查询词典 lymphocyte
- 与 lymphocyte 相关的网络例句 [注:此内容来源于网络,仅供参考]
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These results suggested that some cytokines alone or in combination might be the activators of lymphocyte adhesion in inflammation.
综合以上结果,我们推测:炎症发生后,淋巴细胞首先具备了与ECM粘附的潜能,这种潜能可能是细胞粘附分子的表达发生了变化。
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Lymphocyte trapping is another mechanism of action of oil adjuvants.
淋巴细胞的俘获是另一个油佐剂的机理作用。
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Blood samples were drawn from every patient at admission to the detect the leukocyte counts in peripheral blood, including total leukocyte count, neutrophil count, eosinophil count, lymphocyte count, monocyte count and basophil count.
对所有患者进行人体测量(体质量指数与收缩压、舒张压的测量),检测患者的外周血中白细胞计数(包括白细胞总数、中性粒细胞计数、嗜酸性粒细胞计数、淋巴细胞计数、单核细胞计数和嗜碱性粒细胞计数)。
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Methods After Candida albicans were inoculated into the specific pathogen free mice, the total amount of Candidas in cecum and the amount of which attached to the mucosal membrane were counted at different interent intervals. The lymphocyte proliferation in Peyer's patch and in lamina proper was shown by BrdU incorporation, meanwhile B cells isotype switch in PP was investigated. IgA plasma cells were shown by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.
采用无特殊病原菌动物经口喂入白念菌,在不同时相处死后,观察肠内白念菌总数及肠粘膜表面白念菌粘附数量;取肠系膜淋巴结做白念菌选择培养,观察移位体内发生率;采用Brdu体内掺入显示局部粘膜淋巴细胞的增殖情况;流式细胞计数潘伊尔氏结细胞表面IgA阳性率;免疫组化染色后计数固有层中IgA浆细胞数量变化;ELISA法测定肠粘液中特异抗白念菌IgA含量。
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By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.
为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus,FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗,本研究通过定点突变方法和PCR扩增方法,获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D,将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接,分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D;对重组质粒进行序列测定、分析,并将重组质粒分别转染BHK-21细胞,通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达,用电子显微镜观察病毒空衣壳的组装;为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果,将重组质粒经肌肉注射方法接种豚鼠,并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫,第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株;随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛,三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。
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By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.
为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus,FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲,本研究通过定点突变方法和PCR扩增方法,获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D,将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接,分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D;对重组质粒进行序列测定、分析,并将重组质粒分别转染BHK-21细胞,通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达,用电子显微镜观察病毒空衣壳的组装;为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果,将重组质粒经肌肉注射方法接种豚鼠,并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫,第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株:随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛,三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。
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Convincing evidence indicates that matching for HLA class I cross-reactive groups rather than conventional HLA antigen matching may result in improved renal graft survival. T and B lymphocyte crossmatch before transplantation was done to determine whether there is any human leukocyte antigen antibodies to the graft.
等待肾脏移植的病人数量有限,然而要找到HLA配对很好的机率真的很低,后来有新的策略,将大量的HLA等位基因缩减为一些决定常见的HLA抗原的少量联系紧密的HLA等位基因组,称为交叉反应组。
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We retrospectively determined the correlation of results of lymphocyte crossmatch tests by direct complement-dependent cytotoxicity, to the outcomes of 585 consecutive ABO-identical and human leukocyte antigen-mismatched living donor liver transplants (male:female=276:309; median age, 18 years).
我们回顾性研究了连续585名ABO血型相容但HLA错配的活体肝移植的结果(男性:女性=276:309;中位年龄18岁),并研究采用补体依赖细胞毒性试验来检测淋巴细胞交叉配型结果与其的关系。
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ObjectiveTo probe into the hyperplasy effect of different curcumine administered approaches for the splenic lymphocyte of mice.
目的探讨姜黄素3种不同给药途径对小鼠脾淋巴细胞增殖的影响。
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ObjectiveTo probe into the hyperplasy effect of different curcumine administered approaches for the splenic lymphocyte of mice.
目的探讨姜黄素3种不同给药途径对小鼠脾淋巴细胞增殖的影响。方法通过噻唑蓝比色法比较姜黄素3种给药途径对小鼠脾淋巴细胞增殖的影响。
- 推荐网络例句
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I will endeavour to find you some assistance.
我尽力帮你找人帮忙。
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At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.
起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像,后来回台湾,甚至去年在北京,居然也四处看见她的海报,才惊讶她的魅力之大。
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Ah may dee:You are chinese living in a democratic country.
你是居住在民主国家的中国人吧。