查询词典 ligated

Methods We ligated the perineal urethra of the adult female guinea pigs' and then performed punctio vesicae 6 weeks later, followed by a filling cystometry. And then we observed the bladder pressure, capacity changing and the probability of detrusor instability.

成年雌性豚鼠经会阴部尿道结扎，6周后行膀胱穿刺充盈性膀胱测压，观察膀胱压力、容量变化及逼尿肌不稳定的发生情况。

In HMI group and AAP10 group, the circumflex coronaries were ligated. In sham group, left thoracotomy was performed without coronary ligation. The animals were feed three months before the study and an arterially perfused rabbit left ventricular wedge preparation was made.

Sham组开胸但不结扎冠状动脉，HMI组和AAP10组开胸并结扎冠状动脉左室支制备心梗模型，普通饲料喂养三个月后制备兔左心室楔形心肌块的灌注模型。

Coding sequence of pig adiponectin was digested with restriction endonuclease Aval, 267bp fragment of N terminus and 372bp fragment ofC terminus were purified and ligated.

用限制性内切酶Ava Ⅰ处理脂联素编码区，回收N端267bp和C端372bp片段，连接，然后进行PCR扩增，从而得到竞争性模板。

The cloned Buchnera groEL genes of Schizaphis graminum and Rhopalosiphum maize were ligated into pBV221,pET30a and pTrcHisA expression vector for construction of pBVRM,pBVSG, pETRM,pETSGPR,pTrcRM and pTrcSG. They were transformated into E. coli BL21(DE3),DH5a and JM109 for studying their expression.

2将克隆的玉米蚜和麦二叉蚜Buchnera groEL基因连接到原核表达载体pBV221、pET30a和pTrcHisA上，构建了含2个目的基因的6个表达原核表达载体：pBVRM、pBVSG，pETRM、pETSG和pTrcRM、pTrcSG。

An improved method was used to make the inferior caval vein ligated in SD rats of thrombosis model group.

目的 建立一种适合动态研究的大鼠深静脉血栓动物模型。

The morphologic changes of the liver,heart and kidney in relation to the serum choleic acids in obstructive jaundce of rats were investigated.One of the results showed that one to two weeks after the common bile duct was ligated and servered,the damages to the damages to the mitochondria were found in the organs,while the serum choleic acids markedly increased.

对阻塞性黄疸模型鼠的心脏、肝脏和肾脏的形态学改变及其与胆汁酸血症的关系进行了研究，结果表名，胆道梗阻1~2周，心脏、肝脏和肾脏的形态学改变及其与胆汁酸血症的关系进行了研究。

In other three groups, the left coronary artery was ligated to establish myocardial infarction models. Following 15 minutes of coronary artery deligation, the chest was opened. Rats in the cotransplantation group were treated with 250 μL (1×1010/L) BMSCs suspension + 8 mg/L Salvianolic acid B-treated EPC suspension 250 μL (1×108/L) in five points of the myocardium surrounding the deligation region. Rats in the BMSCs group received 500 μL BMSCs suspension. Rats in the model group were injected with an equal volume of cell medium.

结扎冠状动脉15 min后再次开胸，共移植组吸取骨髓间充质干细胞悬液250 μL(浓度1×1010 L-1)+ 8 mg/L丹酚酸B预处理的内皮祖细胞悬液250 μL(浓度1×108 L-1)，于结扎区附近心肌组织分5点注射；骨髓间充质干细胞组同法注射单纯骨髓间充质干细胞悬液500 μL，模型组注射等量细胞培养液。

After the digestion by EcoR I/Sal I or Sal I/Hpa I respectively, the two digestional fragments were ligated with the digestional fragment of pMSCVneo by EcoR I/Hpa I.

构建乙肝缺陷病毒的工作虽然存在难度，但通过制备乙肝缺陷病毒治疗HBV感染的构想是可行的。

The linear plasmid and the designed fragment were directively ligated with T4 DNA ligase,and the plant expression vector of carrot afp was constructed.

将目的片段与线性质粒在T4 DNA连接酶的作用下进行定向连接，构建成胡萝卜afp的植物表达载体pBAF。

Methods The promoter of hTERT and Cox-2 was subcloned from human genomic DNA, then ligated to shuttle vector pd306 which contains the Ela and Elb gene of adenovirus to control the expression of Ela and Elb,respectively.

将hTERT和Cox-2启动子从人类白细胞基因组中亚克隆出来，并将启动子分别插入到腺病毒穿梭载体pd306上的E1A和E1B基因前，使hTERT和Cox-2启动子分别调控腺病毒必须基因E1A和E1B的表达，再将构建后的pd306和腺病毒的骨架质粒BHGE3在Ad293细胞内进行同源重组，并用重组后的病毒感染Hela细胞检测病毒对肿瘤细胞的杀伤力。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了