查询词典 ligated

METHODS: The left fifth lumbar nerve of rats was tightly ligated with silk sutures under pentobarbital anesthesia.

在使用戊巴比妥麻醉的情况下，将老鼠的左侧第五腰神经用缝线结扎起来。

An additional 10 rats served as shamoperation group, the LAD was braided, but not ligated.

另随机选取10只大鼠作为假手术组，只在其左前降支下穿线不结扎。

Then the PCR products were purified and ligated with PGEM-T-easy Vector.

经PCR鉴定正确后，再进行斑点杂交试验、序列分析和同源性比较。

The deep artery branch should been ligated to harvest enough pedical when it is necessary.

逆行转移时，皮瓣尽可能的设计在近段，蒂部尽可能保留2～3个分支，以保障皮瓣的血供。

Bam HI partial digestion fragments from Pseudomonas mallei chromosome were ligated into the Bam HI site of the cloning vector pBR 322 and tranferred into E.

用&鸟枪法&分子克隆技术将鼻疽杆菌基因重组到载体质粒pBR 322的BamHⅠ酶切位点上，再转化到大肠杆菌RRⅠ中。

University of Tokyo , Tokyo 11328657 , JapanAbstract : The genome DNA was extracted from the blood of six horse breeds including SH , B H ,XN , WZ , TB , AI. Four pair of primers were designed based on the sequence of human , mouse and ferret DRD4 gene cDNA to amplify the corresponding horse gene. The PCR products were ligated into a linear plasmid pMD17 , and sequenced respectively.

从6 个品种马的血液中提取基因组DNA ，根据GenBank中已发表的人、鼠、雪貂的DRD4 cDNA 基因保守区序列，设计3 对特异性引物，另1 对由文献所得，对6个品种马的DRD4 基因分4 段进行PCR 扩增，经电泳检测，分别呈4条特异条带，将其分别克隆入pMD192T 载体中，进行序列测定并拼接序列，得到包括所有内含子的DRD4 基因序列。

I, Shannon Blood Sausage. Stand-pig small intestine washed first with warm water to soak rice for half an hour, picked up the rice, adding blood, mix with the rice and put into a large stone or blood beater, then add green onion, salt, has been Net diesel refining pig, star anise powder mix, wash the pig has been poured into the small intestine, ligated at both ends into the pot, cook the back, pay attention to the fire can not be too fast and can not boil water, to keep turning in order to avoid open rupture Blood Sausage, and come to naught, cooked to salvage, you can cut consumption, increase can also be cooked pan fried dishes, the use of soy sauce, vinegar, monosodium glutamate pair of points is expected, it is delicious, Miao can not be made.

一、香血肠。将猪小肠洗净备用，先用温水将大米浸泡半个小时，捞起白米，加入猪血，与米搅匀，放入大石磨或打浆机磨成血米浆，再加入葱花、盐、已炼的猪网油渣、八角粉等拌匀，灌入已洗净的猪小肠中，结扎两端，放入锅内，慢火煮熟，注意火不能太猛，水不能烧开，要经常翻动，以免血肠破裂开，而前功尽弃，煮熟后捞起，即可切块食用，也可用菜锅加熟油煎后，用酱油、醋、味精配成的点料，更是味香可口，妙不可言。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Then,the left external carotid artery of 40 adult New Zealand White rabbits were ligated,which reduced the pulsatile shear stress of left common carotid significantly (for example, mean=21.16 ± 7.17 vs 3.13±2.28 dynes/cm2, p=2.176E-21)and did not change the pulsatile shear stress of right common carotid significantly.

结果左颈外动脉结扎后即刻兔的左颈总动脉形成脉动低切应力以τ_(mean为例，从21.16±7.17dynes/cm~2降至3.13±2.28dynes/cm~2，p=2.176E-21，并一直持续至结扎12周后；而右颈总动脉脉动切应力始终无显著变化。

MethodsThe external carotid artery of rat was ligated to observe the effect of PAMD on cerebral blood flow、cerebral vascular resistance、blood pressure and heart rate.

方法采用结扎颈外动脉的方法，观察PAMD对大鼠脑血流量、脑血管阻力、血压、心率等的影响。结果PAMD可增加脑血流量，降低脑血管阻力，对心脏功能无明显影响。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了