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Results The IC50 of SPG-Rg3 was (155.7±0.71) mg·L-1,when the concentration of SPG-Rg3 was arranged from 37.5 to 600.0 mg·L-1,the growth inhibitory rate of MCF-7 cells was higher following the increase of its concentration,the cell growth was greatly inhibited compared with control group(P.05); cell cycle was changed,the cell number of S period increased compared with control group(P.01),the cell number of G2/M period decreased compared with control group(P.01),in experiment group there was an apoptotic apex before G1 apex,and the number of apoptotic cells increased; most of cell nuleus in SPG-Rg3 (150 mg·L-1)experiment group appeared yellow or chrysoidine fluorescence,contracted,beaded and crescent; the result of immunocellularchemical staining of caspase-8 indicated that caspase-8 protein had no expression in control group,but stronger expression in SPG-Rg3 group.

结果：SPG-Rg3 IC50为（155.70±0.71） mg·L-1，当SPG-Rg3浓度在37.5~600.0mg·L-1时，MCF-7细胞的生长抑制率随SPG-Rg3浓度的增加而增大，与对照组比较，细胞增殖受到明显抑制（P.05）；MCF-7细胞的生长周期也发生变化，S期细胞数增加，明显高于对照组（P.01，G2/M期细胞数则明显少于对照组（P.01；并且在G1峰前出现明显的凋亡峰，凋亡细胞数增加。

The germinative number of horsebeans and peas were raised by 44% with activated water by tourmaline. 200μl 1 X 10~6/ml mouse's H_(22) cell, 200μ 4X 10~6/ml splenic cell, 200μl 1 × 10~6/ml epidermal cell were all inhibited after the action of tourmaline. It is analyzed that the factors which effected the multiplication of the cells included direct action which are tourmaline's electric field, far-infrared, negative ions and solvent ions, and indirect effect which are small water clusters and pH value.

电气石活化水使蚕豆和绿豌豆种子发芽速度均提高44%。0.5g电气石分别对200μl 1×10~6/ml的小白鼠H_(22)肝癌细胞，对200μl 4×10~6/ml小白鼠脾细胞，对200μl 1×10~6/ml的小白鼠表皮细胞的增殖起抑制作用，分析认为，电气石影响细胞增殖的因素包括电场、红外线、负离子和溶出离子对细胞的直接作用和通过改变水分子结构、培养基pH值的间接作用。

Results ⑴ The cell proliferation was inhibited by TET. It showed thatdivision index and cell number during G0-G1 were decreased, while S and G2-M phases were lengthened with dosage and time dependency in dosages of TET from 2mg/L to 8mg/L; besides, oval-shap cells, karyopyknosis and fragmentation were observed under light microscope. The inhibitory effect in T2 group was statistically similar to that in triamcinolone.

结果⑴ TET在2mg/L~8mg/L之间呈剂量及时间依赖性地抑制瘢痕成纤维细胞的增殖，使生长曲线不同程度的压低、分裂指数降低、G0-G1期细胞数减少、G2-M期增多、S期与G2-M期延长，光镜下见细胞突起变短，细胞由梭形变成椭圆形，核固缩、碎裂。P.C的改变近似于T2组(4mg/L TET)。

The greatest efficiency in Agrobacterium-mediated transformation of lettuce cotyledons(2～3 days-old) was obtained by 10-time dilution of Agrobacterium cells, 15-min inoculation time,1-day pre-culture,4-day co-culture and without AS using the MS(Murashige and Skoog\'s,1962) medium supplemented with 0.2 mg/L 6-benzylaminopurine,0.1 mg/Lα-naphthaleneacetic acid,100 mg/L Kan and 500 mg/L Carb,.

在农杆菌介导的皱叶生菜子叶(2～3 day苗龄)转化试验中，采用含BA 0.2mg/L，NAA 0.1 mg/L，Kan 100 mg/L，Carb 500 mg/L的MS培养基，最优的转化条件为农杆菌稀释倍数为10倍，预培养时间为1 day，共培养时间为4 day，浸染时间为15 min，不添加乙酰丁香酮。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

The results indicated that,in vitro,the L-glutamate of different concentrations(2.9～11.7 mmol*L-1)was capable of remarkedly increasing and MT(1～10 mmol*L-1) added together with L-glutamate was capable of remarkedly reducing the level of MDA in homogenates of the rat′s whole brain; in vivo,the L-glutamate of different doses(50～200 μg*mice-1)were injectedi.c.v.

结果表明，在体外，不同浓度的谷氨酸(2.9～11.7 mmol/L)均能显著提高大鼠脑匀浆中丙二醛的含量；而加入MT(1～10 mmol/L)，可显著的对抗这种升高。

Finally indicated, the best seed raise conditions of T11-1 and O7-7 is: 100mL seed culture mudium in 200 mL triangle bottle ,16 hours in shake bed;It′s best component fermentation medium is: peptone 5.0,yeast extract 3.0, beef paste 2.0,xylose 60.0,K_2HPO_4 2.0,MnSO_4·4H_2O 0.04,MgSO_4·7H_2O 0.20,FeSO_4·7H_2O 0.05;The best fermentation condition is: 150mL fermentation medium in 250mL bottle,vaccinates 10 %, 48 hours in shake bed under 46℃;The output of L-lactic acid using T11-1 and O7-7 from corncob respectively were 31.30g/L and 30.88g/L,and the conversion rate respectively were 52.17% and 51.47%.

结果表明，T11-1和O7-7的最佳种子培养条件为：在200mL三角瓶中于盛装100mL种子培养基，摇床培养16h；其最佳发酵培养基组分为：蛋白胨5.0，酵母浸粉3.0，牛肉膏2.0，木糖60.0，MnSO_4·4H_2O 0.04，MgSO_4·7H_2O 0.2，FeSO_4·7H_2O 0.05，K_2HPO_4 2.0；最佳发酵条件为：250mL输液瓶装载150mL发酵培养基，接种10%，在46℃条件下摇床发酵48h；利用T11-1和O7-7进行玉米芯L-乳酸发酵，其L-乳酸产量分别为：31.30g/L和30.88g/L，转化率分别为52.17%和51.47%。

The optimum condition for separating and purifying SAM was using the solution at pH of 5.0 containing 3.0 g SAM per liter at the flow velocity of 6 L/h through the ion-exchange column (φ50 mm×1500 mm)filled with D152 resin. Impurities were removed first by eluting the column with double-distilled water and acetic acid, and then with 9 L to 15 L of sulfuric acid of 50 mmol/L concentration at the flow velocity of 6 L/h.

论文对SAM的性质、药理作用以及当前国际上关于SAM的研究进展进行了较为详尽的综述，并在此基础上确立了研究内容，取得了如下成果： 1利用本实验室构建的基因工程菌JM71发酵生产S-腺苷-L-蛋氨酸，在50 L发酵罐中发酵6天后，SAM终产量达到6 g/L发酵液。

Potassium channel blockers(TEA10mmol/L and 4-AP10mmol/L) partly reduced the majority of outward current to 23.4±2.4%.(2)Bath application of Cl—-free Ringer's solution and potassium channel blockers(TEA10mmol/L and 4-AP10mmol/L) could almost completely block the outward current, only remaining 2.2±0.2%.

该电流被最大抑制剂量的钾离子通道拮抗剂TEA(10mmol/L)和4-AP(10mmol/L)协同作用时，只能被抑制到最大电流幅度的23.4±2.4%；(2)用无氯Ringer氏液与钾离子通道拮抗剂(TEA10mmol/L和4-AP10mmol/L)协同作用时，可将此电流基本抑制，仅剩2.2±0.2%。

L( r" s# Z: X2 p) Z- Q0 c- r If you are planning to run in Sysop mode, put an -R or -L at the end of your call to indicate a "repeater" or "link, respectively (for example, K1RFD-L). Use -L to denote a simplex link, or -R if the link is tuned to the frequency pair of a local repeater.

如果用户计划使用系统操作模式，则需要在呼号后加L或R，以表示连接或中继（例如：BG1KLS-L）。L代表直发连接，而R代表用户将Echolink的连接调到本地中继相同的上下行频率。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失