查询词典 liposome

So the REV was fixed as the preparation method of PST liposome in this article; and the protamine aggregation was also fixed as the determining method of the rate of encapsulation of the product.

因此确定了逆相蒸发法为本文PST脂质体的制备方法；鱼精蛋白法为试验产品包封率的测定方法。

Methods: cationic liposome lipofectamine2000 was used to transfect retrovirus vector pbabe pten puro containing pten and pbabe puro into pten deficient prostate cancer cell line pc 3 respectively, and then cell growth, adhesion, segregation, apoptosis, cell cycle and ultrastructure alteration in vitro were studied.

利用阳离子脂质体lipofectamine 2000介导，分别将含pten的逆转录病毒载体pbabe pten puro及空载体pbabe puro转入pc 3细胞，观察转染前后细胞的生长、粘附、超微结构、细胞周期改变情况。

Use the magnetic material as carrier to prepare Semustine Magnetic Liposome, the average particle diameter of it is 184.5 nm. It contains the chemical reconstituent of Fe3O4, the drug envelopment rate is 73.72%.

以此磁性材料为载体制成的司莫司汀磁性脂质体的平均粒径为184.5 nm，其中含有Fe3O4成份，药物包封率达到73.72%。

Recently the liposome reconstitution technique has been introduced to the field of botany,used for the study of plant membrane proteins.

近年来脂质体重组技术开始引入到植物学研究领域，用于对植物膜蛋白的研究。

Recently the liposome reconstitution technique has been introduced to the field of botany, used for the study of plant membrane proteins.

近年来脂质体重组技术开始引入到植物学研究领域，用於对植物膜蛋白的研究。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

And mices'embryos of various ages were gotten and 81 of 793 embryos had fluorescence. Secondly, we directly injected the gene/ liposome complex into 5 male mice's spermaduct and 75 of 810 embryos had fluorescence.

其中睾丸注射法、输精管注射法和外源基因/精子共孵育法分别各处理公鼠5只，7d后各配母鼠25只，结果分别冲出胚胎793枚、810枚和357枚，荧光显微镜下检测到荧光的分别有81枚胚胎(10.2%)、75枚胚胎(9.26%)和53枚胚胎(14.9%)。

Objective To synthesize new cationic liposome cytofectin N 4 spermine cholesteryl carbamate as a gene delivery vehicle for gene therapy of lung diseases.

目的 合成新型阳离子脂质体细胞转染素N4 精氨胆固醇羰酰氨，作为基因载体，用于肺部疾病的基因治疗。

Methods: Lecithin and the sucrose ester-phospholipids liposome were prepared using the ethyl ether injection method by mixing sucrose ester with lecithin, sphingomyelin and cholesterol according to different lipids ratio.

按不同比例将乙酸异丁酸蔗糖酯与卵磷脂、鞘磷脂和胆固醇混合，溶于乙醚中，然后注入pH7.2的PBS缓冲液中，制成卵磷脂脂质体及蔗糖酯－卵磷脂脂质体。

Methods: The NGF purified from mouse submaxillary glands was packed with liposome and prepared into freeze dying agent.

将纯化得到的小鼠颌下腺的NGF用脂质体包裹后分别制成冻干剂型。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。