查询词典 leucine

Methods Metabolic functions were studied by antipyrine autoradiography for the measurement of cerebral blood flow,by 3H-leucine autoradiography for the measurement of cerebral protein synthesis and by regional ATP bioluminescent imaging for the measurement of ATP content.

方法用放射性自显影方法测定L1 KO小鼠和野生型对照组小鼠大脑局部血流量和蛋白质合成率，用生物荧光影像技术测定两组ATP含量，并将两组数据进行比较。

Methods Metabolic functions were studied by [14C] antipyrine autoradiography for the measurement of cerebral blood flow, by 3H-leucine autoradiography for the measurement of cerebral protein synthesis and by regional ATP bioluminescent imaging for the measurement of ATP content.

用放射性自显影方法测定L1 KO小鼠和野生型对照组小鼠大脑局部血流量和蛋白质合成率，用生物荧光影像技术测定两组ATP含量，并将两组数据进行比较。

Objective: To set up a method for determination of arginine and leucine in human fibrin sealant kit.

目的：建立HPLC法测定人纤维蛋白粘合剂中盐酸精氨酸和亮氨酸的含量。

Auricularia auricula polysaccharide to mice with intraperitoneal injection of 100mg/kg continuous 4d, pairs of 3H leucine incorporation serum protein had a weaker role in promoting.

黑木耳多糖给小鼠腹腔注射100mg／kg连续4d，对3H亮氨酸掺入小鼠血清蛋白有较弱的促进作用。

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只，每组再分为高代谢脓毒症组和对照组两部分，建模方法同第一部分；2）三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸；3）灌注前抽取动脉血作为背景值，灌注达150，160，170和180min分别抽取动脉血2ml用于质谱分析；4）实验开始后前30min中每5min收集呼出气一次，随后每30min收集呼出气一次，用于气相质谱分析；5）血样经过低温离心取上清液，过阳离子及阴离子交换树脂，再经衍生化处理；6）处理过的血样进入气相色谱－质谱仪，测量其13C标记的亮氨酸、葡萄糖和乳酸丰度；7）实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度（E13CO2）；8）利用文献提供的公式算出CO2总生成率（V13CO2）以及机体各物质的通量、氧化速率以及物质代谢百分比等；9）数据分析处理采用t检验。

Owing to the abroad application of Candida rugosa lipase in the resolution of racemic aminoacides, the prepared lipase was used in the resolution of phenylglycine and tert-leucine. Through the optimization of the reaction conditions, such as reaction substrate, pH, temperature, ion strength and additives, enantiomeric excesses for phenylglycine and tert-leucine reached 96% and 90.1%, respectively, enantiomeric ratio came up to 28. 9 and 15. 4, respectively.

鉴于该酶广泛应用于手性化合物的拆分研究，本论文中选用了两种手性氨基酸作为拆分对象，通过对反应体系中一系列条件的优化，包括底物、pH、温度、离子强度以及添加物的使用，苯甘氨酸和叔亮氨酸的对映体过量值分别达到96%和90.1%，对映催选择率分别达到28.9和15.4。

N L-Leucine Producer,Strain No .145,was obtained from mutants resistant to α-amino-β-hydroxyl valeric acid and rifampin by mutagenizing Brevibacterium Tianjinese T6-13 with ul-traviolet rays,It produced L-Leucine up to 32mg/mL on shaker and 21.8mg/mL of L-Leucine was produced in 16 liter fermentor in 60 hours.

以天津短杆菌T6～13为出发菌株，采用紫外线进行诱变处理，筛选α－氨基－β－羟基戊酸和利福平抗性突变株，获得一株L－亮氨酸产生菌No.145，L－亮氨酸摇瓶产量可以达32.1mg／mL。在16L发酵罐中培养60h，L－亮氨酸产量可以达21.8mg／mL葡萄糖转化率可以达20％。

The linear concentration ranges for γ-aminobutyric acid, valine and leucine were 5.0×10^(-3)-0.12 g/L, 5.0×10^(-3)-0.08 g/L and 5.0×10^(-3)-0.12 g/L, respectively, and the detection limits (S/N=3) were 2.5×10^(-3), 2.5×10^(-3) and 2.6×10^(-3) g/L, respectively. The relative standard deviations of the peak currents of γ-aminobutyric acid, valine and leucine were 2.5%, 4.9% and 3.9%(n=7), respectively.

氨基丁酸、缬氨酸和亮氨酸的线性范围分别为5.0×10^(-3)~0.12、5.0×10^(-3)~0.08和5.0×10^(-3)~0.12 g/L，检出限(S/N=3)分别为2.5×10^(-3)、2.5×10^(-3)、2.6×10^(-3)g/L；7次平行进样峰高的相对标准偏差分别为2.5%、4.9%、3.9%。

The basic leucine zipper protein that containing leucine zipper domain and basic domain are one unique kind of transcription factors/repressors in plants.

bZIP蛋白是植物中特有的一类转录因子，包含亮氨酸拉链结构域和碱性结构域两部分。

A pair of optical isomerism amphiphlic molecules L-C10HLN-(4-decaoxygen-2-hydroxybenzene methylene-L-leucine and D-C10HLN-(4-decaoxygen-2-hydroxybenzene methylene-D-leucine were synthesized.

製备了一对旋光异构的两亲分子L-C10HL（N-（4-癸氧基-2-羥基苯亚甲基）-L-亮氨酸）和D-C10HL（N-（4-癸氧基-2-羥基苯亚甲基）-D-亮氨酸）。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。