查询词典 library binding

We have obtained 16 cDNA fragments binding to the elicitor response cis-element from rice cDNA expression library using yeast one-hybrid system.

利用酵母单杂交体系，我们从水稻的cDNA库中分离到16个阳性克隆。

This sample, and accompanying article, will illustrate how to implement and call Automation interfaces that support VTBL binding by providing a type library and by using the marshaller provided by Automation.

安装本更新程序可添加一些新功能，通过这些新功能，您可以使用新版本的 GDIplus 来控制不同文件格式的呈现。

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中，得到的重组质粒命名为pGBKT7-Gα，β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性，将其转化到酵母菌pJ69-4A中，再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子，用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库，ELISA鉴定阳性克隆，DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列；免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

Results After screening, 59 subtracted library clones were isolated which were specific for strain VIB72, and the DNA sequences of these clones were determined. Seventeen fragments showed high homology to the genes of known functions in other bacteria. This includes soluble lytic murein transglycosylase, mobilization protein, transposase (IS66), resistance-related protein (metallo-beta-lactamase and acetyltransferase family), toxin protein (DT-201 and alveicin A immunity protein), ATP-dependent endonuclease of OLD family like protein, SocE and GTP-binding protein HflX (high frequency of lysogenization).

通过对差减文库筛选，分离到59个对菌株VIB72的克隆，并对这些克隆的DNA序列进行了测定。17个基因片断与其它细菌的已知功能的基因有较高的同源性，其中包括可溶性溶胞壁质转糖基酶、转移蛋白MobA和MobC、转座子IS66、抑制相关蛋白（金属β-内酰胺酶和乙酰转移酶家族）、毒素蛋白（DT-201和alveicin A免疫蛋白）、与OLD 家族相似的ATP依赖性核酸内切酶以及SocE 和GTP结合蛋白HflX。

The open reading frame of cellular nucleic acid-binding protein was cloned from Grass Carp cDNA library.

从草鱼肝肾cDNA文库中克隆到细胞核酸结合蛋白基因CNBP的完整开放阅读框序列。

ABSTRACT In order to screen chicken heart type fatty acid-binding protein gene genomic DNA library was constructed in λ phage.

本研究通过构建北京油鸡基因组DNA噬菌体文库，筛选含鸡心脏型脂肪酸结合蛋白基因的重组子，克隆出4株含鸡H-FABP基因片段的重组噬菌体。

ABSTRACT In order to screen chicken heart type fatty acid-binding protein gene genomic DNA library was constructed in λ phage. Four positive clones containing chicken H-FABP gene were obtained.

本研究通过构建北京油鸡基因组DNA噬菌体文库，筛选含鸡心脏型脂肪酸结合蛋白基因的重组子，克隆出4株含鸡H-FABP基因片段的重组噬菌体。

Binding is the library every year or every other year must be done and when bookbiding journals the most appropriate?

期刊装订是图书馆每年或隔年必须要做的工作，而什么时候装订期刊最为合适呢？

In our work to select high affinity antibodies from reshaped phage antibody library against human bladder carcinoma, we found a panel of antibodies with much different antigen binding activity in library screening had similar relative affinity constants.

从抗人膀胱癌改形噬菌体抗体库中筛选出一组相对亲和力常数和细胞结合活性相近的单链抗体，而它们的结合活性均明显高于起始的鼠源单链抗体。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。