查询词典 laccase

To screen the strains with high production of laccase from A. Auricula so that the properties and gene expression of A. Auricula laccase as well as its structures and functions can be studied.

为筛选黑木耳漆酶高产菌株以便对黑木耳漆酶酶学性质、基因表达及结构与功能进行研究，从河南省农业科学研究院、河南省生物研究所购得黑木耳菌株10株。

Four white-rot fungi, Pleurotus eryngii, Polyporus versicolor, Pleurotus ostreatus and Fomes lignosus, are tested for their abilities to produce lignin peroxidase, manganese peroxidase and laccase in N-limited and N-rich media with shake culture. The four white-rot fungi only produce manganese peroxidase and laccase. Pleurotus eryngii and Pleurotus ostreatus are selected, due to their high enzyme activities, for the degradation and decolorization of alkali lignin.

首先观察了四种白腐菌Pleurotus eryngii，Polyporusversicolor，Pleurotus ostreatus和Fomes lignosus在限氮和富氮培养基中振荡培养产木素过氧化物酶、锰过氧化物酶和漆酶的能力，由于Pleurotus ostreatus和Pleurotus eryngii产酶能力较强，用于碱木素的降解脱色效果较好，二者在限氮条件下11天对碱木素降解脱色率为65%和72%。

This study investigated the effect of Cd on remediation of DDT contaminated soil using free laccase and immobilized reversed micelles laccase.

研究了重金属Cd对游离漆酶和固定化反胶团漆酶修复土壤有机氯农药DDT污染的影响。

In laccase-ABTS mediator system, the mechanism showed that the catalyzed oxidation reaction of adrenaline consisted of four chemical equations, and the produced rate of adrenochrome depended on the rate constant of each equation and the experimental conditions such as the concentrations of laccase, substrate and mediator.

UV光谱表明，加入介体以后，肾上腺素红的生成速度明显提高。

There are three titres of laccase during the solid state ferment process of 20 days, which appeared at 6, 15 and 18 days, 7 isoforms of laccase.

在固体发酵20天过程中出现三个产漆酶高峰，分别在6天，15天和18天，最多同工酶达7种之多。

The correlation between the parameters of laccase-treated lignin and salts of lignosulphonate and the shearing strength of plywood was analyzed. Result shows that the optimum parameters of laccase-treated lignin and salts of lignosulphonate were different respectively.

研究了不同漆酶处理活化木素及其磺酸盐的工艺条件与胶合板的胶合强度之间的相关性，漆酶分别处理木素及其磺酸盐的各自最佳条件不尽相同。

The dynamic variation of laccase activity and pH were detected during fermentation of Trametes versicolor 1126. The effects of sodium carboxymethyl cellulose and phenol on laccase activity were also studied.

考察了云芝Trametes versicolor 1126发酵培养中漆酶酶活和pH的变化，同时研究了羧甲基纤维素钠及苯酚添加量对漆酶活力的影响。

Versicolor suited to grow and produce laccases at pH value 3.5-5.7, and unbuffered media were able to get good laccase productivity though buffered media could get higher laccase activity. Metal ions Cu(superscript 2+) and Mn(superscript 2+) in the culture could remarkably increase the activity of laccases. The optimal concentration of Cu(superscript 2+) was 0.008-0.08mmol/L, and the optimal concentration of Mn(superscript 2+) was about 0.01mmol/L, when the culture contained 0.1mmol/L 2,5-dimethoxyaniline.

结果发现该菌种适宜在pH值3.5~5.7环境下生长并合成漆酶，培养基中添加缓冲液可以获得更高的酶活，但不加则有较高的酶产率；铜和锰可以显著提高漆酶活力，当以0.1mmol/L 2，5-二甲基苯胺为诱导剂时，培养基中宜含0.008~0.08mmol/L Cu(上标 2+)，0.01mmol/L左右的Mn(上标 2+)。

In this thesis, the submerged fermentation and solid-state fermentation of Coriolus versicolor laccase, the enzymatic characterization, and the application of laccase in indigo and gold orange Ⅱ decolorization was investigated. The major results were as follows:The laccase production by Coriolus versicolor was performed in submerged fermentation, using corn husk as carbon source and carbamide, ammonium sulfate as nitrogen source.

本文对Coriolus versicolor漆酶的液体发酵和固态发酵进行了研究，并且对Coriolusversicolor漆酶的酶学性质及其在靛蓝、金橙Ⅱ脱色降解方面的应用进行了探讨，其研究成果如下：对Coriolus versicolor漆酶的液体发酵工艺参数进行了优化，结果表明，以玉米皮为碳源，尿素和硫酸铵为氮源，利用Coriolus versicolor液体发酵产漆酶时，C／N以20为宜。

New laccase gene and its full-length cDNA were clone from genomic library and equalized cDNA library of Ganoderma lucidum. Similarity between the sequence and other laccase gene were analyszed with NCBI blast, the results show that the new gene belong to multicopper oxidases gene family and have 60-70% Similarity with other laccase gene.

从灵芝的基因组文库和cDNA均一化文库中克隆了灵芝的漆酶基因和全长cDNA，并通过NCBI Conserved Domain Search氨基酸序列的相似性分析及与其它真菌漆酶之间的相似性分析，证明得到的蛋白是一种新的multicopper oxidases，其蛋白质序列与其它已克隆漆酶基因的同源性在6 0～7 0％之间，并对论文克隆的漆酶基因的启动子和终止子进行了分析。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。