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In this study, the structure sequences of Shiga toxin 2B(stx2B) and Shiga toxin 1B(stx1B) were respectively amplified from EHEC O157:H7 by PCR. stx2B-L-stx1B fusion gene was constructed by a 12 bp linker ligating these two fragments. After digested with restriction endonuclease Nco Ⅰ and EcoR Ⅰ, the fusion gene was orientally inserted into expression vector pET28a.

利用PCR方法，从肠出血性大肠杆菌O157：H7的基因组DNA中，分别扩增出Stx1B和Stx2B亚基的结构基因片段，然后再通过PCR将这2个片段连接起来，并定向克隆到表达质粒pET28a的NcoⅠ和EcoRⅠ位点之间，构建了重组表达质粒pMB1。

Libtool is a command line interface to the compiler and linker that makes it easy to portably generate static and shared libraries, regardless of the platform it is running on.

Libtool是一个基于命令行的接口，通过该接口可以和编译器和链接器打交道，从而可以很容易的可移植性的编译出静态或者共享库，无论它在什么平台下面运行。

The result shows that the temperature for copolymerization, the dosages of initiator and cross-linker are major factors while the amount of dispersing agent and rotative velocity of agitator are minor factors affecting the absorbility.

结果表明聚合温度、引发剂浓度和交联剂用量是决定吸水率和吸盐水率的主要因素，而分散剂用量、搅拌转速对反应结果影响不明显。

Because ouabain polyclonal antibody made blood pressure reduced for shorter time and possessed slightly allergy, we aimed to prepare a new depressor, ScFv, with high specificness by advanced phage display technique. The heavy-chain and kappa light-chain variable region gene repertoir of immunoglobin were amplified individually with heavy chain and light chain primers of Pharmacia Corporation and assembled into ScFv by a linker with overlap extension PCR.

针对哇巴因多克隆抗体存在的降压时间持续较短及存在轻微的过敏反应等缺点，本研究利用先进的噬菌体抗体库技术，拟研制开发一种具有高度选择性的降压新药--哇巴因单链抗体。

There are chiefly two models explaining the S4 movement: the helical screw (S4 move translationally and rotationally in a gating canal which is composed of part of the channel protein), and the paddle model (S4 lying at the channel periphery and moving as one unit with S3b).

本文即藉由S3与S4间之连接段(S3-4 linker)和S4本身之非碱性胺基酸的突变，来探讨S4在去极化时移动的方式与距离，以及包围在S4外侧之主要电阻区的环境与位置。

It is possible that the linker proteins located in the center of APC discs may play a important role in stabilizing this loose APC hexamer.

有可能位于APC盘中心的连接蛋白对稳定这个APC疏松的六聚体起重要作用。

Genomic DNA in eukaryotic cell is organized into nucleosome array and further packaged into higher-order structures. Each nucleosome is composed of a stretch of approximately 147bp DNA wrapping around a histone octamer, and neighbored nucleosomes are connected by linker DNA with length varies from 20bp to 100 bp.

中文摘要真核生物的基因组DNA能够与组蛋白形成核小体结构，这些核小体结构在基因组上线形排列，并进一步折叠成更加紧密和复杂的高级结构。

When the linker builds your module, it embeds a relocation section in the resulting file.

当链接器生成的模块，它内嵌了在生成的文件迁移部分。

The loading method and the quantity of linker had greater effect on in-vitro release curve. Release curve of incubation-absorption and entrapping method had two phases—quick release phase and slow release phase. And the first phase lasted about one hour and released 70% drug.

载药方法和交联剂用量对释药曲线有显著影响，吸附法和包埋法制备的载药DSMs的释药曲线明显分为快速释药阶段和缓慢释药阶段，第一阶段持续约1 h，释放药物的70%左右。

The results show that the linker length has no influence on the intercalating activities and the nitro-containing parent compound has strong DNA-cleaving activities.

通过对DNA嵌入常数Ka和切断活性的测定，观察到连接臂长短对嵌入作用没有明显影响，含硝基化合物具有较强的DNA切断作用。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。