查询词典 ligate

On the other hand,the Inverse PCR combined with a tail-to-tail primer design requires T4 DNA ligase enzyme to ligate PCR products,which makes the positive rate relatively low.We aim to find out an easy and effective method to perform gene deletion, insertion and substitution mutation.

近几年，有文献报道使用基于Quikchange原理的方法来制作DNA片段的插入、替代以及删除突变，但由于QCM-PCR的低产量，难以产生阳性克隆。

Methods 98 patients underwent total thyroidectomy or subtotal thyroidectomy. During operation, the parathyroid glands were exposed and preserved, and the trunk of inferior thyroid arteries were avoided to ligate. After operation, the parathyroid function was observed.

对98例行甲状腺全切、次全切除术在术中显露保护甲状旁腺，避免结扎甲状腺下动脉主干，观察术后甲状旁腺功能情况。

Methods Groups 12 rabbits were divided randomly two groups: Control group (n=6): ligate the left anterior coronary descending branch for 40 mm and I Oml/kg balanced salt solution was reperfused for 40 mm after removing the ligation.

第四军医大学硕士学位论文一、实验方法实验分组 l 健康家兔12只，按随机分组原则分为两组：0）对照组的只）：结扎冠状动脉左室支，心肌缺血40min后解除结扎，再灌注40min。

The method causes bandicoot full head to be short of hematic model through 4 arterial ligate, applied NO electrode is short of blood to reach in full head to area of bandicoot sea horse again change of chroma of the NO below perfusion condition undertakes real time detects.

梗概： 目的探索大鼠海马区在全脑缺血及再灌注状态下NO浓度变化的动力学。

Coll. Methods Insert the synthetic SARS-CoV N protein gene into vector pUC-18,ligate to plasmid pET28 and transform to E.

方法将已合成的SARS-CoV N蛋白基因片段，克隆至pUG18载体，与pET28质粒连接，转化大肠杆菌，进行SARS-CoV核衣壳融合蛋白表达。

METHODS: Fortyfive aged healthy crossbred dogs (15±7) kg were divided into 3 groups: tetramethlpyrazine group, verapamil group and control group. The operation was performed to ligate the near part of the LAD through left minimal thoracic incision to block 75% of the blood flow respectively. The expression changes of endothelium derived factors NO, ET1, sPselectin and CTnT before ligation and at different time points after ligation were detected and Pselectin gene of cardiac muscle was determined by Western blotting.

将45只犬分为川芎嗪，维拉帕米和对照组3组，结扎LAD阻断血流75%，分别测定3组结扎前及结扎后不同时段的分泌型P选择素、内皮素1（ET1）、一氧化氮及心肌肌钙蛋白T的含量变化，Western blotting法检测心肌组织Pselecntin基因表达情况。

METHODS: Fortyfive aged healthy crossbred dogs (15±7) kg were divided into 3 groups: tetramethlpyrazine group, verapamil group and control group. operation was performed to ligate the near part of the LAD through left minimal thoracic incision to block 75% of the blood flow respectively. The expression changes of endothelium derived factors NO, ET1, sPselectin and CTnT before ligation and at different time points after ligation were detected and Pselectin gene of cardiac muscle was determined by Western blotting.

将45只犬分为川芎嗪，维拉帕米和对照组3组，结扎LAD阻断血流75%，分别测定3组结扎前及结扎后不同时段的分泌型P选择素、内皮素1（ET1）、一氧化氮及心肌肌钙蛋白T的含量变化，Western blotting法检测心肌组织Pselecntin基因表达情况。

METHODS: Forty five aged healthy cross bred dogs (15±7) kg were divided into 3 groups: tetramethlpyrazine group, verapamil group and control group. The operation was performed to ligate the near part of the LAD through left minimal thoracic incision to block 75% of the blood flow respectively. The expression changes of endothelium derived factors NO, ET 1, sP selectin and CTnT before ligation and at different time points after ligation were detected and P selectin gene of cardiac muscle was determined by Western blotting.

将45只犬分为川芎嗪，维拉帕米和对照组3组，结扎LAD阻断血流75%，分别测定3组结扎前及结扎后不同时段的分泌型P 选择素、内皮素 1（ET 1）、一氧化氮及心肌肌钙蛋白T的含量变化，Western blotting法检测心肌组织P selecntin基因表达情况。

Isolate, ligate, and divide the anterolateral malleolar and lateral tarsal arteries and carefully expose the neurovascular bundle and retract it medially.

沿切口方向切开骨膜、关节囊及滑膜，通过骨膜下及关节囊下分离，显露整个踝关节的前方。

Isolate, ligate, and diide the anterolateral malleolar and lateral tarsal arteries and carefully expose the neuroascular bundle and retract it medially.

沿切口方向切开骨膜、关节囊及滑膜，通过骨膜下及关节囊下分离，显露整个踝关节的前方。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了