查询词典 lines

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Existing large-scale enterprises glauberite ore two domestic advanced production lines of four sets of Sodium Sulfate, Sodium six production lines, two sets of alkaline protease production lines, pharmaceutical production lines mirabilite set.

企业现有大型钙芒硝矿两座，国内先进的元明粉生产线四套，硫化钠生产线六套，碱性蛋白酶生产线两套，药用芒硝生产线一套。

The Company is comprised of six business divisions: Biscuit, whose product lines are Roma, Better, Prima and Danisa; Candy, whose product lines are Kopiko, Kis, Tamarin and Plonk; Wafer, whose product lines are Beng Beng, Sando, Astor and Roma; Chocolate, whose product lines are Choki Choki and Danisa; Health Food, which offers the Energen product line, and Coffee, which offers the Torabika product line.

该公司是由六个业务部门：饼干，其产品线是罗姆人，更好，厦华和Danisa ；糖果，其生产线是Kopiko ， KIS专业版，万达和普朗克；晶圆，其产品线的崩崩，山东，阿斯特和罗姆人；巧克力，其生产线是Choki Choki和Danisa ；健康食品，它提供的Energen产品线，与咖啡，它提供的Torabika生产线。

A detection method for welding lines based on K-L algorithm is discussed. Infrared light source is applied to get high quality images of welding lines through CCD. Image template libraries for unwelded and welded lines are constructed and the welding line image is processed by K-L algorithm to match with the template libraries to judge if the welding line has been welded. The experiment shows that above method is effective with high precision and speed and it can be applied on real-time detection of welding lines.

提出计算机对所得图像进行滤波和图像分割，讨论了K-L算法的原理及其在红外焊缝图像检测中的应用；以红外光源照射焊缝，通过CCD摄取焊缝的图像，建立已焊和未焊的图像模板库，采用K-L算法对焊缝图像进行处理，并与模板库进行比较，得出焊缝是否焊接的结论；试验结果表明，该方法检测精度高，检测速度快，可以实时运用于对手工电弧焊的焊缝质量检测中。

Through the point views of macroscopical network and microcosmic lines, this article filtrates the guide lines of reasonable, then use clustering analysis method to analyze the bus network and lines. On base of this, through summarizing the practice experience of specialties layout for bus network, it gives out a idea and practical method of the analysis for bus lines'optimization way.

本文从宏观层面的公交线网和微观层面的公交线路两个角度筛选合理、易得的指标，用聚类分析方法进行分析，再以此为基础，通过对公交线网专项规划项目的实践经验的总结，提出了线路优化方式分析的思路和实用方法。

In recent years, the company has successfully developed some special production lines for manufacturing ordinary batteries, namely, D size, Size C, and Size AA. Now running in the workshop is another assembly line for carbon rod processing machinery. For the past decade, it has introduced most advanced manufacturing techniques and control techniques from abroad, and therefore it has managed to develop automated machines for producing alkaline batteries. The automatic assembly lines that it possesses are: Lines LR6 and LR03 for 200 cells per minute, 300 cells per minute, 400 cells per minute, 600 cells per minute and 1000 cells per minute, Lines LR20, LR14 and LR61 for 150 cells per minute and 300 cells per minute. Powder spraying conducting film, machines for negative electrode calamine cream, diaphragm machines with a speed of 30 cells per minute; negative electrode electric welding machines with a speed of 200-300 cells per minute; current collector assembling machines with a speed of 200-400 cells per minute; battery tray fillers with a speed of 200-400 cells per minute, insulating ring assembling machines with a speed of 300 cells per minute; electroscope machines, 4-cell furling machines with a speed of 300 cells per minute.

公司先后开发了普通电池生产线：一号、二号、五号电池流水线和普通碳棒加工机械，特别是近十年来公司吸收并消化了国外最先进的生产工艺、控制技术，研制开发了国内最先进的自动化碱性电池机械，有200只/分钟、300只/分钟、400只/分钟、600只/分钟、1000只/分钟的LR6、LR03电池自动化生产流水线；有150只/分钟、300只/分钟的LR20、LR14、LR61电池生产线；有正极拌粉系统喷导电膜设备、负极拌锌膏；有30只/分钟的卷隔膜套机，200-300只/分钟负极钉高速点焊机，200-400只/分钟的集电体高速组装机，200-400只/分钟的电池装盘机，300只/分钟的绝缘圈组装机，300只/分钟的验电机和四节缩机等。

Lodicules of the CMS lines with lighter CGG had more, bigger and unequal tracheae in SVB, and had bigger volume, heavier weight and lower water potential compared with the CMS lines with CGG. Similar to CMS lines, the B-lines had similar changes.

与种子裂颖重的不育系相比，种子裂颖轻的不育系浆片小维管束导管面积大、数目多且分布均匀、体积小、重量轻、水势低；且相应保持系也表现出大致相同的规律。

Molecular iodine has several hundred strong absorption lines near 532nm,several absorption lines were observed by using single-frequeny Nd:YVO4/KTP laser. There are new absorption lines which are not included in the nine groups recommended by CCDM in 1997.The wavelengths of these lines have been measured and the frequency stabilization investigated.

碘分子在532nm附近有上百条强的吸收谱线，采用单频Nd：YVO4／KTP激光对碘的一些吸收谱线作了观测，并进行了激光频率稳定的研究，这些吸收谱线是1997年9月国际米定义咨询委员会推荐作为频标的9组532nm碘吸收谱线之外的新的参考谱线。

Lines of tunable output are obtained, the energy of 18 lines is more than 10 J. In the rapidly trigging experiment, the tunable outputs of two random lines among the whole CO2 laser spectrum and two adjacent lines in the same transition band are realized respectively in 60 ms and 20 ms.

实验共得到85条调谐输出谱线，单脉冲能量超过10 J的有18条谱线；在动态快速触发情况下，分别在60 ms和20 ms内实现了整个CO2激光光谱范围任意两条谱线和同一个跃迁带内相邻两条谱线的快速调谐输出。

The next development in musical notation were "heighted neumes", in which neumes were carefully placed at different heights in relation to each other. This allowed the neumes to roughly indicate the size of a given interval as well as the direction. This quickly led to one or two lines, each representing a particular note, being place on the music with all the neumes relating back to them. At first these lines had no particular meaning and instead had a letter placed at the beginning indicating which note was represented. However, slowly the lines indicating middle C, and the F a fifth below, became most common. Having at first been merely scratched on the parchment, the lines now were drawn in two different colorded ink: usually red for F, and yellow or green for C.

音乐记谱法的再一次新发展是heighted neumes，这种记谱法标识出音符之间的音高关系，这样就使得纽姆谱可以粗略并直接地标记出指定音程的增减，这样的标识方法导致了谱上一线或者二线的出现，每条线代表一个特定的音符，这个音符与纽姆谱中所有的音符的音程关系都能被标识出来，最初这些线并没有什么特殊的含义，反而会有一个字母被放在开始的位置表明代表哪个音符，渐渐的，线代表中央C或者比C低五度的F 变得最常用，这些一开始只不过在羊皮纸上刮出的线，开始用两种不同的颜色书写，红色代表F，黄线或者绿线代表C，以上的这些便是我们所知道的音乐记谱法发展的开端。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷