查询词典 ligated

The genome DNA was extracted from the red blood cells or blood of the infected sheep and goats which include as isolates of ovine Babesia from these different geographic areas. The gene specific primers were devised according to the pubulished 18srRNA sequence at GenBank. The target DNA segment was amplified by polymerase chain reaction, the PCR product was ligated into PGEM-Teasy vector.

用分离于四个地区的羊的巴贝斯虫感染实验动物，自感染羊的红细胞或全血中提取基因组DNA，根据GenBank中已发表的巴贝斯虫18S rRNA序列设计两对羊巴贝斯虫特异性引物，通过PCR扩增目的基因片段，将扩增产物连接到PGEM-Teasy载体中，经酶切鉴定，PCR分析，进行序列测定，结果显示羊巴贝斯虫四个分离株的18S rRNA基因大小为1800bp左右。

Methods:Both common carotid arteries were ligated bilaterally in 70 Wistar rats.

目的：探讨星形细胞在慢性脑灌注不足脑损害中的作用。

Methods Cadmium-specific promoter PcadA was obtained by PCR from pPcadA and was ligated to E.coli-B.subtilis shuttle vector pNGFP with gfp being reporter gene ,after which the recombinant plasmid was transformed into 1A751 strain to form the bio-engineered bacteria.

采用聚合酶链式反应从质粒pPcadA上扩增得到重金属镉离子特异性启动子PcadA，以绿色荧光蛋白基因为报告基因，以大肠杆菌-枯草杆菌穿梭载体pNGFP构建了绿色荧光蛋白镉离子诱导表达载体。

The effects of electroacupuncture on the pressor response and reversible myocardial dysfunction induced by application of bradykinin on the gallbladder were studied in cats anesthetized with α-chloralose. The cardiovascular responses evoked by application of BK included a pressor response, an increase of LVP and its dP/dtmax, tachycardia and a decrease of local wall motion of the left ventricle with a supplying branch of the left anterior descending coronary artery ligated beforehand.

在α-氯醛糖麻醉的猫上，结扎左心室冠状动脉前降支一小分支，对动物胆囊浆膜面予以缓激肽，可反射性地诱发血压、左心室内压升高、±dP/dtmax增大、心率加快，同时用超声单晶片声纳测微系统记录局部心壁运动，发现BK可以反射性地引起LAD小分支远端所支配的心肌运动功能减弱。

METHODS Neonatal HIBD models were made according Rice method, new-born rabbits wereanesthetized by aether inspiration, and their right common carotid arteries were ligated.Experimental animals were put into the hypoxic champer (8%FIO2) for two and anhalf hours. Control groups included five ligated animal which didnt undergo hypoxicmanagement.

实验方法按照Rice法制备新生兔缺氧缺血性脑损伤模型，即用乙醚吸入麻醉动物，5％盐酸利多卡因局部浸润麻醉，颈部正中切口，剥离右侧颈总动脉和迷走神经，用2号缝线将剥离出的右侧颈总动脉作永久结扎。

Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

Method］twenty six wistar rats were divided into two groups, with 13 in each group, peroneal nerve was transacted on the right side of all the animals, in one group, the proximal end of peroneal nerve was turned back and ligated, the distal end was sutured to the side of tibial nerve with perineurial window, in another group,both ends of the transacted peroneal nerve were turned back and ligated as contrast, no operation was done on the left side.

方法］wistar大白鼠26只，随机分实验组和对照组，每组13只，实验组右侧腓总神经切断后，近端反转结扎，远端与胫神经外膜开窗处行端侧吻合，对照组右侧腓总神经切断后，两端均反转结扎，两组动物左侧作正常对照，不作处理。术后3个月，行双侧腓总神经、胫前肌组织学检查，胫前肌湿重测定，运动终板检查。

Methods Fifteen rabbits were divided into three groups: group A (posterior limbs was completely cut off and posterior tibia artery was ligated with posterior tibia vein), group B (posterior limb was uncompletely cut off and posterior tibia artery was ligated with posterior tibia vein), group C posterior limb was completely cut off and traditional operation type was applied to ligate the posterior tibia artery with posterior tibia artery (control group.

用15只兔分3组：A组为完全离断小腿胫后动脉缝接胫后静脉组，B组为部分离断小腿胫后动脉缝接胫后静脉组，C组为完全离断小腿应用传统的胫后动脉缝接胫后动脉对照组，3组均结扎胫前动脉，保留胫前静脉等未动脉化的静脉及缝接其它离断软组织。

Methods The left anterior descending coronary artery was ligated and gas embolism was performed through vein tube inserted distal to the ligated site of LAD in 6 dogs,while 8 dogs underwent ligation of LAD.cTnI,CK,CK-MB,LDH and electrocardiographic parameters were evaluated before,and 30min,60min,90min,120min,and 180min after occlusion.

最后用红四氮唑和伊凡氏蓝染色确定心肌梗死区和缺血危险区并称其湿重，计算二者比值。结果结扎前降支血管后30、60、90、120、180min时间点，实验组cTnI、CK、NST和∑ST较对照组明显高，差异有统计学意义(P.05)，在部分时间点LDH、CK-MB亦升高(P.05)。实验组心肌梗死与缺血危险区比值(0.58±0.16)%较对照组(0.29±0.31)%明显高，差异有统计学意义(P.05)。对照组2例(25%)发生致死性室颤。

Methods: Under peridural anesthesia, the right rectus incision was adopted. First, the vessels in lesser curvative of stomach was amputated and ligated. Second, splenic artery was ligated to deflate spleen .Then ,splenic artery and renal vein were amputated and side-to-side anastanosised with each other. Result: Of the 36 cases,the portal free pressure decreased by different degree in 23 cases, unchanged in 10,and increased in 3 postoperatively. The follow-up ranged from 1 to 5 years.

在硬膜外麻醉下经右腹肌切口进腹、采用先作胃小弯的血管彻底离断，再结扎脾动脉、使脾脏缩小后，而后分离出脾静脉和肾静脉进行端侧吻合，结果：36例中23例病人吻合后测得门脉自由压均较分流前有不同程度的降低，10例门脉自由压未变化。3例高于分流前压力。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了