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Under the optimum conditions, the analytes could be separated within 17 min in a 75 cm length capillary at a separation voltage of 18 kV in a 10 mmol/L borate buffer (pH 8.2). A 300 μm diameter carbon disk electrode generated a good response at+ 0.95 V vs. saturated calomel electrode (SCE for all analytes. The relationship between peak currents and analyte concentrations was linear over about three orders of magnitude with detection limits (S/N=3) ranging from 78 μg/L to 110 μg/L for all analytes.

在优化的实验条件下，以直径300μm的碳圆盘电极为工作电极，检测电位为＋0.95V，在10mmol/L硼酸盐(pH8.2)的运行缓冲溶液中，上述6种活性成分能在17min内实现很好的基线分离，被测物浓度与峰电流在3个数量级范围呈良好的线性关系，检出限(S/N=3)范围为78~110μg/L。

The linear ranges all were 5~200 mg/L and the detection limits (S/N=3) were 0.20 and 0.15 mg/L for arsanilic acid and sulfanilic acid, respectively.

阿散酸和对氨基苯磺酸的线性范围均为5～200 mg/L，检出限(S/N=3)分别为0.20 mg/L和0.15 mg/L。

While two herbicides had no significant effects on the growth of three cyanobacteria at low concentrations (0.01~0.001mg/L), they made damage to cells and affect cyanobacteria's growth at high concentrations (10~100mg/L). EC_(50) values of three cyanobacteria were showed below: Anabaena azollae, 3.172 mg/L; Anabaena azotic, 3.654 mg/L and Anabaena flos-aquae, 3.377mg/L Nitrogenase activities of three species also showed different tolerant capabilities to the two herbicides under 10% vol.

在低浓度(0.01~0.001mg/L)的2种除草剂的作用下，3种固氮蓝藻的生长没有受到明显的影响，而在高浓度下(10~100mg/L)，固氮蓝藻藻细胞在形态上出现受损症状，同时生长速率也受到明显的抑制。120h时，满江红鱼腥藻，水华鱼腥藻及固氮鱼腥藻的EC_(50)值分别为3.172， 3.654及3.377mg/L。

And it is proved that a semiring S in ■°■l is a subdirect product of a member of ■° and a member of ■l if and only if the multiplicative reduct of S is a subdirect product of a group and a semilattice by using the concept of sturdy frame of type(2,2) algebras.

O∩■°■B和■∩■°■中成员的次直积分解，并利用&(2，2)型代数的坚固构架&的概念，证明了半环S∈■°■l是■与■l中成员的次直积当且仅当S的乘法半群是群与半格的次直积。

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ES cells were maintained on gelatin-coated dishes in Iscove's modified Dulbecco medium containing 0.1 mmol/L of nonessential amino acids, 2 mmol/L of L-glutamine, 0.1 mmol/L of monothioglycerol, 15% fetal bovine serum, and 1000 U/mL leukemia inhibitory factor. Embryonic bodies were firstly formed by ES cells with hang drip method in IMDM without LIF. Six-day-old EBs were trypsinized and the cells were resuspended at a concentration of （3-4）×109 L-1 cells per 1 mL of a 1:1 mixture of culture medium and Matrigel?

实验方法：将鼠胚胎干细胞株接种于被覆明胶的培养瓶内，加入含0.1 mmol/L非必需氨基酸、2 mmol/L L-谷酰胺、0.1 mmol/L二羟基丙硫醇、15%胎牛血清、1 000 U/mL白血病抑制因子的IMDM培养液。3 d后采用&悬滴法&使细胞在不含白血病抑制因子的上述IMDM培养液中形成胚胎体，6 d后胰酶消化得到胚胎干细胞，浓度调整为（3~4）×109 L-1，与Matrigel？

The results showed S curve for effects of NaCl stress on germination of watermelon seed, the middle point between two inflexions of the curve was at 120mmol/L and could be used as referenced screening concentration to study 'ZQK0395' salt tolerance genetics, the second curve inflexion was at 180mmol/L and could be used as referenced concentration for 'ZQK0395' salt-tolerant germplasm selection.

结果表明，NaCl胁迫对种子发芽的影响呈S型变化曲线，120mmol/L为S型曲线两个拐点中间值，可作为该种质资源耐盐性遗传研究的参考鉴定浓度，180mmol/L为S型曲线的第二个拐点，可作为该种质资源耐盐性参考筛选浓度。

Separated cultures were isolated from 4 different koumiss examples, the complete 16SrRNA gene of these strains were sequenced, and phylogenetic trees were constructed. The isolateswere identified to the most related strain which had the highest affinity. The identified strains weresix L. lactis, five L. plantarum, five E. faecium, four E. faecalis, two L. casei, two L. helveticus, twoS. thermophilus, one L. delbrueckii subsp, bulgaricus, one Leu. mesenteroides, one Leu. garlicum.

本研究中从4份酸马奶中分离得到了29株乳酸菌，测定了这些乳酸菌的16S rRNA基因全长序列，并构建了乳酸菌属的系统发育树，根据亲缘关系对乳酸菌进行了鉴定，其中L.lactis(6株)，L.plantarum(5株)，E.faecium(5株)，E.faecalis(4株)，L.casei(2株)，L.helveticus(2株)，S.thermophilus(2株)，L.delbrueckii subsp.bulgaricus(1株)，Leu.mesenteroides(1株)，Leu.garlicum(1株)。

Bath application of chloride channel blocke(rNPPB30μmol/L)and potassium channel blockers(TEA10mmol/L and 4-AP10mmol/L) could almost completely block the outward current, only remaining 2.1±0.2%.Bath application of Ca2+-free Ringer's solution and potassium channel blockers(TEA10mmol/L and 4-AP10mmol/L) could almost completely block the outward current, only remaining 3.2±0.3%.

用氯离子通道拮抗剂NPPB(30μmol/L)与钾离子通道拮抗剂(TEA10mmol/L和4-AP10mmol/L)协同作用时，该电流也可被基本抑制，只剩2.1±0.2%；(3)用无钙Ringer氏液与钾离子通道拮抗剂(TEA10mmol/L和4-AP10mmol/L)协同作用时，可将此电流基本抑制，仅剩3.2±0.3%。

The optimal medium components were: sodium alginate 3 g/L,(NH4)2SO4 3 g/L, NaCl 20 g/L, KH2PO4 0.1 g/L, CaCl2 0.1 g/L; The optimal culture conditions were: 25 ml medium in 250 mL Erlenmeyer flask, inoculum's volume 3%, shaking speed of 150 r/min, initial pH 7.5, at 28°C for 24 h.

该菌株产酶的最佳培养基组成为：褐藻胶3 g/L、(NH4)2SO4 3 g/L、NaCl 20 g/L、KH2PO4 0.1 g/L、CaCl2 0.1 g/L；最佳培养条件为：250 mL三角烧瓶中装液量25 mL、接种量3%、摇瓶转速150 r/min、pH7.5、培养温度为28℃、培养时间为24 h。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失