查询词典 ligated

Results A 357 bp DNA band was seen in the 2% agarose gel after the PCR product was electrophoresed. After the 357 bp DNA band was recovered and ligated into pMD-18T, human transforming growth factor-beta 3 encoding gene acquired through four-cycle PCR was confirmed by sequencing.

结果：4次PCR后，经2％的琼脂糖凝胶电泳可见一357 bp的条带，将电泳产物回收，连接入pMD-18T载体，经测序分析证实，所获得DNA片段为人转化生长因子β3的编码基因。

The diseased lung tissues were serially ligated with the Roeders knot and the hilum of the lung tightly locked with a Rumel's hemostat. The raw surface of the resected lung was sutured.

术中采用Roeder结套扎肺组织或创用Rumel止血器锁紧肺根部、腔镜下缝合肺残端创面等方法处理病肺。

Ultrasound,ERCP,MRCP,liver enzyme profile and reoperation confirmed the folowing: Extra-hepatic bile duct or the right hepatic duct was ligated in 9 cases,common bile duct residual stone in 6,partial gallbladder or gallbladder duct stone in 4,biliary leak in 4, cholangitic hepatitis in 4,hepatitis B or posthepatitis cirrhosis in 5,icterus after blood transfusion in 2,hemobilia in 2, ligation of right branch of hepatic artery in 2, and icterus of unknown causes in 5 cases.

经B超，ERCP，MRCP和肝酶谱及再手术等证实：肝外胆管或右肝管结扎9例，胆总管遗漏结石6例，残留胆囊或胆囊管结石4例，胆瘘4例，毛细胆管肝炎4例，乙肝或肝炎后肝硬化5例，输血后黄疸2例，胆道出血2例，肝右动脉结扎2例，不明原因黄疸5例。再手术18例，死亡1例。结论：详细的病史资料、充分的术前检查、细致的手术操作是预防胆囊切除术后黄疸的必要前提。

Meanwhile, human transferrin minigene was ligated with goat beta lactoglobulin promotor as control. Then, these expression elements were cloned into lentivirus vector.

在此基础上将上述表达单元装入慢病毒载体构建了转铁蛋白慢病毒表达载体。

Moschus berezovskii IL-2 was cloned from Con-A stimulated peripheral blood mononuclear cells, and ligated with pIVID18-T vector, then transformed to Escherichia coli JM109. The positive recombinant was sequenced.

用林麝外周血单核细胞(peripheral blood mononuclear cells， PBMC)提取总RNA，RT-PCR扩增IL-2(interleukin-2， IL-2)，连接pMD18-T载体后转化大肠杆菌，筛选阳性克隆并测序。

The structure gene pelA from Thermotoga maritima MSB8 encoding pectate lyase was amplified and ligated into pHsh, resulting pHsh-pelA. Through structural optimization on pHsh-pelA, the ultimate plasmid, pHsh-pelC, which possessed the most appropriate structure and free energy of mRNA, was obtained.

将来源于极端嗜热菌属海栖热袍杆菌Thermotoga maritima MSB8的编码碱性果胶裂解酶的结构基因pelA与新型热激质粒pHsh连接，得到重组质粒pHsh-pelA，运用mRNA二级结构预测软件对pHsh-pelA的翻译起始区的二级结构进行优化，得到了具有最佳mRNA二级结构及自由能的质粒pHsh-pelC。

In order to clone the VIP gene in the gastrointestinal tract from beijing duck, one pair of specific primers to VIP gene was designed and synthesized according to the chick sequence (X80906). Encoding VIP cDNA fragments were amplified by RT-PCR from the total RNA in the Proventriculus, the Duodenum and the Jejunum of Beijing duck. Their PCR products were ligated into pGEM-T easy vector, which was transformed into E. coil JM109. Positive bacteria clones were screened and identified by PCR method and digested with the double restriction enzyme EcoRⅠ. The sequence of VIP gene fragment was also determined and analyzed.

为从北京鸭胃肠道中扩增血管活性肠肽基因，根据鸡VIP基因（GenBank登录号X80906），设计了一对简并引物，从北京鸭腺胃、十二指肠和空肠提取总RNA，通过反转录－聚合酶链反应扩增，将从腺胃、十二指肠和空肠中扩增出的产物克隆到pGEM-Teasy载体上，导入大肠杆菌JM109，阳性克隆经双酶切鉴定后测序，将测序结果与鸡和鹅（GenBank登录号为DQ023161）的VIP基因进行同源性比较。

To construct a retroviral vector carrying immortalizing gene hTERT, screen gene puro, reporter gene EGFP and recombination site sequence LoxP for reversible cell immortalization. Linearized pBABE-puro was cut by restriction enzyme Nhe Ⅰ and ligated with loxP double strands to construct a vector pBABE-puro-lox.

目的 探讨以hTERT作为永生化基因、嘌呤霉素乙酰转移酶基因puro作为筛选基因、加强型绿色荧光蛋白EGFP作为报道基因、loxp作为重组位点构建可回复性永生化逆转录病毒载体，为建立可回复性永生化细胞株莫定基础。

Objective The arteria carotis externa was ligated, and arteria carotis interna was kept opening The left carotid artery was exposed and placed over an ultrasonic flow probe. The brain flow volume was recorded with an ultrasonic volume meter. The pursiness time of mice was observed to evaluate the effect of Erigeron breviscapus pellets on cerebral ischemia.

目的 应用二道血流速度流量仪超声探头测定大鼠颈总动脉以反映云南植物药业有限公司提供的灯盏花颗粒对脑血流量的影响；记录小鼠断头后张口喘气时间，观察灯盏花颗粒的抗脑缺血作用。

Objective The arteria carotis externa was ligated,and arteria carotis interna was kept opening The left carotid artery was exposed and placed over an ultrasonic flow probe.The brain flow volume was recorded with an ultrasonic volume meter.The pursiness time of mice was observed to evaluate the effect of Erigeron breviscapus pellets on cerebral ischemia.Methods Born's method was used to investigate effects of Erigeron breviscapus pellets on rabbit platelet aggregation induced by adenosine diphosphate,arachidonic acid,and platelet-acti-vating factor,in vitro or in vivo.Antithrombotic effects of Erigeron breviscapus pellets was monitored accord-ing to Peter's method.

目的 应用二道血流速度流量仪超声探头测定鼠颈总动脉以反映云南植物药业有限公司提供的灯盏花颗粒对脑血流量的影响；记录小鼠断头后张口喘气时间，观察灯盏花颗粒的抗脑缺血作用方法采用Born方法测定灯盏花颗粒在体内外对AA、ADP及PAF诱导的家兔血小板聚集功能的影响；运用电刺激大鼠颈动脉血栓形成方法，评价灯盏花颗粒对血栓形成的阻抑作用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。