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Methods:Wistar rats were divided randomly into sham-operation group(n＝6) and ischemia- referfusion group (n＝12).Common carotid artery and external carotid artery were ligated,internal carotid artery and pterygopalatine artery were undivided.Pricking with eye scissors at the distal end of CCA for intralumin suture.By adjusting the suture arc direction to avoid entering the pterygopalatine artery,then withdraw the suture for referfusion 1 h later.

采用健康雄性Wistar大鼠随机分为假手术组(n＝6)和缺血再灌注组(n＝12)，其中缺血再灌注组结扎颈总动脉和颈外动脉，颈内动脉和翼腭动脉不予分离，眼科剪在CCA远端上剪一小口插入线栓，通过调整线栓弧度方向来避免进入翼腭动脉，造成缺血，1 h后外拉栓线形成再灌注。

The cohesive ends of each DNA reanneal and are ligated together . The resulting recombinant DNA molecules are introduced into bacterial host cells .

DNA 片段的粘性末端退火，连在一起，产生的重组 DNA 分子被引入细菌宿主细胞。

During the second stage of subtraction, samples A and B (having different ligated adaptors) are mixed and allowed to reassociate.

在第二阶段的减法，样品A和B的混合和允许reassociate 。

Methods (1) Through microsurgical direct destruction of the arterial intima and internal elastic lamina at the bifurcation of the carotid artery in 30 rats,saccular aneurysms can be induced immediately, and the contralateral carotid arterys were ligated in half of t...

(1)通过显微手术方法直接破坏大鼠颈动脉分叉部位的内膜和内弹力层，建立大鼠实验性囊性动脉瘤模型，并在结扎及未结扎对侧颈总动脉的情况下，观察4-5个月。

Shandong sinder technology limited company,qingdao 266061,shandong,chinaabstract:to provide the eukaryotic expression vector carrying 6x histidine-tag and human growth hormone in expremental animal and cell culture,according to the published nucleotide sequence of the lactofericin b and human growth hormone,synthesis sequence was ligated with pmt-18 vector.

以牛乳素为目的基因，构建含有组氨酸标签、人生长激素信号肽的真核表达载体，为利用牛乳素在活体动物及培养细胞中的表达提供基因材料。

METHODS: The neonatal and adult rats facial nerve was ligated at stylomastoid foramen.

于新生及成年大鼠茎乳孔处结扎面神经，于3， 7， 14， 21， 28， 56， 84 d采用NADPHd组织化学方法观察NADPHd阳性细胞在面神经核中的变化。

Torenia fournieri genomic DNA was digested with DraI、EcoRV、PvuII、SmaI respectively. A special adaptor was ligated to the ends of the digested DNA fragments as a template for adaptor PCR. With the adaptor and TfPLC1 gene-specific primers, bands of 798bp and 813bp upstream of TfPLC1 were obtained successively.

应用衔接头PCR技术，以蓝猪耳全基因组DNA分别经DraI、EcoRV、PvuII、SmaI消化后与衔接头连接产物为模板，用衔接头引物和TfPLC1基因的特异引物经过多轮的巢式PCR，先后克隆到两个大小为798bp、813bp的TfPLC1基因上游序列；经测序、blastn比较分析和拼接得到一个蓝猪耳TfPLC1基因的启动子序列，共1432bp。

The other 98 cases were treated with laser, radiofrequency or ligation for saphenous vein trunk.; cut, electric coagulation, transfixation for vein clusters as additional methods.Perforators were also cutted and ligated as well.

对于伴有交通支功能不全的患者，术中结扎或切断交通支，对于严重深静脉瓣功能不全的患者，同时处理深静脉瓣。

Orientalis in red blood cells from an infected calf was isolated. cDNA was synthesized by reverse transcriptase, amplified by PCR and ligated into λTriplEx2 vector. The recombined vectors were packaged and the unamplified cDNA library was constructed.

用东方巴贝虫感染牛，提纯红细胞内虫体的总RNA，反转录合成cDNA， PCR扩增后将其连接于噬菌体载体（λTriplEx2），通过体外包装，建成东方巴贝虫cDNA文库，并进行扩增。

In this study, the DNA fragments coding for amino acids 133～158 of VP1and 20～34 of VP4protein of type Asial FMDV were chemically synthesized and ligated into a tandem repeat 133～158-20～34-133～158. The sequences of signal peptide of Igκ chain and Kozak were fused to the 5'end of this tandem sequence and synthetic oligodeoxynucleotide containing CpG-ISS was fused to downstream of terminal coden of this tandem sequence. And then this long fragment was cloned into the eukaryotic expression plasmid pHook-2, forming a new secreted expression plasmid, named pAS1-E.

在第二章证明了亚洲Ⅰ型口蹄疫病毒VP1蛋白中133～158位氨基酸残基确是一重要B细胞中和抗原表位的基础上，依据第一章获得的口蹄疫病毒亚洲Ⅰ型VP1 cDNA序列及已报道Asial VP4序列，采用真核偏爱密码子化学合成了VP1中编码133～158位氨基酸及VP4中编码20～34位氨基酸这两个抗原表位基因，将其组成133～158-20～34-133～158串联结构，在其5′端加上鼠Igκ链信号肽序列，在翻译调节区加上增强表达的Kozak序列，同时在133～158-20～34-133～158串联结构3'端终止密码子下游加上CpG免疫刺激序列，将这些片段连接后，克隆到真核表达载体pHook-2上，构建成功了DNA疫苗重组表达载体pAS1-E。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。