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Guangdong college of pharmacy,guangzhou,guangdong 510006,china;henan university of traditional chinese medicine,zhengzhou,henan 450008,chinaabstract:objective to establish a method for the determination of the content of total alkaloid in nao mai tong recipe.methods acid dye colorimetry was used in the determination with bromocresol green buffer solution as the color developing agent with absorbance was determined at the detection wavelength of 417 nm.results the calibration curve of the absorbance was linear with the concentration of ligustrazine in the range of 0.031~0.153 mg/ml(r=0.9998).the average recovery of ligustrazine was 100.95%,rsd=1.75%.

作者单位:广东药学院中药化学与分析教研室,广东广州 510006;河南中医学院,河南郑州 450008 目的建立脑脉通有效部位中总生物碱的含量测定方法。方法采用酸性染料比色法,以溴甲酚绿缓冲液作为生物碱的显色剂,测定样品溶液在417 nm波长处的吸收度。结果对照品川芎嗪质量浓度在0.031~0.153 mg/ml的范围内与吸光度呈良好的线性关系,r=0.9998。平均回收率为100.95%,rsd为1.75%。精密度和稳定性良好。

Was studied by Folin Ciocalteu colorimetry. The results showed that polyphenol content of C. album could be well calculated according to their colorime tric absorption at 765nm by applying Folin Ciocalteu reagent 1.5mL and 10% Na2CO3 6 mL,2 h at 30℃ for colorimetric reaction, and the linear range of standard curve was 7-70mg/L.The method established was very convenient, stable and accurate.Key words: Canarium album Rauesch.;polyphenol;colorimetry

结果表明:橄榄多酚提取液在加入Folin Ciocalteu试剂1.5 mL,10% Na2CO3 6 mL,反应时间2 h,温度30℃的条件下进行显色,并于765 nm处测定其吸光值,多酚质量浓度在7~70mg/L范围内与吸光值有良好的线性关系,根据此线性回归方程进行橄榄多酚的定量测定,该方法简单方便,稳定性好,准确度高。

Methods: water decocting method for extracting method, ether as extraction solvent with high performance liquid chromatography, as the means, to test content of methanol 0-3 % diethylamine (73:27) as mobile phase, 242nm detected wavelength, according to the packing: Hypersil BOS C18 5 muon m size: 6 mm (4): 25 mm, standard curve basic linear, precision, accuracy, good stability and.

以水煎煮法作为提取方法,乙醚作为萃取溶剂,以高效液相色谱作为含量检测的手段,以甲醇-0。 3 %二乙胺(73∶27)作为流动相,检测波长242nm,依利特填料:Hypersil BOS C18 5μm 尺寸:4。 6 mm ×250 mm ,结果:标准曲线基本呈线性,精密度,稳定性和准确度良好。

To establish a MPLC method for the content determination of resveratrol in Polygonum cuspidatum Sieb. Et. Zucc, Analytical column SOURCE 5RPC ST 4.6/150 was used with a mixture of acetonitrile+water (30+70,volume ratio ) as mobile phase ,with a flow rate of 1ml/min and the UV detection wavelength was 306nm, The linear range was 0.6-1.6mg/ml (r=0.9919). The average recovery of prodatin was 103.0%-90.4%(RSD=7.21%). The method is convenient and accurate. Result indicated a reverse phase chromatography media based o n polystyrene/divinyl benzene has excellent resolution for resveratrol in cuspidatum. P rovide theoretical support for process development.

色谱条件采用SOURCE 5RPC ST 4.6/150预装柱,以乙腈/水(体积比30/70)溶液为流动相,流速1ml/min、检测波长为308nm,结果表明,白藜芦醇在0.6~1.6mg/ml的浓度范围内峰面积积分值与浓度的相关系数r=0.9919;所测定的虎杖饮片中白藜芦醇平均含量为0.304%,平均回收率为103.0%~90.4%;实验表明,以聚苯乙烯为基架的反相介质对于虎杖中自藜芦醇具有良好的分辨率,适合其有效成分的检测、分离,对虎杖中白藜芦醇制备的科学研究过渡到工艺开拓有很好的借鉴。

For content determination, the linear range of loganin was 0.084 μg~0.840 μg, r=0.9999, the average recovery was 98.5%(n=6) and RSD was 1.29%.

HPLC测定的马钱苷在0.084~0.840μg范围内呈良好的线性关系,r=0.9999平均回收率为98.50%,RSD为1.29%。

The influence of silt particle content on dynamic strength is a monotonically linear.

粉土中粘粒含量和粉土含量对其动强度的影响规律需从粉土的微观结构角度解释。

The selective linear dimerization of isoprene to formate myrcene in a single phase catalyst solution prepared by mixing two metals, amine, solvent and a small content of isoprene was studied.

摘 要:研究了异戊二烯在两种金属催化作用下均相线性二聚生成月桂烯的影响因素,得到优惠工艺条件:反应温度 13℃,催化剂∶异戊二烯=0 。0 5∶1 0 ,胺∶异戊二烯=0 。0 7∶1.0。

The process of the nonexchangeable potassium release from the soil tested in 912 hours can be well described with linear equation.④ The constant B rate of the nonexchangeable potassium release from the soil tested is controlled by the content of clay and slowly available K.

关于非交换钾的释放动力学,Martin等已用几种微酸性砂质土和砂壤质土进行了初步研究[7~10],国内在此领域研究不多,对中国北方广泛分布的黄土性土壤非交换钾释放动力学研究更少。

The carboxylic content in oxycellulose was analyzed quantitatively by FTIR using KSCN as internal standard. Calibration curve with good linear relationship was obtained.

以KSCN作为内标,用傅里叶变换红外光谱法对氧化纤维素中的羧基含量作了定量分析,得出了线性关系较好的标准曲线。

Stem and Dongmu-70 rye grass (Secale cereale L.) stem. The diameter, length, weight and linear density of stem were measured after sample collection. Shearing force was measured. Meanwhile, the content of moisture were measured, neutral detergent fiber acid detergent fiber lignin, cellulose and hemicellulose were measured. Three Xiaowei sheep were used to determine in situ degradability in 48 h of DM and NDF of alfalfa stem and rye grass stem.

采集2种牧草后,测定茎直径、长度、重量和线性密度,测定剪切力、中性洗涤纤维、酸性洗涤纤维、木质素、纤维素及半纤维素含量,用尼龙袋法测定干物质、中性洗涤纤维48h瘤胃降解率,比较各项指标随茎直径变化的规律,探讨剪切力与各项指标之间的相关性。

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