查询词典 line of flow

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

In agriculture, introduced a total investment of 60,000,000 yuan Zhangjiajie in Hong Kong and Vietnam's pig production and processing projects with a total investment of 12,000,000 yuan in Guangxi oak, Jiang Yun-Rosin Rosin's line item; in the flow of commerce in the area, the introduction of a supermarket Fung Choi, Maney Supermarket, High Tech Computer City furniture and other large-scale supermarkets, 6; in the development of urban construction, the completion of the He Long Sports Center, Division race, Kim Garden, Kangxi Trade City, pedestrian film, the natural gas pipeline network, such as a large number of investment projects Building.

在农业方面，引进了总投资6000万元张家界港越公司乳猪生产加工项目，总投资1200万元广西橡州运江松香公司松香生产线项目；在商贸流通领域，引进了丰彩超市、梅尼超市、宏达家私城等各类大型超市6家；在城市建设开发方面，完成了贺龙体育馆、科赛、金豪花园、康茜商贸城、影视步行街、天然气管网等一大批招商项目的建设。

Methods: Using the techniques of cell culture ex vivo, flow-cytometry, immunocytochemistry, the effects of GPS on the proliferation of human promyelocytic leukemia cell line(HL-60) and human granulocytopoiesis and monocytopoiesis were observed.

结果：GPS在体外能抑制HL-60细胞增殖，而同等剂量的GPS能明显促进正常粒单系造血祖细胞的增殖分化；在本实验条件下未见GPS对HL-60细胞有明显的诱导分化作用。

The optimization models which take into account the representation of FACTS devices, the line flow and the node-voltage constraints and based on simulated annealing is developed. For continuous variables with upper and lower limits, a method of outgrowing adjacent states is proposed.

5针对含FACTS装置的有功优化潮流问题进行了探讨，建立了基于交流潮流算法的含FACTS装置的有功优化潮流数学模型；在此基础上，运用模拟退火算法实现了该问题的求解。

Examination of the equilibrium status of a point in the salt water zone and the description of piezometric heads at two points in both the salt water and fresh water zones reveals that the location of the fresh water-salt water interface in a coastal homogeneous, isotropic unconfined aquifer can be estimated based on piezometric heads at two points in the same vertical line tapping respectively the salt water zone and the fresh water zone (from the water table to the interface) when the groundwater flow system is in a steady state and satisfies the Dupuit assumption.

通过考察咸淡水界面之下咸水带任意点的压力平衡关系和咸水带及淡水带任意点的水头描述得知，当地下水流处于稳定状态且满足Dupuit假设时，可以根据同一垂直线上界面之下咸水带任意点的咸水测压水头和界面之上淡水带任意点的淡水测压水头确定滨海均质各向同性潜水含水层咸淡水界面的位置。

The safe, steady and economic operation as well as the adjustment and control of pneumatically conveying system rely upon the on-line continuous measurement of the mass flow rate of solids phase.

固相质量流量的在线连续测量是气力输送系统安全、高效、稳定、经济运行的前提，是对系统进行调节、控制的依据。

An experiment on forming of cross-hatch on the ablation material surface has been done under the condition of Mach 5 and tur-bulent boundary layer regime.Artifitial disturbances created by randomly setting several pins along a line perpendiculer to the flow direction have shown stochastical character of local cross-hatch and independence of global orderly structure,therefore the pattern appeared fractal behavior.

在外流M＝5湍流边界层状态下，模型为170×300mm￣2蜂蜡平板上进行了烧蚀花纹图案生成的实验研究，通过实验证明了人工安置干扰源并不影响烧蚀材料表面花纹的有序结构，对大面积烧蚀花纹的分析表明，局部花纹结构具有随机性质，因此烧蚀花纹具有分形结构的特性，用分形方法可以大致模拟花纹生成图案。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

Methods With direct-immunofluorescence flow cytometry,expression of Fas/APO-1 in unsynchronized and synchronized cells of PC-3M cell line derived from non-androgen dependent prostatic carcinoma was detected.

采用直接免疫荧光流式细胞术对前列腺癌细胞系PC-3M未同步化的细胞，及经改良的笑气阻断及TdR双阻断方法同步化的不同细胞周期细胞表面Fas/APO-1表达情况进行检测。

The stress analysis and the fatigue life loss calculation of steam drum are carried out in this article. A simplification formula is proponed to calculate the main stress and is used in configuration of DCS. The rain-flow method is used to count the fatigue cycle, which can satisfy the need of on-line monitoring.

本文对其进行了应力分析和疲劳寿命损耗的计算，并提出主应力计算的简化公式用于DCS组态，采用雨流法作为在线计算的疲劳计数方法，满足了寿命损耗在线监测的要求。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。