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Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Because do not have chemical composition completely remain, avirulent, and the limitation that does not have rudimental period, so, apply to green food production, organic agriculture and collect close period still need to continue the crop of gush medicine.

因无化学成分残留、完全无毒，且没有残留期的限制，所以，适用于绿色食品生产、有机农业和采收期仍需继续喷药的作物。

Shows how to play AVIs from resource files, overcoming the major limitation of the Microsoft control.

演示了如何从资源文件中播放AVI文件，克服了Microsoft 控件的一些主要的限制。

The advantage and limitation of using DGGE in uncovering genetic diversity and the potential role of endophtic bacteria in phylogeny and synchronic evolution among the Azolla, cyanobacteria and bacteria were discussed.

文中也讨论了应用DGGE研究遗传多样性的优点、不足以及内生细菌在满江红－蓝藻共生体的系统发育和协同进化中的可能作用。

The air trapping volume had an obvious correlation with FEF50% and MMEF(X^2=10.48, P.01). Conclusions: The CT manifestations of prognosis are demonstrated later than bacteriology. There is a limitation in assessing the activity of pulmonary tuberculosis only by CT signs. CT plays a role in demonstration of secondary small airway changes after bacteriologically cured.

肺结核CT征象转归滞后于细菌学转归，仅凭CT征象对治疗后肺结核活动性判定存在限度；CT对肺结核细菌学治愈后的继发小气道病变的评价有较高价值。

Edit in what defend Jian measure respect, the main opinion that gathers before this includes: It is to suggest not to lengthen the orgnaization that protect Jian to doing poineering work board the superintend and director of the company guides period, card inspect can think through research, because do poineering work board company dimensions is relatively lesser, management stability is relatively inferior, lengthen superintend and director to guide appropriately period be helpful for active and reliable roll out do poineering work board, reason did not grant to adopt; 2 it is to suggest exempt does poineering work board company IPO in those days namely the responsibility of the person that protect Jian of deficit, card inspect can think via research, although do poineering work board the company is managed not stable, but if the company appears on the market in those days with respect to deficit, can produce baddish effect to the market, because this cannot loosen the responsibility of pair of people that protect Jian to find out; 3 it is to suggest to unlock person of the delegate that protect Jian to sign odd number to measure limitation, meeting general is keeping next card watch in be being arranged related Jian system, give further research, hear opinion of orgnaization of Jian of be bailed out, make arrangement jointly.

在保荐办法的修订方面，此前收集到的主要意见包括：一是建议不要延长保荐机构对创业板公司的督导期，证监会经过研究认为，由于创业板公司规模相对较小，经营稳定性相对较低，适当延长督导期有利于积极稳妥推出创业板，故未予采纳；二是建议豁免创业板公司IPO当年即亏损的保荐人责任，证监会经研究认为，尽管创业板公司经营不稳定，但如果公司上市当年就亏损，对市场会产生较坏的影响，因此不能放松对保荐人的责任追究；三是建议放开保荐代表人签单数量限制，下一步证监会将在保荐制度相关安排中予以进一步研究，听取保荐机构意见，共同做出安排。

The wavelet transform suffers from the limitation of poor directional selectivity. To overcome this disadvantage, a novel scheme with joint Contourlet and Bandelet transform is proposed.

针对小波变换方向选择性差的局限，提出了一种基于Contourlet的Bandelet变换。

The bathochromic shift was mainly due to the limitation of rotational movements of the dye. The rigidity of the silica surface restricted the freedom of the molecules. The lower polarity of microenvironment at the silica/water interface than that in aqueous environment should also make a contribution to the phenomenon.

通过对其本体、界面荧光光谱的分析，观察到R6G分子在亲水石英/水界面上出现5 nm红移，红移的原因主要是染料分子旋转运动受到限制，石英表面的刚性限制了分子的自由度，同时，石英/水界面更低的极性也对红移产生影响。

In this paper the course of development from dotted to lined and plane sounding technique is reviewed and the recent application of side scan sonar and laser to the bathymetry as well as its limitation is discussed.

本文回顾了航海测深技术从点测深向线和而状测深的发展过程，探讨了当前旁测声纳和激光在海洋测深方面的应用及其局限性。

Based on the dynamics analysis of the fastener that suffered impact or alternating load, an effective design scheme of the hydraulic power mechanism of the fastener tranverse viberation bedstand with the limitation of the oil power is presented in this paper.

在对紧固件承受横向冲击或交变载荷时进行动力学分析的基础上，对紧固件横向振动试验台在油源功率受限的条件下的液压动力机构的设计问题，提出了有效的解决方案，即用相对较大的动力机构的功率配置来降低系统对油源功率的要求，同时用双阀并联的方法克服了大流量伺服阀频宽较低对系统频宽的影

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。