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Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

You never got rid of downlording songs every week or morth even there was lest than 1G in your mp3 only .

那时的你，就算Mp3只有不到1G，你也乐此不彼天天塞着耳塞，每隔一个星期或一个月去下载更新。

Speculative reason could only exhibit this concept problematically as not impossible to thought, without assuring it any objective reality, and merely lest the supposed impossibility of what it must at least allow to be thinkable should endanger its very being and plunge it into an abyss of scepticism.

投机性的原因只能出现此概念problematically作为并非不可能想到，没有任何保证它的客观现实，否则，只是在它必须至少是不可想像的，应允许危害应该是不可能的非常幸福和投身到它怀疑的深渊。

I trembled lest he should send me to call her; but I was spared the pain of being the first proclaimant of her flight.

我直发抖，唯恐他差我去叫她。可是我倒免掉作第一个宣告她的私逃的人这场痛苦了。

I trembled lest he should send me to call her; but I was spared the pain of being the first proclaimant of her flight.

但是我相信一个能够在事先就计划出怎样利用她的暴躁脾气的人，即使在爆发的时候，也可以行使她的意志，努力控制她自己；而且我也不愿如她所说去&吓唬&她的丈夫，只是为了满足她的自私而增加他的烦恼。

It showed the widest inhibition zone against Pythium ultimum (7.5mm),while it was lest effective to unknown species. The size of inhibition zones on those eleven tested isolates were all less than 3.0 mm.

用不同的培养液培养LT6所得的代谢物，在相同浓度下，对同一腐霉菌菌丝体生长的抑制效果不同，培养液1/5 TSB和NB明显强于KMB。

When having good thing of course, natural meeting introduces him, lest reoccupy idea goes talking with him, ripe can believe you.

当 然有好东西的时候，自然会介绍给他，免得再专心思去跟他谈，熟了就会相信你。

For instance,"You affirm first receive money, lest forgot to moment, I am couldn't get ' reputably '."

比如，&你先确认收货，免得到时候忘记了，我得不到'好评'。&

Make up 40% sugar juice now, using sugar volume and raw material to compare is 1: 0.7, 0.2% citric acid are added in sugar juice, because watermelon skin organization is harder, can appear candy law to heat with boil make, cast the watermelon skin that cent has cut into to boil make, it is OK to begin big a crucial moment heats, make its fast evaporate moisture, boil raw material to become transparent all the time, when sugar juice concentrates ceaselessly and showing syrup, should be to use small fire to undertake heating, lest produce anxious saccharify to make product darkly Brown, determine sugar juice chroma is achieved 70% when should stop to heat.

现配制40%糖液，用糖量与原料比是1：0.7，在糖液中加入0.2%柠檬酸，因西瓜皮组织比较坚硬，可用一次透糖法加热煮制，把分切好的西瓜皮投进一起煮制，开始可以大火候加热，让其快速蒸发水分，一直煮到原料变得透明，糖液不断浓缩而呈糖浆时，应是用小火进行加热，以免产生焦糖化使产品呈黑褐色，测定糖液浓度达到70%时就应停止加热。

I have never seen a man taller than him.I think he is the tal 107 lest one in the world.

2我再也没有见过任何一个人比他更高，我认为他是世界第一高人。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。