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A method based on liquid phase microextraction with gas chromatography-flame ionization detector was established for the determination of aminopyrine,antipyrine and barbital in Urine.

建立了一种以液相微萃取(Liquid Phase Microextraction，LPME)与气相色谱-氢火焰(LPME-GC-FID)联用技术为基础的测定尿样中氨基比林、安替比林和巴比妥的方法。

When proceeding the research of drug identification by ultraviolet spectrophotometry, this paper adopts the approaches below: to identify Metronidazole Buccal Tablets and Potassium Sodium Dehydroandroan Drographolide Succinate by ulviolet maximum absorbent wavelength; to identify Metronidazole Disodium Phosphate for Injection by ulviolet maximum absorbent wavelength and minimum absorbent wavelength; to identify Ceftriaxone Sodium for Injection and Clarithromycin Tablets by retention time of high performance liquid chromatography- ultraviolet spectrophotometry.

通过测定紫外最大吸收波长λ_(max鉴别甲硝唑口颊片和炎琥宁、测定紫外最大吸收波长λ_(max和最小吸收波长λ_(min鉴别注射用甲硝唑磷酸二钠，结合高效液相色谱(high performance liquid chromatography-ultraviolet detector，HPLC-UV)中保留时间的测定鉴别注射用头孢曲松钠和克拉霉素片，本文建立了药物鉴别的紫外分光光度法。

Methods: Determining paeoniflorin content by high performance liquid chromatography measurement conditions: mobile phase of acetonitrile -0.4% phosphoric acid solution (volume ratio 13∶87; detection wavelength 230 nm; flow rate 1.0 mL/min; and column temperature 25℃; and determining polysaccharide content by anthrone-sulfuric acid method. The extraction procedure of Langchuangling granule was measured, and the evaluated indexes were the rate of dry extra, contents of the paeoniflorin and the total polysaccharide with L9 (34) orthogonal test and preferential treatment of data.

采用高效液相色谱（high performance liquid chromatography，HPLC）法测定芍药苷含量［测定条件：流动相为乙腈-0.4%的磷酸水溶液（体积比为13∶87）；检测波长230 nm；流速1.0 mL/min；柱温25℃］；采用硫酸-蒽酮法测定总多糖含量，并以干浸膏得率、总多糖含量和芍药苷含量为指标，采用L9（34）正交实验，用综合评分法处理数据优选水提取工艺。

This thesis demonstrates the pre-polymers in a polymer-dispersed cholesteric liquid crystals can be polymerized into a polymer film, which can eliminate the aligning capability of the homogeneous alignment layer. Thereafter, the textures of the cholesteric liquid crystals in UV-cured region can be changed from reflective planar texture to the scattered focal conic one. Additionally, the clearing temperature of the liquid crystals can be reduced by doping with chiral dopant or monomers. The cause is the increase of the impurity concentration in LCs. In other words, if the monomers are polymerized, the clearing temperature of LCs in that region should increase due to the decrease of the impurity concentration. Therefore, impurities associated with doping with monomeric and chiral dopants cause the clearing temperature of LCs in cured region to differ from that in uncured region.

本文中，我们提出在高分子聚合物薄膜之胆固醇液晶(polymer-dispersed cholesteric liquid crystals，简称PDCLCs)中，利用紫外光使PDCLC中的单体聚合成薄膜，此即为光引致聚合反应，而该薄膜将原本在表面所作的配向膜覆盖且破坏其配向能力，使胆固醇液晶由原本反射的planar结构转换成散射的focal conic结构；另外，有关液晶材料的相变温度会由於掺杂手性分子或单体而降低，此因液晶材料内『杂质』浓度增加，而造成的液晶材料之不纯度增加，最终导致相变温度降低，若搭配上述光引致聚合反应，则由於光照区之单体聚合，使该区域单体占总材料之比例下降，即不纯度降低，进而使液晶材料之相变温度上升，该区域之相变温度与未照光区有所不同。

Methods: The content of gallic acid in Duoxuekang was determined by high performance liquid chromatography.

采用高效液相色谱法（high performance liquid chromatography，HPLC）测定多血康中没食子酸的含量。

John Conrad's expert research group, following the research of world enamel authority Dr. Conrad. After seven years of diligent research in Taiwan, China, and the U.S.A., he finally uncovered the secret of Song Dynasty Jian Kiln black glaze, reproducing the peerless style of liquid in liquid separated glazes. It can be said that he is a pioneer and a revivalist of contemporary ceramics, and he is generous with his glaze formulas, answering any questions. This has cemented his status as a ceramics master and a transmitter of Chinese traditional ceramics culture.

康纳德（ Dr。 John Conrad ）专家研究组召集人，跟随世界釉药权威康纳德博士进行研究，历经七年在台湾、中国、美国三地努力地研究后，终於在1995年揭开宋代建窑黑釉的秘密，重现「液液分相釉」（ Liquid in liquid separated glazes ）的绝代风华，可说是当代陶艺研究先驱与再创者，而其不藏釉料秘方有问有答的态度，更加强了他陶艺大师与中华传统陶艺文化薪传者的地位。

For it is rubor after damaging to the closing soft tissue, getting swollen, getting hot aching and model of functiolaesa these bed solicit and there is no fracture,no skin damaged, we designed the corresponding experiment, namely the law of hot board causes little mouse\'s painful experiment of threshold of pain; Freun\'s complete adjuvant caused the swollen experiment of big mouse\'s foot;The xylene caused the swollen experiment of little mouse\'s ear; Invigorating blood circulation silt experiment that changes the sex that the rheology appearance examines the blood and flows with the blood; Useing the high performance liquid chromatography-electrochemistry Detected monoamine neurotransmitters transmitter in the brain and serum; the experiment of immunohistochemistry measured the proto-oncogene C-FOS expresses.

针对闭合性软组织损伤后的红、肿、热、痛及功能障碍的典型临床体征，加上无骨折、无皮肤破损的特点，我们设计了相应的实验，即热板法致小鼠疼痛的痛阈实验；福氏完全佐剂(Freun\'s complete adjuvant，FCA)致大鼠足肿实验；二甲苯致小鼠耳肿实验；用血流变仪测血液流变性的活血化瘀实验；运用高效液相色谱—电化学(high performance liquid chromatography-electrochemistry Detection HPLC-ECD)法测量大脑及血清中单胺类神经递质的实验以及免疫组织化学法检测原癌基因C-FOS蛋白产物表达的实验。

They include the variation of the degradement velocity of different sample, the inhibition product of PCR, the amount of DNA extraction, quality of DNA and the heteroplasmy of some tissue. Correct sequencing, comparison of the result of forward and reverse primers sequencing should be done to rule out wrong signals, and prevent contamination. In addition to gene sequencing, low grade heteroplasmy could be dealt by Denaturing high performance liquid chromatography. Multiplex Ligation-dependent Probe Amplification. The analysis of mitochondrial HV Ⅰ, HV Ⅱ sequencing is able to identify the maternal sanguinity, then it could assist in the human identification. It also complements the genomic STR system in distribution of population, the origin of human, and analysis for fossil.

分析粒线体DNA时必须考虑各类检体腐败DNA降解速度之不同、PCR的抑制物质、DNA的萃取量及品质、特定组织的异质现象、萤光序列分析图及正、反向引子定序结果比对、排除杂讯干扰、防治污染等，除以基因定序外，低程度的异质现象也可用Denaturing high performance liquid chromatography、Multiplex Ligation-dependent Probe Amplification等方法分析，粒线体HV Ⅰ、HV Ⅱ碱基序列的分析可鉴别一个人的母系血缘关系、辅助人类身分鉴定，对於族群分布、人类起源探讨、远古检体的分析可作为辅助STR系统的利器。

In this study, isobaric tags for relative and absolute quantification labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) was employed to compare the differential protein expressions in MCF7 cells upon 17β-oestradiol (E2) stimulation or serum deprivation versus in normal conditions. A total of 576 proteins with the confidential level of ≥95% were identified, 26 proteins among the 576 proteins were found to be differentially expressed after E2 stimulation or serum deprivation, and among them 10 proteins were up-regulated and 16 proteins were down-regulated by more than two folds.

利用四重用于相对和绝对定量的等量异位标签(isobaric tags for relative and absolute quantification， iTRAQ)标记结合二维液相色谱-串联质谱(two-dimensional liquid chromatography-tandem mass spectrometry， 2D-LC-MS/MS)对雌二醇(17β-oestradiol， E2)和血清各自以及共同作用对MCF7乳腺癌细胞内蛋白质表达的影响进行了比较，共鉴定到置信度在95%以上的蛋白质576种，其中各组相对于正常培养组的细胞共找到26种差异在1倍以上的蛋白质，其中10种上调，16种下调。

Based on constant altitude plan data like reflectivity factor, collected by a new weather radar interpolated with 3D-Barnes scheme, in this paper the vertically integrated liquid water content and vertically integrated liquid water content density of hailstone cells are calculated with the theoretical model, ration of VIL and upright height of hailstone cells, and the maximums of VIL and VILD are identified using MAX function.

以3D-Barnes方案插值的新一代天气雷达反射率因子等高平面资料，用垂直累积液态含水量（vertically in-tegrated liquid water content，简称VIL）的理论模式计算单体的VIL，用VIL与单体顶高度之比计算单体的垂直累积液态含水量密度（vertically integrated liquid water content density，简称VILD），利用MAX函数逐个提取雹云单体在降雹过程中的最大VIL和VILD（分别简称VILmax和VILDmax），采用统计和回归处理技术，利用2004-2006年的5~8月甘肃中部54例局地冰雹单体个例，对单体VILmax和VILDmax与地面最大降雹区的位置、地面最大冰雹直径之间的相关性以及对Rmax的识别效果等进行了对比分析。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。