查询词典 liposome

In this paper, the perturbations of CTX-IX, which is a kind of cardiotoxin isolated from Naja naja atra, on artificial liposome, reconstituted system of dellpidated ATPase and phospholipld and mouse lymphacyte were investigated in order to understand the membrane toxlcology of CTX-IX in view of molecular dynamics of membrane, give clues for searching new toxic polypeptides that could kill the harmful cells and establish a example for the study of lipld-protein interaction and corresponding significance in membrane function.

本文研究了广东中华眼镜蛇毒中分离得到的心脏毒素CTX-IX和人工磷脂水化聚集体、ATP酶-磷脂重组体系和小鼠脾细胞之间的相互作用，试图从膜分子动力学角度理解心脏毒素的膜毒理，为杀伤细菌等有害细胞的毒性肽的寻找提供线索，在理论上亦为脂蛋白相互作用与膜结构功能的关系研究提供一个范例。

The therapy groups displayed significant improvements in neuromotor functions and survivals of neuron and oligodendrocyte compared with control groups. These results demonstrated the neuroprotect roles of GGF2, and cationic liposome-mediated GGF2 transgene therapy has potential possibility.

结果：1、大鼠LFP致伤后3—7天，GGF2 mRNA在海马CAl区、CA2区、CA3区、DG及皮层表达明显增加。2、阳离子脂质体介导GGF2转基因治疗可促进大鼠伤后行为学的恢复，可促进损伤区神经元及少突胶质细胞的存活。3、原核表达方法得到GGF2重组蛋白。

It was showed that liposome was safe formulation and was nonirritating. The biological stability was also tested by means of

对NGF脂质体的安全性进行了考察，家兔耳血管刺激性实验、滴眼刺激性实验及溶血实验结果表明该制剂的刺激性小；小鼠异常毒性实验

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Objective To explore the best formula of the 0 5% liposome podophyllotoxin chitosan film.

目的 探讨 0 5 %脂质体鬼臼毒素壳聚糖膜剂制备的最佳配方。

The period of validity of the podophyllotoxin liposome was forecasted in the condition of 4℃.

鬼臼毒素脂质体的稳定性与温度有关，温度越高，稳定性越差，应低温保存。

Purpose To estimate the stability of podophyllotoxin liposome.

目的 预测鬼臼毒素脂质体的稳定性。

Samples were taken at certain time intervals and the content of podophyllotoxin liposome was determined using HPLC.

结果 在光照条件下放置10d的K值较0d增加的最多，增加了12.21倍，而在4，25，30，40℃放置10d的KE值较0d分别增加了3.55，5.85，6.96，8.12倍；鬼臼毒素脂质体在4℃下的有效期t0.9为5d， t0.5为60d。

By using liposome-mediated gene transfection technology, plasmid pCMVSV40T/PUR containing SV40Tag was transfected in primary embryonic precartilaginous stem cells, while non-transfected cells served as negative controls.

利用脂质体介导基因转染技术将含有SV40Tag的质粒pCMVSV40T/PUR转染原代胚胎前软骨干细胞，以未转染细胞作阴性对照。

Objective Fluorescent-labeled antisense oligomer that is hybridized to 18-bp sequences next to the start coden of human proliferating cell nucleus antigen gene was used to assess the intracellular distribution and stability of the oligonucleotides in the presence or absence of liposome.

目的 观察硫代修饰型及天然型PCNA反义寡核苷酸在脂质体介导下或直接转染人膀胱癌细胞BIU-87后在细胞内的分布及其稳定性。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。