查询词典 lymphocyte

Results PE was found to be able to stimulate the proliferation of murine splenocyte in the absence of accessory cells. Accessory cell and its supernatant from AC stimulated with PE was able to enhance the proliferation of lymphocyte induced by PE.

结果 PE在0.01～100ng/ml浓度范围能刺激鼠脾淋巴细胞增殖，PE的丝裂原作用无需粘附细胞的辅助，但粘附细胞及其粘附细胞的PE刺激上清能协同PE对鼠脾淋巴细胞的刺激作用。

T cell subsets of peripheral blood lymphocyte were studied in 21 splenomegalia patientswith advanced schistosomiasis japonica,24 splenectiomized patients with AS,and 20 normal persons.The results showed that the rates of CD_4￣+,CD_3￣+ and ratio of CD_4￣+/CD_8￣+were significantly decreased in both groups of patients,as compared with thecontrols.

21例晚期血吸虫病巨脾型患者和24例切脾患者、20例正常人外周血T细胞亚群检测结果表明：晚血患者两组CD_3~＋、CD_4~＋细胞百分率及CD_4~＋／CD_8~＋比值显著低于正常人（P＜0.01），巨脾组与切脾组CD_3~＋、CD_4~＋、CD_8~＋细胞百分率和CD_4~＋／CD_8~＋比值差异无显著性（P＞0.05）。

Methods At first, retrovirus vectors encoding IFN-γ or IL-4 gene were constructed. They were transfected into PA317 packaging cells by lipofectamine, PA317IFN-γ and PA317IL-4 cells were obtained. C6 glioma cells were infected with replication deficiency retrovius containing IFN-γ or IL-4 gene, cell morphology、cell growth curve and cloning efficiency assay were examined. The intracranial C6 glioma animal model was established in immunocompetent Wistar rats. PA317IFN-γ and PA317IL-4 cells were stereotactically implanted into the tumor areas alone or together. The survival of tumor bearing rats were examined, tumor volumes were measured and immunohistochemical analyses of CD4〓 and CD8〓 T lymphocyte infiltration were performed.

构建和鉴定携带IL-4或IFN-γ基因的逆转录病毒载体，将其导入逆转录病毒包装细胞PA317，通过G418抗性筛选，得到携带目的基因的包装细胞并鉴定；应用携带IL-4或IFN-γ基因的复制缺陷型逆转录病毒感染C6胶质瘤细胞，观察对C6胶质瘤细胞形态、细胞生长曲线和克隆形成率的影响；建立C6胶质瘤大鼠脑内移植动物模型，将携带IL-4和IFN-γ基因的逆转录病毒包装细胞分别或联合注射到脑内荷瘤大鼠的肿瘤组织中，观察其治疗作用，并初步探讨其机制。

Methods Human umbilical cord blood samples were obtained sterilely from healthy parturients. MSCs were isolated by lymphocyte separation medium (density 1.077g/ml), MSCs were obtained by adhere culture. The surface antigen expression of MSCs was detected by flow cytometry.

将无菌条件下采集健康产妇脐带血，用相对密度为1.077g/ml的人淋巴细胞分离液分离脐血单个核细胞，利用间充质干细胞贴壁生长的特性获得MSCs，流式细胞仪检测其表面抗原标志。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后，利用SABC法检测CD80的表达；MTT法测定肿瘤细胞体外增殖能力；将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度；在同源淋巴细胞肿瘤细胞混合培养后，通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

Seven inactivated tetravalent vaccines of Riemerella anatipestifer,of which three (1#,3#,7#) employed oil-emulsion,two(4#,6#) employed propolis,two(8#,10#) employed aluminum hydroxide as their basic adjuvants,were developed and compared by the methods as follows:challenges were performed by homologous virulent RA strains to measure their protective abilities;indirect enzyme-linked immunosorbent assay was used to monitor antibody production;lymphocyte transformation test was performed to study the expression of cell-mediated immunity.

本实验通过对供试鸭的攻毒保护试验、通过用ELISA方法对供试鸭特异性抗体消长规律的研究以及通过用~3H-TdR掺入法对供试鸭T细胞免疫水平的检测三个方面对所研制的七种不同佐剂类型的鸭疫里氏杆菌四价灭活苗的免疫原性进行了比较研究。

To observe the change of behavior index and mensurate the contents of plasma CORT, CRH and ACTH by using radiation immunity methods, and observed the change of immunity function by testing IL-2 and lymphocyte multiplication produced by spleen cell. At the same time, the author examined the change of thalamencephalon CRH mRNA and pituitary ACTH mRNA.

观察行为学指标的变化，利用放免法测定血浆皮质酮、下丘脑促肾上腺皮质激素释放激素和血浆促肾上腺皮质激素的含量，并通过脾细胞产生白细胞介素2（IL-2）水平和淋巴细胞增殖试验观察大鼠免疫功能的变化，同时检测大鼠下丘脑CRH mRNA和垂体ACTHmRNA含量变化。

Neural stem cells are inherent in immunogenicity. When transplanted into traumatically injured brain, they can induce lymphocyte infiltration.

神经干细胞具有一定免疫原性，当移植到外伤性脑损伤病灶中时，可以诱发局部淋巴细胞浸润。

Objective The process of immune response to neural stem cell allograft has not been fully understood. This study was designed to investigate the lymphocyte infiltration induced by transplantation of neural stem cells into traumatically injured brain.

目的 探讨在外伤性脑损伤的条件下，移植同种异体神经干细胞所诱发的淋巴细胞浸润现象。

Further,with indirect immunohistochemistry technique,CD25(IL-2 receptor or IL-2R) cross-reaction has been identified on lymphocyte membrane of Ctenophaxyngodon idellus and Trionyx sinensis using mouse monoclonal antibody directed against Human Leucocyte Differentiation Antigen CD25(IL-2R).

进一步用抗人白细胞分化抗原CD25(IL-2受体，简称IL-2R)单克隆抗体进行免疫组化交叉反应提示草鱼、中华鳖淋巴细胞膜上含有与人类IL-2R类似功能和结构的物质。关键词：白细胞介素-2；白细胞介素-2受体；发育和比较免疫学；草鱼；中华鳖

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。