查询词典 labeled graph

It was found that only few log-phase trophozoites were labeled by means of C6-NBD-ceramide, however, most of the encysting trophozoites were labeled in perinuclear region.

结果 极少数蓝氏贾第鞭毛虫滋养体被C6-NBD神经酰胺标记；多数处于成囊6、12、18h时段的虫体均可见被特异标记成亮绿色的核周围区域。golgin245和Rab1在滋养体及成囊诱导6、12、24h的虫体中的表达水平无显著变化。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

The main distribution area of HRP retrogradely labeled neurons overlapped with the BDA anterogradely labeled fibers and terminals within the PBN.

结果 将HRP注入VPM后，在光镜下可见HRP逆标神经元主要位于同侧的臂旁外侧核、K lliker-Fuse核和臂旁内侧核。

Results:(1) The 90Y activity was higher while the contumination of 90Sr was less than 0.0031%;(2) The efficiency of 90Y-labeled antibodies was over 90%,the activity of labeled antibody maintained 82.3%;(3) Tumor size of all 90Y-antibody-treated model was significantly less than that of all 90Y-Cl3-treated model, the average survival time of the mice of the 90Y-antibody-treated group was significantly longer than that of the 90Y-Cl3-treated group;(4) In the tissue sections extensive necrosis was observed in all 90Y-antibody-treated tumor, the living tumor cells were immunohistochemically stainable with PcNA.

结果：(1)EDTA淋洗法制备90Y纯度高，其相对含量可达99.997％；(2)单克隆抗体的90Y标记率达90％以上，抗体蛋白活性保持82.3％；(3)治疗结果表明治疗组肿瘤明显小于对照组，原肿瘤的指数生长规律遭到破坏，治疗组生存期明显长于对照组；(4)组织学切片显示治疗组肿瘤有广泛坏死，PcNA免疫组化显示治疗组肿瘤细胞增殖受到明显抑制。

Methods: RSCs were prepared from the retina of embryonic day 19 SD rats and labeled with different concentration of SPIO (Fe3+ 5.6μg/ml、11.2μg/ml、16.8μg/ml、22.4μg/ml). Prussian blue staining, electronmicroscope were used to evaluate the labeling efficiency of SPIO labeled RSCs. Cell proliferation and apoptosis were quantified by FACS, MTT and Turnel.

来源于19d胚胎大鼠的视网膜前体细胞，以不同浓度SPIO标记(Fe3+颗粒浓度为5.6mg/L、11.2mg/L、16.8mg/L、22.4mg/L)24～48h后，进行形态学观察和流式细胞技术、MTT和Turnel等技术分析，研究细胞增殖和凋亡情况。

The primary difference is that in RIA systems the competitive antibody is labeled with a radioisotope and in FIA the antigen is labeled with a compound that fluoresces under the appropriate light rays.

主要不同的是在放射免疫测试法中，竞争性抗体是用放射性同位素标记；在免疫荧光测试法中，用一种在适当光波长下能发出荧光的物质。

In the LAB, the solid phase first, and non-biotin labeled avidin reaction, and then coupled with biotin-labeled enzymes to further enhance the sensitivity.

在LAB中，固相生物素先与不标记的亲和素反应，然后再加酶标记的生物素以进一步提高敏感度。

Its principle is as follows:red blood cells are labeled with NHS-Biotin,participated in the circulation and ages in vivo.Using the high affinity between biotin and avidin,the labeled RBCs with specific ages are separated.

其基本原理是用生物素标记一定量的红细胞，使之参与血液循环，并在体衰老，利用biotin与抗生物素蛋白之间的高亲和力，分离带有标记的、具有特定年龄的红细胞。

In this study, in order to examine the dynamics of tip [Ca2+]i together with the dynamics of tip-localized F-actin in vivo, a kind of double labeled material was constructed. The Ca2+ and actin microfilaments of Arabidopsis pollen tubes were labeled by cameleon and GFP-mTalin respectively.

本研究以拟南芥花粉管为材料，通过转基因技术，将分别标记Ca~(2+)和微丝的两种荧光蛋白cameleon与GFP-mTalin在花粉管中同时表达，实现活体花粉管中Ca~(2+)与微丝的同时标记。

After two rice-wheat rotations, 11%–13% of applied labeled N remained in 0-60 cm soil (mostly in the 0-20 cm soil); leaching of labeled urea applied in rice season was little (only 0.6%–1.1% of the applied N accumulated in 20-60 cm soil), and the leaching of fertilizer N occurred in wheat season and the beginning flooding period of the next rice season; 47%–54% of applied fertilizer N was lost and ammonia emission and nitrification-denitrification were the main pathways.

水稻季施用的肥料氮向耕层以下移动很少，20—60 cm土层中累积肥料氮仅占施氮量的0.6%～1.1%，主要发生在小麦季及水稻泡田时期，肥料氮损失占施氮量的47～54%，氨挥发和硝化反硝化气态损失是主要途径。

We got alerted a couple of times while we were down south that HETs were on the way to bring us back up north because things were going to go hot again, but it was just rumors.

南下的途中我们不只一次得到警告说重型装备运输车将拉着我们重新北上，因为局势正在变得重新紧张起来，但这只是谣传。

It's the one where they find the ghost in the salt mine.

这一集是演他们在盐矿找到鬼