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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.
本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。
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3At first discharge port, lay time shall be commenced at 13:00 if Notice of Readiness is tendered before noon and at 8:00 next day if NOR is given in the afternoon. Time lost in waiting for berth hall be counted as lay time. Time used for shifting the vessel from place of stoppage, lay-by berthto discharge berth, and lighterage/lightening shall be counted as lay time. At second discharge port/lay time shall be commenced to count immediately upon vessel arrival.
在第一个卸货港,如果准备就绪通知在中午前发出,正式卸货时间为13:00,如果准备就绪通知在头天下午发出,正式卸货时间为次日的8:00,在码头上等待时间视为卸货时间,其他如紧急码头停泊等视为卸货时间,在第二个卸货港,船一旦到达开始计算卸货时间。
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A little after Noon I found the Sea very calm, and the Tyde ebb'd so far out, that I could come within a Quarter of a Mile of the Ship; and here I found a fresh renewing of my Grief, for I saw evidently, that if we had kept on board, we had been all safe, that is to say, we had all got safe on Shore, and I had not been so miserable as to be left entirely destitute of all Comfort and Company, as I now was; this forc'd Tears from my Eyes again, but as there was little Relief in that, I resolv'd, if possible, to get to the Ship, so I pull'd off my Clothes, for the Weather was hot to Extremity, and took the Water, but when I came to the Ship, my Difficulty was still greater to know how to get on board, for as she lay a ground, and high out of the Water, there was nothing within my Reach to lay hold of; I swam round her twice, and the second Time I spy'd a small Piece of a Rope, which I wonder'd I did not see at first, hang down by the Fore-Chains so low, as that with great Difficulty I got hold of it, and by the help of that Rope, got up into the Forecastle of the Ship; here I found that the Ship was bulg'd, and had a great deal of Water in her Hold, but that she lay so on the Side of a Bank of hard Sand, or rather Earth, that her Stern lay lifted up upon the Bank, and her Head low almost to the Water; by this Means all her Quarter was free, and all that was in that Part was dry; for you may be sure my first Work was to search and to see what was spoil'd and what was free; and first I found that all the Ship's Provisions were dry and untouch'd by the Water, and being very well dispos'd to eat, I went to the Bread-room and fill'd my Pockets with Bisket, and eat it as I went about other things, for I had no time to lose; I also found some Rum in the great Cabbin, of which I took a large Dram, and which I had indeed need enough of to spirit me for what was before me: Now I wanted nothing but a Boat to furnish my self with many things which I forsaw would be very necessary to me.
午后不久,海面风平浪静,潮水也已远远退去。我只要走下海岸,泅上几十米,即可到达大船。这时,我心里不禁又难过起来。因为我想到,倘若昨天我们全船的人不下小艇,仍然留在大船上,大家必定会平安无事。这时就可安抵陆地;我也不会像现在这样,孤苦伶仃孑然一身了。而现在,我既无乐趣,又无伴侣。想到这里,我忍不住流下泪来。可是,现在悲伤于事无济,我即决定只要可能就先上船去。当时,天气炎热,我便脱掉衣服,跳下水去。可是,当我泅到船边时,却没法上去,因为船已搁浅,故离水面很高;我两臂所及,没有任何可以抓住的东西。我绕船游了两圈,忽然发现一根很短的绳子。我惊异自己先前竟没有看见这根绳子。那绳子从船头上挂下来,绳头接近水面;我毫不费力地抓住绳子往上攀登,进入了船上的前舱。上去后发现船已漏水,舱底进满了水。因为船搁浅在一片坚硬的沙滩上,船尾上翘,船头几乎都浸在水里,所以船的后半截没有进水。可以想像,我急于要查看一下哪些东西已损坏,哪些东西还完好无损。首先,我发现船上的粮食都还干燥无恙。这时,我当然先要吃些东西,就走到面包房去,把饼干装满了自己的衣袋,同时边吃边干其他活儿,因为我必须抓紧时间才行。我又在大舱里找到了一些甘蔗酒,就喝了一大杯。此时此刻,我极需喝点酒提提神。我这时只想有一只小船,把我认为将来需要的东西,统统运到岸上去。
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Through studying the landscape planning and design of the lakeside traveling vacation area,educed the following conclusion: 1st, Ecological consciousness should permeate the development and construction of landscape;basic frame of landscape planning and design in lakefront traveling vacation area: Setting upecological consciousness→establishing the principles of landscape planning and design→theconcept lay: Position analysis, planning orientation→the planning lay: Landscape type analysis, landscape pattern analysis, ecology condign analysis→the design lay: Protection ecologydesign, compensating ecology design, compatible ecology design→establish ecological landscape 2nd, combining the protection first principle with landscape planning and design In planning lay, applying the protection first principle and land utilization "10% principle", plansthe artificial landscape and the management landscape.
通过对滨湖旅游度假区景观规划设计的研究,得出如下的结论: 1、生态意识应贯穿景观开发建设的始终:滨湖旅游度假区景观规划设计的基本框架:生态意识的树立→滨湖景观规划设计原则的确立→概念层面:区位的分析、规划定位→规划层面:景观类型分析、景观格局分析、生态适宜性分析→设计层面:保护性生态设计、补偿性生态设计、适应性生态设计→生态景观的建立。
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But the many people think that the rare egg compares familiar nourishment good, directly use to lay egg to feed, or beat again to lay egg to mix into after the thing cooks, in fact this kind of way of doing very mistake, living of the egg will cancel out each other with the vitamin h occurrence in the Ao body of function, last long to feed on to lay egg, will produce huge breakage to the furriery that hides Ao.
可是很多人认为生的鸡蛋比熟的营养好,就直接用生蛋喂,或者在事物煮熟后再打个生蛋混进去,其实这种做法极其错误,生的鸡蛋会和獒体内的维生素h发生互相抵消的作用,长久喂食生蛋,会对藏獒的毛皮产生巨大的破坏。
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Meditation Alone/ firefly译 The Moon Is Mine The moon is mine And the sundries I home mail It's thin, cool and dark Like me under an unlucky star Who stay out straying, with a sadly sorrowful look When facing it You should feel esteem--in my opinion Also you could Weep when looking at it, and Be put in a tight spot, or Behold a man in it coming out Lay Open the Loneliness Lay open the loneliness, perhaps There be no loneliness Under the alone lamp, in the empty room The whole world's dyed orange with rays Everything represents nothing As someone been far away The deserted heart still misses Only lonely is still lonely Wine for one, when wine round three times for one Think of the drip drops, the fallen leaves, and Some time, some seasons, some persons, some Sundries when warm Like I lay open the loneliness Then nothing to keep lonely The Tree All the leaves in this tree fall Its loneliness falls into a face It's limpid and bright Where the sun shines There are blares of colored glaze Exclaim occasionally, or profoundly Stride one's line of sight, such height and weald Straying there --Why there is no wild Divide the Rain Drips Divide the dense rain drip Then you may see decaying scene-- A man in the sun walking with vigorous strides In the shallow puddle Immerges half his nose, spraying Continuous ripples It's simply a groundless guess at daytime Or rather a dream, yet that's it And that I see Sun's weak, and see The sun and water turns to be blank in the air Nov.
16th, 2007 原诗:《独想》《那些月光是我的》那些月光是我的是我寄给家里的物什它薄薄的,凉凉的,暗暗的就像我的薄命流浪在外,神情悲悯你们面对它应该感到高贵——这是我的想法你们也可以看着它流泪,看着它一筹莫展,或者看着一个人从里面走出来《把孤独摊开》把孤独摊开,或许就没有孤独了在孤灯下,空屋里万物都染着桔色的光万事都不代表什么就像远去了一个人心中空荡荡的,却总有惦念但孤独还是孤独的一个人的酒,一个人酒过三巡想想窗檐的滴水,想想落叶,想想一些时间,一些季节,一些人物,一些那时感到有温度的物什就如同把孤独摊开了没什么可继续孤独的《这棵树》这棵树落光了叶子它的孤独就成了一张脸它清澈与明亮太阳光照的地方,全然是时空中一簇琉璃的光泽偶尔让人惊叹,偶尔骨感地跨入人的视线,那样的旷野和高度停留在那里——为什么没有荒芜《把雨点分开》把稠密的雨点分开就可以看到颓废的景观——一个刚在太阳下健步如飞的男人在浅水坑中浸入了一半的鼻子,喷着连续不断的涟漪这只是我白日的一个臆念有点梦,但就是这样我才看到了阳光的软弱,看到了阳光和雨水原来就是一场空中之茫
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She is not in the washroom and then stopping, because it is finally his own point of view that he cares about and not hers, and with this firmly in mind, and a light, confident step, such as you might find in the works of Bulwer-Lytton, he enters the space she occupies so prettily and, taking her by the hand, proceeds to tear off the stiff white hospital gown (but no, we cannot have that kind of pornographic merde in this majestic and high-minded sentence, which will probably end up in the Library of Congress)(that was just something that took place inside his consciousness, as he looked at her, and since we know that consciousness is always consciousness of something, she is not entirely without responsibility in the matter) so, then, taking her by the hand, he falls into the stupendous white puree of her abyss, no, I mean rather that he asks her how long it has been since her last visit, and she says a fortnight, and he shudders, and tells her that with a condition like hers (she is an immensely popular soldier, and her troops win all their battles by pretending to be forests, the enemy discovering, at the last moment, that those trees they have eaten their lunch under have eyes and swords)(which reminds me of the performance, in 1845, of Robert-Houdin, called The Fantastic Orange Tree, wherein Robert-Houdin borrowed a lady's handkerchief, rubbed it between his hands and passed it into the center of an egg, after which he passed the egg into the center of a lemon, after which he passed the lemon into the center of an orange, then pressed the orange between his hands, making it smaller and smaller, until only a powder remained, whereupon he asked for a small potted orange tree and sprinkled the powder thereupon, upon which the tree burst into blossom, the blossoms turning into oranges, the oranges turning into butterflies, and the butterflies turning into beautiful young ladies, who then married members of the audience), a condition so damaging to real-time social intercourse of any kind, the best thing she can do is give up, and lay down her arms, and he will lie down in them, and together they will permit themselves a bit of the old slap and tickle, she wearing only her Mr.
然后阻止,因为到最后那是他自己的关心的观点而不是她的,在他的脑子里根深蒂固,他自信地走了一步,这就像你也许会在布尔沃?里顿的作品中看到的一样,他进入兴高采烈地进入她占有的空间,抓住她的手,开始撕开他白色僵直的病号衣(但是我们没有在他冠冕堂皇的充满智慧的语句中发现那种带有色情的废话,那也许会在国会图书馆中绝迹)(在他的意识中有一些东西正在被替代,当他看她的时候,就如我们知道的意识始终是意识,她在这个事情中不是完全没有责任的),所以,然后抓起她的手,他掉进了她深渊的巨大白色水中,不,我想他问她自从她上回来已经多久了,她说有两个星期了,他战栗着告诉她她现在的情况(她是一个非常受欢迎的女兵,她的部队通过埋伏在森林里赢得了所有的战斗,在最后的时刻敌人发现了,那些他们吃掉他们午餐的树有了眼睛和剑)(这使我想起了一八四五年罗伯特?乌丹的演出,叫做《奇幻橘子树》,在其中罗伯特?
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She is not in the washroom and then stopping, because it is finally his own point of view that he cares about and not hers, and with this firmly in mind, and a light, confident step, such as you might find in the works of Bulwer-Lytton, he enters the space she occupies so prettily and, taking her by the hand, proceeds to tear off the stiff white hospital gown (but no, we cannot h39e that kind of pornographic merde in this majestic and high-minded sentence, which will probably end up in the Library of Congress)(that was just something that took place inside his consciousness, as he looked at her, and since we know that consciousness is always consciousness of something, she is not entirely without responsibility in the matter) so, then, taking her by the hand, he falls into the stupendous white puree of her abyss, no, I mean rather that he asks her how long it has been since her last visit, and she says a fortnight, and he shudders, and tells her that with a condition like hers (she is an immensely popular soldier, and her troops win all their battles by pretending to be forests, the enemy discovering, at the last moment, that those trees they h39e eaten their lunch under h39e eyes and swords)(which reminds me of the performance, in 1845, of Robert-Houdin, called The Fantastic Orange Tree, wherein Robert-Houdin borrowed a lady's handkerchief, rubbed it between his hands and passed it into the center of an egg, after which he passed the egg into the center of a lemon, after which he passed the lemon into the center of an orange, then pressed the orange between his hands, making it 56aller and 56aller, until only a powder remained, whereupon he asked for a 56all potted orange tree and sprinkled the powder thereupon, upon which the tree burst into blossom, the blossoms turning into oranges, the oranges turning into butterflies, and the butterflies turning into beautiful young ladies, who then married members of the audience), a condition so damaging to real-time social intercourse of any kind, the best thing she can do is give up, and lay down her arms, and he will lie down in them, and together they will permit themselves a bit of the old slap and tickle, she wearing only her Mr.
然后阻止,因为到最后那是他自己的关心的观点而不是她的,在他的脑子里根深蒂固,他自信地走了一步,这就像你也许会在布尔沃?里顿的作品中看到的一样,他进入兴高采烈地进入她占有的空间,抓住她的手,开始撕开他白色僵直的病号衣(但是我们没有在他冠冕堂皇的充满智慧的语句中发现那种带有色情的废话,那也许会在国会图书馆中绝迹)(在他的意识中有一些东西正在被替代,当他看她的时候,就如我们知道的意识始终是意识,她在这个事情中不是完全没有责任的),所以,然后抓起她的手,他掉进了她深渊的巨大白色水中,不,我想他问她自从她上回来已经多久了,她说有两个星期了,他战栗着告诉她她现在的情况(她是一个非常受欢迎的女兵,她的部队通过埋伏在森林里赢得了所有的战斗,在最后的时刻敌人发现了,那些他们吃掉他们午餐的树有了眼睛和剑)(这使我想起了一八四五年罗伯特?乌丹的演出,叫做《奇幻橘子树》,在其中罗伯特?
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"The academy understands each enterprise that garrison very much, to group of their technical actual strength, growing foreground, management, include all sorts of defect and inadequacy, look as clear as dayly ", feng Guanping says, because chicken is in oneself henroost, who can lay who cannot lay, it is the thing of be clear at a glance, be about to give a few nutrition more to the chicken that can lay, and this nutrition is " money, capital, venture capital investment ".
"研究院对进驻的每一家企业都非常了解,对他们的技术实力、成长前景、管理团队,包括各种缺点和不足,都看得一清二楚",冯冠平说,由于鸡在自家鸡窝里,谁能下蛋谁不能下蛋,是一目了然的事情,对能下蛋的鸡就要多给一些营养,而这个营养就是"钱、资金、风险投资"。
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Twenty-six patients had subpulmonary VSD(diameter 0.8±0.8cm), 18 VSD lay immediately below pulmonary valve, 8 had muscle distant from pulmonary valve, 20 lay completely below pulmonary valve, and 6 lay below pulmonary valve and aortic valve.
3肺动脉瓣下型室间隔缺损26例(直径0.8±0.8cm),其中近邻肺动脉瓣18例,与肺动脉瓣有肌性相隔8例;单纯位于肺动脉瓣下20例,主动脉瓣和肺动脉瓣下6例。
- 相关中文对照歌词
- Lonely Goatherd
- Lay It On Me
- Lay It Down
- Lay It Down
- Lay Me Down
- Under The Radar
- Lay You Down
- Lay You Down
- Lay Down
- Lay It Down Part II - A Tribute To The Legends
- 推荐网络例句
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Putt your way through 36 fun-filled holes of minigolf on 3D designed courses with elevated greens, bunkers, bridges and water hazards, among other crazy obstacles.
您的推杆方式,通过36个有趣的填孔迷你的三维设计的课程,以提升绿党,掩体,桥梁和水的危害,除其他疯狂的障碍。
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Some participles can be used either as attributes or as predicatives.
有些分词既可当定语用,也可当表语用。
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Over time, the jaw crusher has been a significant improvement, it is a highly efficient, energy-efficient equipment often broken.
随着时间的推移,颚式破碎机得到很大的改进,已经是一种高效,节能的常用破碎设备。