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An expressed sequence tag analysis was performed to identify major genes involved in silkworm gonad development. 16737 ESTs were generated by sequencing cDNA clones from the two libraries (8407 from testis library and 8330 from ovary library), and assembled into 2107 contigs and 3532 singlets.

利用表达序列标签方法分析参与家蚕性腺发育的基因,通过测序获得16737条ESTs(精巢8407条,卵巢8330条),拼接这些ESTs共得到2107个重叠群和3532个单拷贝。

The taking shape of the edgy intellec intelletuals intellectual is a inevitable outcome under non-planned economy condition,Through inital explored on the aspect of reproofing of Marginal intellectuals,the paper pointsout the ineffective factors of the library assistance and pats forward the necessity and feasibility of library assistance.

论文关键词边缘知识人/图书馆/援助,论文来源图书馆L,论文单位长沙,点击次数 0,论文页数 16~17,57页2004年2004月论文网 http://www.lw23.com/paper_32947531/边缘知识人是非计划经济条件下的必然产物,本文通过对边缘知识人在再提高方面的初步探讨,指出了图书馆对其援助不力的因素,提出了图书馆对其帮助的必要性和可行性。

Records of the library students, The library number is not renewals.

记录学生的借书情况,借书的次数,是不是续借。

Staborton university library has a limited number of shared and individual l ocked carols for faculty and graduate students who are working on research re quiring library resources.

斯戴伯尔敦大学图书馆有一些数量有限的可上锁的合用学习室或独用学习室,供需要利用图书馆资料进行科研的教师和研究生之用。

New laccase gene and its full-length cDNA were clone from genomic library and equalized cDNA library of Ganoderma lucidum. Similarity between the sequence and other laccase gene were analyszed with NCBI blast, the results show that the new gene belong to multicopper oxidases gene family and have 60-70% Similarity with other laccase gene.

从灵芝的基因组文库和cDNA均一化文库中克隆了灵芝的漆酶基因和全长cDNA,并通过NCBI Conserved Domain Search氨基酸序列的相似性分析及与其它真菌漆酶之间的相似性分析,证明得到的蛋白是一种新的multicopper oxidases,其蛋白质序列与其它已克隆漆酶基因的同源性在6 0~7 0%之间,并对论文克隆的漆酶基因的启动子和终止子进行了分析。

In what District of Columbia Public Library Chief Librarian Ginnie Cooper calls an effort to make DCPL more secure and welcoming, the library has proposed banning sleeping in its facilities and a limit of two bags per person.

在哥伦比亚特区的公共图书馆馆长吉利库珀要求努力使图书馆更安全和更欢迎,图书馆已提议禁止睡在其设施内并限制每人只能带两个袋子。

Mr. Thoreau explained to the President that the railroad had destroyed the old scale of distances,--that the library was useless, yes, and President and College useless, on the terms of his rules,--that the one benefit he owed to the College was its library,--that, at this moment, not only his want of books was imperative, but he wanted a large number of books, and assured him that he, Thoreau, and not the librarian, was the proper custodian of these.

梭罗向馆长解释:现在火车已摧毁或扩大了借阅距离,----图书馆也就变得无用,是的,馆长和大学也就变得无用,在他自己看来,这所大学唯一对他的用处就是它的图书馆,----眼下,他不但是急用这些书,他还急用更大数量的书,而且梭罗向馆长保证,他,梭罗,而不是管理员是这些书的合法看管人。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板,挑取其中的白斑经活化培养后,运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板),即5,000 余个重组子克隆的高质量质粒DNA;提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction,聚合酶链式反应)扩增出重组子插入片段,标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5,000 余条5′端EST 序列的测定工作,初步筛选出4,747 条质量较好的EST序列,经筛选得到的EST中90%以上具有大于500bp 的可读序列,每一EST序列中不可读碱基数小于5%。

This paper points out that the library should establish the modern information knowledge service and resource guaranteeing system under the guidance of the scientific concept of development,and expounds the thought of people-based concept and reader first in the modern library.

阐述了现代图书馆&以人为本,读者第一&的思想,指出了图书馆应在科学发展观指导下,建立现代信息知识服务和资源保障体系。

Realizing the uncertainty legal statute of the "fair use" issue concerning digital library, this article moves to rethink the current copyright law and proposes to introduce the "implied license theory" and a digital library safe harbor into the copyright law.

在认识到Google数字图书馆在法律上是否构成合理使用的不确定性和模糊性之后,试图从理论上对著作权法进行反思,并提出在著作权法中引入&默示许可制度&和&数字图书馆'安全港'条款&的构想。

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推荐网络例句

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。