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The investigation of cells of bronchial lavages with lung cancer showed hypermethylation of at least one gene in squamous cell carcinoma, adenocarcinoma, small cell lung cancer and adenosquamous carcinoma is 17/23(73.9%),13/20(65.0%),4/5 and 2/3, respectively.

51例肺癌患者中,支气管灌洗液和上清研究过甲基化结果如下:对灌洗液细胞进行三种基因过甲基化研究诊断鳞癌、腺癌、小细胞癌和腺鳞癌的阳性比例分别为17/23(73.9%),13/20(65.0%),4/5,2/3。

There is not a significant difference in these groups by one-way analysis of variance. 2 Among 51 cases with lung cancer, 7 specimens showed malignant cells in bronchial lavages, including 4 cases in central lung cancer and 3 cases in peripheral lung cancer. Concordant results were observed in 20 cases. 6 specimens showed malignant cells on cytological analysis, and NMSP was positive in at least one gene tested. 14 samples did not show malignant cells on cytological analysis, and the NMSP results were correspondingly negative. The results of cytology and NMSP were discordant in 31 samples. 30 samples were cytologically negative, but NMSP positive in one or more genes. In addition, there was one case that the cytology was positive for malignant cells, but the NMSP was negative in all of the three gene tested.

51例肺癌患者支气管灌洗液细胞学检查发现肺癌细胞7例,其中27例中心型肺癌患者4例支气管灌洗液发现肺癌细胞,24例周围型肺癌患者3例支气管灌洗液发现肺癌细胞。20例支气管灌洗液细胞学检查和过甲基化检测得到一致结论。6例支气管灌洗液发现肿瘤细胞,过甲基化分析至少一个基因阳性。14例灌洗液未发现肿瘤细胞,三个基因过甲基化分析均为阴性。31例支气管灌洗液细胞学检查和过甲基化检测得到不一致结论,30例细胞学检查为阴性,但过甲基化分析一个或一个以上基因为阳性。1例细胞学检查阳性但过甲基化分析阴性。

The lysozyme activity of pulmonary alveolar macrophages was determined by lysoplate assay method. PAM were obtained from the lungs of 26 normal rats by saline bronchoalveolar lavages.

本文检测了26例大鼠肺泡巨噬细胞溶菌酶活性,酶活性稳定,并与细胞浓度呈直线正相关;大蒜对增强肺泡巨噬细胞溶菌酶活性有显著作用。

Difference of cell-free DNA concentrations was analyzed by one-way analysis of variance using SPSS 10.0 software. There is a significant difference if p value was less than 0.05. The results showed that: 1 DNA concentrations in cell-free lavages supernatants in central lung cancer, peripheral lung cancer and pulmonary benign lesions are (0.83±0.34)μg/ml,(0.66±0.31)μg/ml and (0.59±0.37)μg/ml, respectively.

研究结果如下: 1中心型肺癌支气管灌洗液上清游离DNA量为(0.83±0.34)μg/ml,周围型肺癌为(0.66±0.31)μg/ml,肺部良性病变为(0.59±0.37)μg/ml,经单因素方差分析发现三者支气管灌洗液游离DNA量没有显著差异。

Construct PLXRN-IL-1Ra and PLXRN-IL-10 vector, lapine synoviocytes were first transduced in culture by retroviral infection. The genetically modified synovial cells were then transplanted by intra-articular injection into the knee joints, assay of joint lavages confirmed that the gene expression was not lost after 14 days of transfer. Knees receiving the hIL-1Ra had significantly reduced cartilage breakdown. Delivery of the hIL-10 was less effective, When both genes were used together, there was a greater inhibition of cartilage breakdown and a considerable reduction of cartilage matrix degradation.

构建PLXRN-IL-1Ra、PLXRN-IL-10逆转录病毒载体,体外感染同种异体原代滑膜细胞,接着将转染了外源基因的滑膜细胞行关节腔注射入创伤性骨关节炎兔膝关节,通过对关节滑液的ELISA分析证明外源基因的表达在基因转移后14天还很稳定,只接受hIL-1Ra基因治疗的膝关节软骨损坏明显减轻,接受hIL-10基因治疗的膝关节治疗也有一定效果,当两种基因同时导入时,有非常明显的抑制软骨破坏和软骨基质降解的作用。

Unlike existing sensors, LaRee1 detects MMP12 activity by loss of FRET plus internalization of the lipidated fragment. In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages.

海德堡大学医学院研究人员参与的科研小组开发出一种荧光探针,可以利用荧光共振能量转移技术探测出MMP-12酶的活性,从而判断肺部是否发生病变。

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