查询词典 liver

Augmenter of liver regeneration;DNA,recombinant;hepatocytes;cell proliferation;transaminases

肝再生增强因子； DNA ，重组；肝细胞；细胞增殖；转氨酶类

Effect of Augmenter of Liver Regeneration on Na(superscript +), K(superscript +)-ATPase Activity of HepG2 Cell

篇名 肝再生增强因子对HepG2细胞Na，K-ATP酶的影响

RESULTS: Recombinant human augmenter of liver regeneration could decrease ALT, AST, LDH concentration of fibrotic rats.

观察肝纤维化大鼠血清丙氨酸转氨酶、天冬氨酸转氨酶、乳酸脱氢酶水平，肝组织胶原蛋白含量及肝脏病理学改变。

Objective: To express rALR in GS115 with the constructed pichia pastoris expression plasmid of rat augmenter of liver regeneration and purify it with ultrafiltration and identify its bioactivity in vitro, for studying its biological functions.

目的：利用已构建的大鼠肝再生增强因子毕赤酵母重组表达质粒pPIC9K-rALR，在酵母菌GS115中诱导表达rALR，并进行纯化和体外生物学活性鉴定，为进一步研究rALR的生物学功能提供基础。

Objective To evaluate the effects of newborn rat hepatocyte intrasplenic transplantation combined with rat augmenter of liver regeneration injection in treating rats with acute hepatic failure. Methods Acute hepatic failure was induced in rats using D-gal (1.2 g/kg).

目的 探讨新生大鼠肝细胞脾内移植联合重组鼠肝再生增强因子治疗大鼠急性肝衰竭的疗效与免疫学机制。

Objective To investigate the protective effects of recombination rat augmenter of liver regeneration on tubular cell injury and renal dysfunction in rats with acute renal failureinduced by gentamicin.

作者单位：400010 重庆医科大学附属第二医院肾内科，重庆医科大学病毒性肝炎研究所

Coli JM109 expressing human augmenter of liver regenration.

摘 要：目的：研究表达重组人肝增强因子工程菌JMl09的发酵工艺。

MTT assay and -TdR incorporation were used to test the proliferation of HepG2 cell caused by augmenter of liver regeneration.Inorganic phosphorus spectrophotometer was used to test the activities of the Na~+,K~+ATPase.

采用四甲基噻唑盐法检验肝再生增强因子对H epG 2细胞增殖作用；[3H]-T dR掺入测定细胞DNA合成；采用无机磷比色法测定细胞N a+，K+-ATPase的酶活力。

Objective It was reported that rALR (rat augmenter of liver regeneration) distribute in most of tissue and organ of rat, no-tissue specility; mRNA expression of rALR was identified variedly in different tissue, strongest in testis. But nowadays there was not some study on ALR in rat testis. Here to confirm the expression and localization of ALR in infancy,adult and old rat testis,comparing the change of ALR expression degree before per 70 PH and after per 70 PH. In order to evaluate expression principle of ALR in rat testis and know if ALR has tight connection with spermatogenesis and development of testis, to explore the relation between ALR and reproduction, to discover the possible expression character and functional methods of ALR.

目的：文献报道rALR分布于各组织器官，无组织特异性，但其mRNA在各组织的表达强度有差异，在睾丸组织表达最强，目前对大鼠睾丸ALR表达尚无深入的研究；本实验研究不同年龄段大鼠睾丸组织中肝再生增强因子的表达水平、表达部位、以及表达的方式，进而探讨睾丸ALR与睾丸发育、精子生成以及生殖活动的关系；同时探究睾丸组织ALR表达是否参与了肝脏的再生过程中，进一步了解睾丸ALR表达的特性及作用方式。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。