查询词典 ligated

The external carotid artery was ligated, mobilized, cannulated, and injectate administered.

颈外动脉也结扎、游离、插入导管并进行注射。

MethodsThe external carotid artery of rat was ligated to observe the effect of PAMD on cerebral blood flow、cerebral vascular resistance、blood pressure and heart rate.ResultsPAMD could increase CBF and decrease CVR,but had no effect of cardiac function.

本课题组近年研究表明，PAMD具有较好的抗脑血缺作用，可降低脑组织含水量、抑制丙二醛的产生及增强超氧化物歧化酶的活性。

The left anterior de scending branches were ligated in anesthetic dogs to establish acute myocardial infarction models. The activities of AST, CK and LDH in serum were detected and the changes of ultrastructure of myocardium were observed.

采用麻醉开胸结扎犬的冠状动脉前降支制备急性心肌梗塞模型，取静脉血检测血清中天门冬氨酸转氨酶、磷酸肌酸激酶和乳酸脱氢酶活性；应用透射电子显微镜观察心肌细胞超微结构的改变。

Newly-synthesized DNA strands from adjacent replicons are ligated together.

来自邻近的复制子的新合成的DNA链又连结在一起。

The BDDcFⅧ gene was ligated behind PUB and 20H1 promotors to create lentiviral vectors pTK161 and pTK162. Meantime lentiviral vectors pTK161' and pTK162' were produced by cloning a green fluorescent protein into pTK151 and pTK152, which was driven by PUB and 2OH1 promotors respectively. Vector supernatant were prepared by using transfer calcium phosphate mediated-cotransfection of 293T cells. The virus vector,△NRF packaging-plasmid, and VSV-G envelope-plasmid was assayed by titers and cFⅧ activity in cell culture supernatant after infection into 293T cells.

用构建携带cFⅧ基因的慢病毒载体pTK161和pTK162（含20HI启动子），同时构建含绿色荧光蛋白的慢病毒载体pTK161'和pTK162'（含2OH1启动子）的方法，分别与包装质粒△NRF、包膜蛋白质粒VSV-G共转染293T包装细胞，将包装好的病毒颗粒再感染293T细胞，检测病毒滴度和培养细胞上清中cFⅧ活性。

The genomic DNA of Streptomyces diastaticus SM33 was extracted and partially digested by Hind III. The 40~60 kb high molecular weight DNA was extracted and ligated to pIndigoBAC536. The ligation mixture was transformed into EPI300 competent cells. A total of 5184 colonies were obtained by white-blue screening.

提取淀粉酶链霉菌SM33基因组DNA，用Hind III部分酶解后回收40~60 kb大小的高分子量DNA，与质粒载体pIndigoBAC536连接，电击转化EPI300感受态细胞，经蓝白斑筛选共挑取了5184个白色克隆。

The genomic DNA of Streptomyces diastaticus SM33 was extracted and partially digested by Hind Ⅲ. The 40~60 kb high molecular weight DNA was extracted and ligated to pIndigoBAC536.The ligation mixture was transformed into EPI300 competent cells. A total of 5 184 colonies were obtained by white-blue screening.

提取淀粉酶链霉菌SM33基因组DNA，用HindIII部分酶解后回收40kb~60kb大小的高分子量DNA，与质粒载体pIndigoBAC536连接，电击转化EPI300感受态细胞，经蓝白斑筛选共挑取了5184个白色克隆。

Subtractive hybridization was performed by annealing an excess of driver cDNA with each sample of adaptor ligated tester cDNA. The cDNAs were heat denatured and incubated in 68℃ for 8 hours.

杂交产物经过LA Taq DNA聚合酶补平末端后，以试剂盒提供的PCR Primer 1作为第一次PCR的引物，以Nested Primer 1和Nested Primer 2R作为第二次PCR的引物，通过抑制性PCR富集大量在Tester中高表达的差异表达基因的cDNA。

Objective To observe the morphological changes of pancreatic tissue of thoracic duct ligated rats in fine and ultrastructural levels, and to determine whether lymph block animal model can affect pancreatic islet amyloid polypeptidedeposit in rat pancreas.

目的 观察胰淋巴淤滞动物模型胰组织结构变化和胰淀肽沉积情况，探讨淋巴淤滞对胰组织细微和超微结构的影响。

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

The production moved off-Broadway to New York City, where she won the Joseph E. Calloway award for her performance, and was profiled in American Theater Magazine as one of ten young theater artists to watch.

生产提出过百老汇的新的纽约市，在那里她赢得了约瑟夫calloway奖，她的表现，并于异型在美国的战区杂志之一， 10战区的青年艺术家观赏。

It's a risk tradeoff, and I believe that adding test support code usually wins.

这是一个风险的权衡，我相信添加测试代码通常会占上风。