes, monocytes and granulocytes significantly.

自制流式细胞仪专用溶血素具有很好的红细胞溶解效果，但不破坏白细胞，且对淋巴细胞、单核细胞和粒细胞群表面抗原的表达无明显影响作用。

Experimentally, multi-pores are observed on leukocyte lysed with 3D electrodes and just single pore with planar electrodes. This is believed to be due to more uniform electric field of 3D electrodes than that of planar electrodes. Intracellular elements are extracted through the membrane as observed by increased intensity of Trypan blue when diluted by saline solution. We also demonstrate the lysing percentages at same flow velocity to explain the performance of 3D electrode is superior to that of planar electrode.

在实验方面，可发现细胞在3D 电极的形况下，是有多个破孔出现，有别於在平面电极的单个破洞，也发现当细胞出现破孔时，经由Trypan blue的辅助观察以及在食盐水稀释情况下，细胞内液是往外流出的，另外由裂解比率的结果，3D电极也是优於平面电极。

A method of extracting nucleic acids from blood comprises contacting blood cells, preferably after lysing with an activated solid phase at one pH to immobilise the nucleic acids and then removing the nucleic acids at a higher pH when the charge has been reversed or neutralised.

一种从血液中提取核酸的方法，该方法包括：将血细胞，最好于细胞裂解之后，在一个pH值时与活化固相接触以固定核酸，然后在电荷逆转或变为电中性时在较高pH值下取出核酸。

In conclusion, all lysing procedures that we used induced substantial loss of leukocytes and CD34+ cells.

我们用的所有裂解方法均导致白细胞和CD34+细胞的大量丢失。

Several methods for measuring the lysing cycle and burst size of the cyanophage were tested. It was established that cell counting method was more convenient and exact for confirming the lysing cycle.

我们研究了确定该噬藻体裂解周期和释放量的多种方法，认为细胞计数法是测量裂解周期既方便又准确的方法，而采用1PFU的感染可获得噬藻体的准确释放量。

The results indicated that the lysing curve of host cells was consistent with the one step growth curve of cyanophage, thus it was more convenient to substitute measuring the lysing curve of host cells for the one step growth curve measurement of cyanophage.

近年来，随着浮游病毒的认识的深入，人们认识到浮游病毒对水体中初级生产力的影响是巨大的[1] ，其主要证据就是发现噬藻体在海洋蓝藻的种群控制上发挥着重要作用[2 ] 。

Since preanalytic lysing of erythrocytes remains critical in flow cytometry, we inestigated the influence of four lysing procedures on the quantification of leukocyte and CD34+ cells in hematopoietic cell transplants.

文献摘要中文：因为预先裂解残余的红细胞在流式细胞分析时是关键的，我们调查了四种裂解方法对用于造血细胞移植的白细胞和CD34+细胞量的影响。

Methods: K3 EDTA peripheral blood of 40 healthy volunteers was dealt with a self-made lysing solution and a FACS Lysing solution, and then the lysing effect on red blood cells and the influence on CD14(superscript +), CD45(superscript +) on lymphocytes, monocytes and granulocytes were detected.

应用流式细胞术对40份K3 EDTA抗凝外周血分别用自制流式细胞仪专用溶血素与FACS溶血素处理后，检测其对血液红细胞的溶解效果及对淋巴细胞、单核细胞和粒细胞表面特异性抗原CD14、CD45表达率的影响。

Methods: K3 EDTA peripheral blood of 40 healthy volunteers was dealt with a selfmade lysing solution and a FACS Lysing solution, and then the lysing effect on red blood cells and the influence on CD14+, CD45+ on lymphocytes, monocytes and granulocytes were detected.

应用流式细胞术对40份K3 EDTA抗凝外周血分别用自制流式细胞仪专用溶血素与FACS溶血素处理后，检测其对血液红细胞的溶解效果及对淋巴细胞、单核细胞和粒细胞表面特异性抗原CD14+、CD45+表达率的影响。

The high rate of protoplasts formation and fusants regeneration could been obtained for strain F46 lysing 75min with 10 mg/mL snailase and 10 mg/mL lysozyme (1:1) in 35℃water bath, and for strains SC1 and SC11 lysing 60 min and 100min with 10 mg/mL lysozyme and 15 mg/mL lysozyme respectively in 35℃water bath.

对菌株SC1和SC11，分别用10mg/mL和15mg/mL溶菌酶酶解60min和100min较合适；对菌株F46，以蜗牛酶(10mg/mL)和溶菌酶(10mg/mL)体积比为1：1的混合酶液酶解75min为宜。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。