查询词典 lymphocyte

Routine detecting techniquefor cell immunity such as white blood count and differential, lymphocyte blastogenesis test has beenused, and combine with flow cell technology to detect the level of cell immunization after advancedimmunization at several important aspect, and tracking experiments to observe changes in the numberof the WBC and lymphocytes, lymphocyte proliferation, the dynamic changes of CD4~+ and CD8~+lymphocyte subsets andγ-IFN; and detected dynamic changes rules of IgG, IgM in humor andmucosa with indirect ELISA, then compare with ordinary immunization methods.

主要采用白细胞计数和分类计数、淋巴细胞转化试验等常规细胞免疫检测技术结合先进的流式细胞技术从细胞免疫的几个重要方面入手检测猪瘟超前免疫后细胞免疫的水平，并进行跟踪试验，观察其白细胞、淋巴细胞数目变化情况，淋巴细胞增殖情况，CD~(4+)和CD~(8+)淋巴细胞亚群及γ-IFN的动态变化规律；同时通过间接ELISA方法检测体液和黏膜中各IgG、IgM的动态变化规律，同时与普通免疫方法进行比较。

A specific antigen from group A streptococcal cell membrane and 1% PHA used as a stimulant to test the peripheral blood lymphocyte adherence inhibition percent and procoagulant activity in 30 cases of active rheumatic carditis, 24 cases of inactive rheumatic carditis and 38 cases of normal controls.

用A组乙型溶血链球菌特异性菌膜抗原和1%植物血凝素作为刺激物，分别测定38例健康对照组，30例风湿性心脏病风湿活动组，24例风湿性心脏病静止组的外周血淋巴细胞贴壁抑制百分率（Lymphocyte adherence inhibition percent， LAIP）和促凝血活性（Procoagulant activity， PCA）。

Results: As compared with those of the irradiation control groups, the rhCuZnSOD showed significant beneficial effects on immunocytes, these included enhancing the proliferative response of T lymphocyte to concanavalin A and the activity of natural killer cells in spleen, increasing the number of whole spleen lymphocyte, leucocyte and lymphocyte counts in peripheral blood.

与照射对照组比较，rhCuZnSOD可减轻电离辐射对小鼠免疫细胞的影响，其作用包括增强脾T淋巴细胞对刀豆蛋白A诱导的增殖反应，提高脾自然杀伤细胞的活性，升高全脾淋巴细胞及外周血白细胞和淋巴细胞的数量。

The studies on the capacities of immune responses of lymphocytes in peripheral blood and immune organs of Grass Carp (Ctenpharyngoden idellus Cuvier et Valencinnes) to PHA and SPA with incorporation of 〓H-TdR and active reaction of Acid α-Naphthyl Acetate Esterase indicated that there were T and B lymphocytes in peripheral blood of grass carp, but only T lymphocyte and no B lymphocyte in the thymus of grass carp. It was justified that the T lymphocytes in peripheral blood was originated from the thymus, and B lymphocyte could be from the spleen and anterior kidney of grass carp.

运用〓H-TdR掺入法和酸性醋酸萘酯酶活性检测法分别研究了草鱼外周血以及免疫器官中的淋巴细胞对PHA和SPA的免疫应答以及化学标志特征，发现草鱼外周血中存在着类似于哺乳动物的T、B两类不同的淋巴细胞亚群；同时也发现胸腺中只有T淋巴细胞，而无B淋巴细胞，提示了胸腺中淋巴细胞可能是外周血中T细胞的来源，而B淋巴细胞可能来自脾脏或前肾。

Methods The donor rat spleen was continuously perfused with WZD to select the best protocol. Then the lymphopoiesis was examined with mixed lymphocyte culture and the splenic cells were observed microscopically and immunohistochemically.

以WZD溶液持续灌注移植脾，筛选最佳方案，并观察灌注后混合淋巴细胞培养（mixed lymphocyte culture，MLC）淋巴细胞增殖情况及脾细胞的超微结构，以及灌注后移植脾细胞的免疫组化情况。

30U、P30W、P30S1 and P30S2 were isolated from PIP30, and P60U、P60W、P60S1 and P60S2 were isolated from PIP60, and P1W、P1S1 and P1S2 were isolated from PIWE1-10, and P10W、P10S1 and P10S2 were isolated from PIWE10-100, and P100U、P100W、P100S1 and P100S2 were isolated from PIWE100 using DEAE Sepharose F. F. The proliferation rates of lymphocyte in vitro of each polysaccharide fractions obtained during the isolation and purification were detected in order to observe their immune activities. The results showed that crude polysaccharides both obtained from ethanol precipitation and ultrafiltrate had the promoting effects on lymphocyte proliferation in various degrees. P30U、P30W、P30S1、P30S2、P60U、P60W、P60S2、P1S1、P1S2、P10S1、P10S2、P100U、P100W、P100S1 and P100S2 had the promoting effects on lymphocyte proliferation.

IP30经阴离子交换层析(DEAE-Sepharose Fast Flow)得到分级多糖P30U、P30W、P30S1和P30S2，PIP60经阴离子交换层析得到分级多糖P60U、P60W、P60S1和P60S2，PIWE1-10经阴离子交换层析得到分级多糖P1W、P1S1和P1S2，PIWE10-100经阴离子交换层析得到分级多糖P10W、P10S1和P10S2，PIWE100经阴离子交换层析得到分级多糖P100U、P100W、P100S1和P100S2；采用体外刺激淋巴细胞增殖实验对离子柱分级分离的多糖进行活性实验表明P30U、P30W、P30S1、P30S2、P60U、P60W、P60S2、P1S1、P1S2、P10S1、P10S2、P100U、P100W、P100S1和P100S2是有活性的。P60W、P60S1、P1S1、P10S1和P100S1经凝胶柱(Sephacryl S系列材料)层析获得五个纯多糖组分P60w1、P60s1、P1SP1、P10SP1和P100SP1。

CD8~+ cell is the main T lymphocyte subset in spleen, and B lymphocyte mainly is IgG~+ cell, moreover the amount of these B lymphocytes could exceed CD3~+ T lymphocyte subset after 7 days. CD8~+ cell is the main T lymphocyte subset in tonsil of appendix, and B lymphocyte is IgM~+ cell, and the amount could exceed CD3~+ T lymphocytes after 35 days. After 21 days, B lymphocytes in esophago tonsil are the main IgA~+ cells and the amount exceeds CD3~+ lymphocytes. The amount of CD4~+ lymphocytes is more than CD8+ lymphocytes.4. CD3~+、CD4~+ and CD8~+ T lymphocytes in spleen mainly distribute in periarterial lymphoid sheath. However IgM~+、IgG~+ and IgA~+ cells mainly distribute in ellipsoid periarterial lymphoid sheath and germinal center. T lymphocytes in appendix tonsil mainly distribute in middle and inferior part of mucous and the B lymphocytes mainly in middle and mucous between 4~7 days. Whereafter T, B lymphocytes equably distribute in mucous. CD4~+ cells arrange tightly and mainly occupy the central part in aggregates of T lymphocytes in esophago tonsil and CD8~+ lymphocytes mainly distribute in periphery. Meanwhile B lymphocytes encircle the periphery of aggregates of T lymphocytes. The aggregates of B lymphocytes is mainly the germinal center with lots of IgM~+、IgG~+ and IgA~+ cells. Meanwihle T lymphocytes encircle the periphery of aggregates of B lymphocytes.5. There is an intimate relationship between the development of tissue structure of peripheral immune organs and lymphcytopoiesis. The maturation of tissue structure is stimulated by the immigration of lymphocytes and the mature tissue structure provides place where lymphocytes grow mature and functionate.

脾脏在21日龄时达到稳定，食管扁桃体和盲肠扁桃体均在35日龄时达到稳定；脾脏中T淋巴细胞亚群以CD8~+细胞为主，B淋巴细胞则以IgG~+细胞为主，并在7日龄后数量超过CD3~+T淋巴细胞；盲肠扁桃体中T淋巴细胞亚群以CD8~+细胞为主，B淋巴细胞以IgM~+细胞为主，并在35日龄后数量超过CD3~+T淋巴细胞；21日龄后，食管扁桃体中B淋巴细胞以IgA~+细胞为主，数量超过CD3~+细胞，CD4~+细胞的数量多于CD8~+细胞。4、在T、B淋巴细胞组织定位方面，脾脏中CD3~+、CD4~+和CD8~+T淋巴细胞主要分布在动脉周围淋巴鞘中，而IgM~+、IgG~+和IgA~+细胞主要分布在椭球周围淋巴鞘和生发中心中；4～7日龄时，盲肠扁桃体中T淋巴细胞主要分布在粘膜固有层的中下部区域，而B淋巴细胞则主要分布在中上部区域，随后各日龄T、B淋巴细胞均匀地分布在粘膜固有层中；在食管扁桃体的T淋巴细胞聚集物中，CD4~+细胞紧密排列，主要占据中央部位，CD8~+细胞主要散布在外周，同时B淋巴细胞又环绕在整个T淋巴细胞聚集物的外周；B淋巴细胞聚集物主要为生发中心，其中存在大量IgM~+、IgG~+和IgA~+细胞，同时T淋巴细胞又环绕在整个B淋巴细胞聚集物的外周。5、外周免疫器官的组织结构发育和淋巴细胞发生之间存在密切的关系，淋巴细胞迁入淋巴器官刺激组织结构的发育成熟，同时成熟的组织结构又为淋巴细胞发育成熟并行使功能活动提供场所。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型，通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用；采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用；利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

The inoculation of MDV in general indued to decrease the production of IL-2、 IFN-γ-Th〓 cytokines in vitro, and there are differences between spleen and Thymus lymphocyte on the production of IL-2 in vitro, only thymus lymphocyte IL-2 prodution increased transient in early time. The cytotoxity TNF induced in thymus and spleen lymphocyte and bone marrow macrophage shown a simillar curve of"V"shape. MDV infection cause cell-mediated immune declines.

MDV接种总体上降低IL-2和IFN-γ-Th〓细胞因子体外诱生，其中IL-2体外诱生在胸腺细胞和脾淋巴细胞有差别，前者有早期短暂增强作用；而对TNF在上述两种细胞和骨髓巨噬细胞的诱生作用呈现类似规律，即呈现&V&形曲线，在接种后早期（1~5天）和25天升高，而在10天开始下降，至第20天降至低谷，MDV感染导致雏鸡细胞免疫水平下降，促进MD发病。

CD58, also was called lymphocyte function-associated antigen 3, is a surface glycoprotein expressed on many cell types, binds to the T lymphocyte surface glycoprotein CD2. This interaction is important in mediating T lymphocytes with target cells and antigen-presenting cells, and the T lymphocyte rosetting with erythrocytes. It plays an important role in mammal immune system.

CD58又称淋巴细胞功能相关抗原-3(Lymphocyte Function associated Antigen-3， LFA-3)，是存在于机体内多种细胞及血小板表面的一种糖蛋白，它可与CD2结合，从而介导T细胞与抗原递呈细胞等的黏附，此外，还可介导T细胞与绵羊红细胞形成Et花环，在机体免疫系统中具有重要作用。

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起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。