查询词典 leave it at that

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

People familiar with Barclays' thinking said it was looking at two options for a sale of the pound;4bn of assets ndash; either a replica of the Protium deal that would see Barclays traders leave with the portfolio, or a divestment to a third-party buyer.

了解该行想法的人士表示，对于这笔40亿英镑的资产，该行正考虑两种出售方式：或者复制Protium交易方式，在剥离资产的同时，相关交易员也随之离去；或者出售给第三方。

All not didn't will falter an own confidence before taking place in the affair result at any time, this all affairs in the world canned is divided into two kinds, oneself can control of with beyond control of, work well to do foot with all one's heart oneself that, leave of smile an arrangement of seeing the destiny, occasionally it would also unfair, but most time destinies praise those persons who make great effort more to have preparation more of prize more.

任何时候在事情结果没发生前都不要去动摇自己的信心，这个世界所有的事情可以分为两种，自己可以控制的和无法控制的，尽心尽力做好做足自己那份，剩下的笑看命运的安排，偶尔它也会不公平，但多数时候命运更褒奖那些更努力更有准备的人。

You're finding things that you didn't know I look at you with such distain The walls start breathing My minds unweaving Maybe it's best you leave me alone.

您找到的东西，你不知道我看你同distain的墙壁开始呼吸我的头脑拆散也许这是最好的，您让我安静。

It convulses him so that he foams at the mouth, and shatters him, and will hardly leave him.

他被鬼抓住就忽然喊叫；鬼又叫他抽疯，口中流沫，并且重重的伤害他，难以离开他。

It is suggested that you leave this on, otherwise your game may have reduced performance as the sound is decompressed real-time at runtime.

我们建议你设置为开，在游戏运行时解压声音将降低你游戏的性能。

He is a passionate believer in free trade (which endears him to this newspaper, at least), and he was a supporter of the "surge" in Iraq when all Democrats, most independents and a fair few Republicans thought the best thing to do was for America to leave. Some of these beliefs, alas, will surely hurt him, but it is much to his credit that when advisers urged him to moderate them he angrily refused.

麦凯恩在许多其他的问题上也都冒着背离主流观点的风险：例如他是自由贸易的狂热信徒，但这样的姿态至少使他受到媒体的追捧；又比如当所有的民主党人、大部分的独立派以及相当多的共和党人都认为美国应该从伊拉克撤军的时候，他却以&伊战风头正劲&说法的坚决支持者形象出现。

The ever more popular Eurostar between London and Paris, Spain's expanding network of AVE high-speed trains, the ICE network in Germany – just as we are beginning to understand that criss-crossing the world by air may leave very little of the world for our children to criss-cross at all, it looks as though an older, slower, greener and altogether more satisfying way of travel has smartened up its offerings.

伦敦与巴黎之间的欧洲之星越来越受欢迎，西班牙的AVE高速铁路网正在不断扩大，而德国也开通了自己的城际列车特快(Inter City Express，简称ICE)。飞机在空中的穿梭往来，也许会让今后的孩子们彻底失去旅行的空间。当我们开始懂得这一点的时候，似乎就能够看到：一种老派一点、缓慢一点、环保一点、并且总体说来更令人满意的旅行方式，就足以提供种种格外诱人的东西。

Sol–gel/diaminobenzoic acid hybrid materials were also generated to be used as the MALDI matrices for the analysis of oligonucleotides. It is because that DABAs have good molar absorptivity at wavelength of 337 nm. Furthermore, two amino functional groups attached to the aromatic ring in DABAs can also suppress the cation adductions of oligonucleotides and leave the protons to the oligonucleotides during MALDI-MS analysis. This matrix system can be used as an alternative for the analysis of oligonucleotides.

本论文也利用双胺基苯甲酸与溶胶凝胶混成产物当做MALDI基质来分析DNA分子，利用双胺基苯甲酸本身具有吸收雷射的特性，且其含胺之官能基可和钠、钾离子竞争DNA上磷酸根的负离子结合部位，因此可和DNA有较强的亲合力，而在脱附游离的过程中并可转移质子给磷酸根，可有效降低盐类的干扰，此一基质系统可取代传统MALDI基质混合胺盐的效果，适合用於DNA样品的分析。

Kayak, with its AJAX interface, makes it easy to quickly display only flights that leave and arrive at certain times, connect through certain airports, have short layovers etc. Yapta's search, on the other hand, doesn't even allow to search for flights to neighboring airports and has no filtering capabilities once the search in completed.

皮艇，其Ajax的界面，让您轻松快速显示，只有航班离开和抵达在某些时候，某些连接，通过机场，有短期layovers等yapta的搜索，另一方面，甚至不容许搜索航班到邻近的机场，并没有过滤功能，一旦在完成搜索。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了