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The uncovered tracts and the liver wound surface were managed by some of the methods combined such as high frequency electrocoagulation, titanium clamp, suture, hemostatic gauze oppression, argon-beam coagulation, protein glue adherence, and Endo-GIA switcher. The total operative outcome, operating time, blood loss, postoperative complications and postoperative hospital stay were summarized in order to assess feasibility and safety of the clinical laparoscopic liver resection.Results: 1. Laparoscopic liver resection with clamp dissection method needs only the ordinary laparoscopic equipment. It has a low cost but causes a little more bleeding. 2. Laparoscopic liver resection operated by microwave tissue coagulation method had very little bleeding when dissecting hepatic parenchyma. The operative field is clear and the cost is low. It is a safe, convenient and effective method. 3. Ultrasound dissector is a relatively ideal appliance for laparoscopic liver resection at present because ofless bleeding during operation. But the speed is slow thus prolongs the operation time. Its separating intensity is not strong enough, and it can only be applied to patients without hepatic cirrhosis. 4. Ligsure has clamp dissection and electrocoagulation functions. It can carbonize hepatic tissue including the tracts whose diameters are less than 7mm. It has some advantage in dissecting. However, it has the shortcomings of the clamp dissector during coagulating. 5. Hand-assisted laparoscopic liver resection can make use of the flexibility and feeling of the operators left hand.

结果:1、钳夹分离切肝法不需特殊仪器设备,只要具备常规的腹腔镜器械即可实施,成本低,肝断面较易渗血;2、微波固化切肝法肝实质出血很少,视野清晰,成本低,操作简单,是一种安全、简便、有效的方法;3、超声刀是目前较为理想的切肝器械,术中出血少,但是,切割速度慢,手术时间长,而且,切割强度有限,适用于无肝硬化的肝切除;4、Ligsure具有挤压粉碎及高频电凝止血两大功能,可使包括7~以下管道的肝组织碳化、形成焦痴,具有一定的优势,但是,也存在分离钳电凝止血的缺点;5、手辅助腹腔镜肝切除可以利用术者左手的灵活性及手感,便于术中显露、分离、控制出血等,可随意协助右手及助手的主要操作,缩短了手术时间,大大提高了腹腔镜肝切除的安全性,李朝龙等的改良方法,同样达到了手辅助目的,又节省了费用;6、小切口腹腔镜辅助切肝法可以使用剖腹肝切除常规器械及剖腹肝切除技术,操作简单、可靠,适用于肝左外叶切除及右肝第V段切除;7、Endo一GIA切肝法在离断肝组织的同时闭合管道结构,多用于肝左静脉、门静脉分支、管径较大胆管的切割,缺点是不能用于较厚肝组织的切割,而且价格昂贵;8、临床巧例腹腔镜肝切除均获得成功,手术时间最短1.5h,最长sh,平均125 min,多数在100 min左右,手术出血量最少50 ml,最多500 ml,平均1 78 ml,除2例合并严重肝硬化的原发性肝癌患者术后出现少量腹水,1例术后发生胆漏外,其余无并发症,发生胆漏的1例患者术后住院40d,多数在术后一周左右出院,术后住院时间5一40d,平均gd。

In an effort to develop a simple method for identifying patients who may have fatty liver disease, Dr. Bedogni and colleagues used data from the Dionysos Nutrition and Liver Study to determine the degree to which drinking habits, anthropometry, and metabolic parameters contribute to the risk for the condition in 216 patients with suspected liver disease but no hepatitis B and C infection as well as in 280 age- and sex-matched controls. All participants were from the same town in northern Italy. The presence of fatty liver disease was confirmed via ultrasonography. Results were presented here by Vittorio di Maso, MD, another researcher at the Liver Research Center, at the 41st annual meeting of the European Association for the Study of the Liver.

为了发展出一个简单的方法於鉴定脂肪肝疾病病患,Bedogni 医师及其同僚使用迪奥尼修斯营养与肝脏研究的数据,对280位年龄与性别相符者之中的216位疑似有肝脏疾病但无B型或C型肝炎的病患,加以区别饮酒习惯、人体测量学、导致风险的代谢参数等,所有参与实验者都是来自义大利北部的一个城镇,藉由超音波扫瞄确定出现脂肪肝疾病;研究结果在欧洲肝脏研究学会第41届年会由前述肝脏研究中心的另一位研究员、Vittorio di Maso 医师发表。

The method uses tall fat emulsion to build bandicoot tall lipemia model of sexual fatty liver, experimental cent is group of high dose of drink of fat of regular set, model group, disappear and small dose group, treasure liver peaceful is electropositive group to the south of, with drink of fat of disappear of tall, small dose peaceful of He Dongbao liver undertakes intervention, determine branch content exceeds to cross oxide in liver fat content and liver enzymatic active, third 2 aldehyde content and vitamin E level.

方法采用高脂乳剂建立大鼠高脂血症性脂肪肝模型,实验分为正常组、模型组、消脂饮高剂量组和低剂量组,以东宝肝泰为阳性组,以高、低剂量消脂饮和东宝肝泰进行干预,测定肝脂含量及肝中超歧物过氧化物酶活性、丙二醛含量和维生素E水平。

The coat is liver and white, usually either liver and white spotted, liver roan, liver and white spotted with ticking and roaning or solid liver.

颜色 被毛颜色为肝色和白色,通常,肝色或白色斑点,肝色杂毛,肝色或白色斑点在纯肝色或肝色杂色上,都可以。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤,对其中10株进行克隆化,获得bFGF特异单抗16株,2株针对融合蛋白;应用该单抗建立了0.1ng/孔灵敏度的ELISA,0.5ng/孔敏度的斑点ELISA;用Western-blotting鉴别了经改造不同工程菌蛋白表达,指导重组工程菌改造;用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点;合作研究了20例肝癌、肝癌细胞株和正常肝组织,发现前者bFGF高表达;应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF,纯度达到95%;发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体,已有10多家单位引用单抗或进行合作。

objective to search the factors of liver injury via the anit-induced liver injury in rats.methods male sprague-dawley rats,were divided into 4 groups,data were analyzed using a completely randmized factorial anova and linear correlation.results after anit intoxication,the necrosis of hepatic cell and neutrophil infiltration im periportal region of the liver were observed in liver setions.jinshisan can reduce the alt,the tbil.pla2 correlated positively with alt、akp and tbil.conclusion the severity of anit-induced liver injury correlated positively with the pla2.jinshisan can protect against the rat anit-induced liver injury.

目的 拟通过对α-萘异硫氰酸酯诱导大鼠肝损伤发病机理的探讨,对引起肝损伤的因素进行系统的研究。方法选择s-d大鼠,雄性,随机分4组,检测数据以平均值±标准差表示,采用方差分析,相关分析进行统计学处理。结果s-d大鼠经anit染毒后,病理切片显示肝损伤明显,汇管区有大量中性粒细胞浸润。经金石散及地塞米松治疗后,生化指标均有所下降,中药金石散优于地塞米松,血生化指标与pla2活性相关分析显示,呈正相关。结论 anit诱导的肝毒性损伤程度与肝组织匀浆pla2活力呈正相关,应用抑制剂降低pla2活力,肝损伤程度也减轻,中药金石散具有降低pla2活性,减轻肝毒性损伤。

The indications were: acute liver failure in one (4%); chronic liver failure in 25 (96%)-10 metabolic, six biliary atresia, five polycystic/liver fibrosis, four other, and one retransplant. Nine (35%) received partial graft; 5 (19%) multivisceral grafts (liver-kidney, liver-bowel) and 12 (46%), conventional OLT.

适应症:急性肝功能衰竭1例( 4 %);慢性肝功能衰竭25例( 96 %),其中10例代谢病,6例胆道闭锁,6例多囊肝/肝纤维化,其他4例中一个为再次移植。9个( 35 %)接受部分肝移植; 5个(19 %)接受多脏器移植和12个(46 %)接受常规肝移植。

Conservative treatment, debridement and suturing and deep mattress suture were taken for I-II degree liver trauma, greater omentum or gelatin tamponage plus suture were chosen for III degree liver injury, debridement with partial hepatectomy was chosen for IV degree liver injury and gauze tamponage and infeior vena cave in posterior liver repairing resection was performed depending on position of liver injuries and general conditions of the patients. Results showed that the total cue rate was 94.2%.

级肝损伤采用保守治疗、单纯清创缝合和深部褥式缝合的方法,Ⅲ级损伤选择大网膜或明胶海棉填塞加缝合的术式,Ⅳ级损伤行清创性肝切除术,Ⅴ级损伤则根据患者损伤的部位及全身情况采用单纯纱布填塞和肝后下腔静脉修补术,结果治愈率达94.2%。

PartⅢ: Normal liver regeneration after partial liver radiation: an experimental study onrats.Objective: To verify whether unirradiated normal liver has the ability to regenerateafter partial liver irradiation, and to investigate the timing of liver regeneration and the rolesof participated cytokines through an experimental study on rats.

第三部分:大鼠部分肝照射后正常肝脏再生的实验研究目的:通过对大鼠进行部分肝脏照射,检测未受照射的正常肝脏肝细胞是否出现再生现象,探讨再生的时间以及参与的细胞因子的作用。

The therapy of protection of liver function is very necessary: diammonium glycyrrhizinate can recover the non-specific damage of liver cells, Fufangdanshen can improve the microcirculation of liver, and tiopronin and reduced glutathione has the unique function to recover liver damage caused by fat invasion. Of 168 cases, 60 cases (35.7%) show exacerbation of liver function after using fat-reducing drugs for 1-2 weeks. Of 41 cases (male 36 and female 5) with the complication DM2, 58.5%(24/41) of cases also show exacerbation of liver function after using sugar-reducing drugs.

保肝治疗非常必要,甘草酸二铵有修复非特异性的肝细胞损伤的作用,复方丹参可以改善肝脏微循环;硫普罗宁或还原性谷胱甘肽,对于脂肪肝之由于脂肪浸润引起的肝细胞损伤的修复有独到的作用。168例中有60例(35.7%)因用降脂药物1~2周出现肝功能异常的加重;合并2型糖尿病的41例(男36例,女5例)患者中有58.5%(24/41)的人用降糖药物后也出现肝功能异常的加重。

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