查询词典 labeled graph

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只，每组再分为高代谢脓毒症组和对照组两部分，建模方法同第一部分；2）三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸；3）灌注前抽取动脉血作为背景值，灌注达150，160，170和180min分别抽取动脉血2ml用于质谱分析；4）实验开始后前30min中每5min收集呼出气一次，随后每30min收集呼出气一次，用于气相质谱分析；5）血样经过低温离心取上清液，过阳离子及阴离子交换树脂，再经衍生化处理；6）处理过的血样进入气相色谱－质谱仪，测量其13C标记的亮氨酸、葡萄糖和乳酸丰度；7）实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度（E13CO2）；8）利用文献提供的公式算出CO2总生成率（V13CO2）以及机体各物质的通量、氧化速率以及物质代谢百分比等；9）数据分析处理采用t检验。

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

In fetal, otocyst cells proliferated and differentiated and showed several morphotypes, which labeled positive to F-actin and cytokeratin. A subset of cells were labeled by calretinin and BrdU.

4100ng/ml和200ng/ml bFGF二组中calretinin阳性着染的细胞数量分别占所观察的细胞总数的7％和7.6％，1ng/ml和10ng/ml bFGF组中，calretinin阳性着染的细胞数量分别为0.5％和1.6％。

To the problem that large-scale labeled samples isn't easy to acquire in the course of SVM training, the active learning strategy is used in the SVM training and an incremental training algorithm of active SVM based on the uncertainty based sampling of distance ratio is proposed in the paper. The experimental results show that the active SVM learning strategy can considerably reduce the labeled samples and costs compared to the passive learning method, and at the same time it can ensure the accurate classification performance is kept as the passive SVM and also expedite the SVM training.

针对 SVM训练学习过程中难以获得大量带有类标注样本的问题，本文将主动学习策略应用于SVM增量训练中，提出了一种基于距离比值不确定性抽样的主动SVM增量训练算法，实验结果表明在保证不影响分类精度的情况下，应用主动学习策略的SVM选择的标记样本数量大大低于随机选择的标记样本数量，大大降低了标记的工作量或代价，而且训练速度同样有所提高。

Then the labeled Hela cells were implanted into the subcutaneous tissue of nude mice and the growth of the implanted tumor was observed and growth curve of the labeled Hela cells was analyzed with the whole-body optical imaging system and the slide caliper.

BALB/cA-nu裸鼠皮下接种1×106个发光细胞使其成瘤，利用活体荧光成像系统和游标卡尺观察肿瘤的生长情况。

Gold colloids were labeled with Raman reporter mercapto molecules such as 4-mercaptobenzoic acid and thiophenol to form Fourier transform surface-enhanced Raman scattering reporter-labeled gold colloids.

以对巯基苯甲酸、苯硫酚为标记分子，与金纳米粒子生成具有SERS信号的标记金溶胶。

Proteins associated with the membrane and the cytosolic portions of the proteome under different copper ion concentrations were differentially labeled with an acid cleavable isotope-coded affinity tag reagent, and the labeled peptides were separated by two-dimensional chromatography and characterized by electrospray ionization-tandem mass spectrometry.

我们分别将在不同铜离子浓度下的细胞质以及细胞内膜中所有蛋白质标识上酸裂性同位素亲和性标识(acid cleavable isotope-coded affinity tag， cICAT)试剂，用二次元管柱层析将所有标识并酵素水解过的片段分离，并且用电洒式串连质谱(electrospray ionization-tandem mass spectrometry， ESI-MS/MS)以及基因体定序资料库决定蛋白质序列。

Therefore, in the present study, a retrograde and anterograde experiments were made in the rat to address this issue. After injection of cholera toxin B subunit, a retrograde tracer, into Barrington's nucleus or D-region, some retrogradely labeled neurons were found in lamina I, sacral parasympathetic nucleus and dorsal commissural nucleus of the lumbosacral spinal cord segments. Injection of biotinylated dextran amine into lamina I, sacral parasympathetic nucleus and dorsal commissural nucleus, respectively, a large number of BDA-labeled axon terminals were seen in Barrington's nucleus and D-region.

发现，将逆行示踪剂霍乱毒素 B 亚单位（ Cholera toxin B subunit； CTb ）注射到 Barrington's 核或 D 区后，在大鼠腰骶髓 I 层、骶髓副交感核和后连合核内分布有一定数量的 CTb 逆标神经元；将顺行示踪剂结合生物素的葡聚糖胺(Biotinylated dextran amine； BDA)分别注射到腰骶髓 I 层、骶髓副交感核和后连合核内后， Barrington's 核和 D 区内出现大量顺行标记的轴突终末。

All of them were labeled with [32P] orthophosphate (2.03×10^5 Bq/mL) for 2 h and then stimulated with CCK8 l0^(-7 mol/L and DMEM respectively for different time. Reactions were terminated by freezing neurons in liquid nitrogen. The 32P-labeled lysates isoelectrically focused on IPG Drystrip pH 4~7 linear gels and subsequently separated by second-dimensional SDS-PAGE.

培养乳小鼠神经元，随机分为CCK8刺激组和对照组，32P-NaH2PO4标记后分别给予CCK810^(-7 mol/L和等量无磷培养基，作用不同时间后液氮终止反应，经双向电泳、放射自显影，获得磷酸化蛋白质的电泳图谱。

Methods: the spiral modiolar artery in guinea pig cochlea was exposed by microsurgery, then the fluorogold or horseradish peroxidase was given around the sma for retrograde tracing. the bilateral superior cervical ganglia and stellate ganglia were observed to find retrogradely labeled neurons. the neurotransmitters of these sympathetic fibers around sma were ascertained by using retrograde tracing combined with immunohistochemical double labeled technique.

在正常豚鼠单侧耳蜗螺旋蜗轴动脉局部滴加逆行追踪剂辣根过氧化物酶或荧光金，观察双侧交感颈上神经节、星状神经节内hrp或fg逆标神经元的分布；采用逆标与免疫组化相结合的双重标记方法观察追踪阳性神经元是否表达酪氨酸羟化酶、神经肽y、钙基因相关肽或p物质。

We got alerted a couple of times while we were down south that HETs were on the way to bring us back up north because things were going to go hot again, but it was just rumors.

南下的途中我们不只一次得到警告说重型装备运输车将拉着我们重新北上，因为局势正在变得重新紧张起来，但这只是谣传。

It's the one where they find the ghost in the salt mine.

这一集是演他们在盐矿找到鬼