查询词典 ligated

In this study, the left circumflex arteries of rabbits were ligated to obtain the animal models of AMI.

目的： 1。采用开胸、结扎兔冠状动脉方法，建立AMI动物模型。

Methods The anterior descending and circumflex branches of the left coronary artery were ligated and infarction in the wall of left ventricle was created in six dogs.

结扎6只犬心冠状动脉左前降支及左旋支的分支，拟在左心室壁造成梗死区。

Methods: Under sterilization, swines were opened chest and ligated left circumflex coronary artery.

无菌条件下开胸结扎猪冠状动脉左回旋支，建立AMI动物模型。

Protoscoleces of cyst were recovered from the brain of sheep naturally infected with coenurus of Taenia multiceps and total RNA was extracted.T.multiceps thioredoxin peroxidase cDNA fragment was amplified by RT-PCR from the total RNA,ligated into pMD18-T vector and sequenced.

从自然感染的病羊脑内采集多头蚴原头节，提取总RNA，采用RT-PCR技术扩增多头带绦虫硫氧还蛋白过氧化物酶基因片段，PCR产物连接到pMD18-T载体，转化至大肠杆菌DH5α后测序，发现克隆到的TmTPx基因片段开放阅读框为453bp，编码150个氨基酸。

Materials and Methods 20 cases of local ,healthy, crossbred pigs , including 9 males and 11 females ,their common bile ducts were ligated.

材料与方法健康杂种家猪20只，雄性9只，雌性11只，行胆总管结扎，建立梗阻性黄疸动物模型。

Two 15nt complementary ssDNA primers were ligated into double stranded satRNA.Complete sequence of satRNA was obtained by RT-PCR using these 15nt ssDNA as amplification primers.Phylogenetic analysis showed that the identity of this 383nt satellite and some documented satRNAs was 72.6 to 99.5% at the nucleotide level.

以常规引物对感病植物的总RNA进行RT-PCR扩增，获得CMV RNA3全长克隆，经测序显示该RNA3序列属于CMV亚组Ⅱ；通过在卫星dsRNA两端分别加上15nt的单链DNA接头，以接头互补序列为引物进行扩增获得一个383nt的卫星RNA的全长序列。

Methods Thirty domestic dogs were randomly divided into 5 groups (6 in each group ): model control group, positive control group, and TGP groups with doses of 2.0, 4.0, and 8.0 mg·kg-1, respectively. The descending branch of left coronary artery was ligated to construct the model of myocardial infarction in the anesthetic thoraco-opened dogs. The effect of TGP on epicardium electrocardiogram , myocardial infarction size and serum myocardial enzymes were observed.

取杂种犬30只，随机分为5组（每组6只），分别为模型对照组、阳性对照药物组及TGP 2.0、4.0和 8.0 mg·kg-1剂量组；采用结扎麻醉开胸犬左冠状动脉前降支制备急性心肌梗塞模型，观察TGP对心外膜电图、心肌梗塞面积和血清心肌酶的影响。

Methods The LPL cDNA was isolated from the human epiploon adipose tissue by means of RT-PCR. The LPL cDNA was ligated into the pcDNA3.1Zeo. The recombinant pcDNA3.1Zeo-LPL cDNA was identified by endonucleases, PCR and DNA sequencing. COS-1 cells were transfected with the recombinant LPL gene plasmid using Lipofectamine 20001M The LPL mass in cells and the culture medium were determined by a Markit-M LPL Kit. Spectrophotometry was used to measure the LPL activity.

采用RT-PCR法从人大网膜脂肪组织获取LPL cDNA基因，以pcDNA3.1Zeo质粒作为载体，运用基因克隆技术构建野生型人LPL cDNA重组质粒，限制性内切酶酶切、PCR以及双向测序鉴定重组质粒；运用Lipofectamine 2000将重组pcDNA3.1 Zeo-LPL cDNA质粒导入COS-1细胞，RT-PCR法检测LPL mRNA，ELISA法和比色法分别检测细胞裂解液及细胞培养基中LPL浓度及活性。

Itis a great deal of assistment for treatment and prognosis of patients, if we candiagnose hepatic fibrosis in forepart. The aim of this experiment evaluate the dignosisvalue of ultrasound elastic imaging on early hepatic fibrosis.we will supply thefundermental data for reseach henceforth. 2、Materials and Method The number of Wister rats is 30 and weight is 180-220g. 30 Wister rats had beenligated on the right hepatic ducts (include middle lobe、right lobe and caudate lobe) tocause partial cholestatic hepafibrosis factitiously and divided in A, B and C groups. Aand B groups had been ligated on the right hepatic ducts both. The different with Aand B is that the time of A group after ligation on the hepatic ducts is 2 weeks and thetime of B group is 1 week.

将实验动物分为A、B、C三组，A组为结扎大鼠右侧胆管(包括中叶、右叶、尾状叶)2周后进行检查。B组为结扎大鼠右侧胆管1周后进行检查，C组行假手术为对照组。A组大鼠麻醉后，腹部备皮，皮肤常规消毒，腹部横行切开，暴露肝脏、胆总管，游离右侧胆管，结扎，然后关腹；C组，仅开腹然后关腹，即假手术组。A、C两组同批实施手术，A、C两组术后1周，B组实施手术，步骤、方法、部位同A组。

Itis a great deal of assistment for treatment and prognosis of patients, if we candiagnose hepatic fibrosis in forepart. The aim of this experiment evaluate the dignosisvalue of ultrasound elastic imaging on early hepatic fibrosis.we will supply thefundermental data for reseach henceforth. 2、Materials and MethodThe number of Wister rats is 30 and weight is 180-220g. 30 Wister rats had beenligated on the right hepatic ducts (include middle lobe、right lobe and caudate lobe) tocause partial cholestatic hepafibrosis factitiously and divided in A, B and C groups. Aand B groups had been ligated on the right hepatic ducts both. The different with Aand B is that the time of A group after ligation on the hepatic ducts is 2 weeks and thetime of B group is 1 week.

将实验动物分为A、B、C三组，A组为结扎大鼠右侧胆管(包括中叶、右叶、尾状叶)2周后进行检查。B组为结扎大鼠右侧胆管1周后进行检查，C组行假手术为对照组。A组大鼠麻醉后，腹部备皮，皮肤常规消毒，腹部横行切开，暴露肝脏、胆总管，游离右侧胆管，结扎，然后关腹；C组，仅开腹然后关腹，即假手术组。A、C两组同批实施手术，A、C两组术后1周，B组实施手术，步骤、方法、部位同A组。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了