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Result］ The new buds and petals of I.germanica was proper to be used as explant of tissue culture, with lower browning rate compared with other positions. The well situated tissue block of explant (3 mm×3 mm×3 mm) might reduce browning rate. Adding 0.1%Vc or 0.3% active carbon in medium had obvious effect on preventing tissue browning. The exogenous hormone 2 mg/L 6-BA was easy to lead to brownness turning, and 2 mg/L Kt and 2 mg/L NAA brought less effect to tissue browning. When using disinfectants of alcohol, its dipping time of 5～10 s for explant was the best, with the minimum browning rate. The culture under full illumination condition was liable to induce brownness turning, compared with other cultured conditions, the brownness turning could be restrained effectively by using imitating artificial climate condition for culture.

结果］ 鸢尾的新生芽和花瓣较适宜作组织培养的外植体，与其他部位相比褐变率较低；外植体组织块适中（3 mm×3 mm×3 mm）可降低褐变率；在培养基中加入0.1%Vc或0.3%活性炭对防止组织褐化效果明显；外源激素2 mg/L 6-BA易引发褐变，2 mg/L Kt和2 mg/L NAA对组织褐变影响不大；使用乙醇消毒剂时，对外植体浸泡时间以5～10 s为佳，褐变率最低；全光照条件培养容易诱发褐变，采用仿人工气候条件培养较其他条件培养可有效抑制褐变的发生。

Diffusion coefficients for metol and p-benzenediol were 2.1×10 cm^2/s and 2.7×10^6 cm^2/s, respectively. The electrocatalysis mechanism has been studied. ATR-FTIR was used for the characterization of the ME/Au SAMs. The oxidation currents were linear in the range of 1.0×10^(-7)~1.0×10^(-4)mol/L for metol and 6.0×10^(-6)~6.0×10^(-4)mol/L for p-benzenediol, with the correlation coefficients of 0. 9992 and 0.9996 by differential pulse voltammetry, and detection limit 2.0×10^(-8)mol/L and 1.2×10^(-6) mol/L for metol and p-benzenediol, respectively.

采用水平衰减全反射－傅立叶变换红外光谱技术对ME/Au SAMs进行了表征，示差脉冲伏安法同时测定米吐尔和对苯二酚，不产生干扰，其氧化峰电流与相应物质的浓度分别在1.0×10^(-7)~1.0×10^(-4)mol/L和6.0×10^(-6)~6.0×10^(-4)mol/L范围内呈良好的线性关系，相关系数分别为0.9992和0.9996，检测限分别为2.0×10^(-8)和1.2×10^(-6)mol/L。

Results: The mean concentration and standard error of Fe. Ca, Zn, Cu. Mg and Pb in the blood of all subjects were 8.30±0.79μmol/L, 1.59±0.14 mmol/L, 70.68±9.45μmol/L, 15.56±3.42μmol/L. 1.68±0.14 mmol/L and 0.354±0.130μmol/L, respectively. Amount of children occurred the deficency of Ca, Zn and Fe and plumbism.

结果：以平均值±1倍标准差的形式来表示所有儿童全血微量和铅的测定结果如下(平均值x±s：Fe，(8.30±0.79)μmol/L；Ca，(1.59±0.14)mmol/L；Zn，(70.68±9.45)μmol/L；Cu，（15.56±3.42μmol/L；Mg，(1.68±0.14)mmol/L；Pb，(0.354±0.130)μmol/L。

The calibration curve of the SM2-Kit with standard SM2 inhibitor was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 0.625 μg/L to 80.0 μg/L (R2=0.9911), the sensitivity of 0.9 μg/L, the IC50 of 5.82 μg/L and the detection limit of 1 μg/L. The recoveries of SM2 spiked in pig feed were 83.4 %, in pig urine were 89.5 %. The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation was both below 15 %. The SM2-Kit generally had 2.08 % cross-reactivity towards sulfamerazine and little or no cross-reactivity towards other sulfonamides. The dilution solution of SM2 had no effect on results of SM2-Kit. The validity of SM2-Kit in 4 ℃ was above six months.

研究结果表明，SM2-Kit的标准曲线呈典型的S型，相关系数R2=0.9911，符合4参数logit曲线拟合，线性检测范围为0.625 μg/L～80 μg/L，灵敏度为0.9 μg/L，半数抑制浓度(IC50)为5.82 μg/L，检测限为1 μg/L；饲料样、猪尿样的平均添加回收率分别为83.4 %、89.5 %，平均批内和批间变异系数均低于15 %；SM2-Kit与磺胺甲基嘧啶的交叉反应率为2.08 %，与其它磺胺类药几乎没有反应性；基质对SM2-Kit的检测结果影响不大；试剂盒在4 ℃可保存6个月。

The linear concentration ranges for γ-aminobutyric acid, valine and leucine were 5.0×10^(-3)-0.12 g/L, 5.0×10^(-3)-0.08 g/L and 5.0×10^(-3)-0.12 g/L, respectively, and the detection limits (S/N=3) were 2.5×10^(-3), 2.5×10^(-3) and 2.6×10^(-3) g/L, respectively. The relative standard deviations of the peak currents of γ-aminobutyric acid, valine and leucine were 2.5%, 4.9% and 3.9%(n=7), respectively.

氨基丁酸、缬氨酸和亮氨酸的线性范围分别为5.0×10^(-3)~0.12、5.0×10^(-3)~0.08和5.0×10^(-3)~0.12 g/L，检出限(S/N=3)分别为2.5×10^(-3)、2.5×10^(-3)、2.6×10^(-3)g/L；7次平行进样峰高的相对标准偏差分别为2.5%、4.9%、3.9%。

The results show that the duration of the 3 phases during the culture of cell suspension is related to the inoculum size, the initial nutrient concentration, the feeding of nutrients and the culture conditions such as temperature and dissolved oxygen, that the maximum cell yield reaches about 12g/L for batch culture or 35g/L for fed-batch culture in dry weight, that the saponin content of cells quickly increases in the bloom phase and keeps on increasing until the early stage of the declining phase, with a maximum saponin content of about 60~70g/kg in dry weight, that the conductance value of cell suspension decreases from more than 8000μS to less than 600μS with the growth of cells, but increases when the cells autolyze, and that part of cells turns red in the later culture, which has no apparent effect on the saponin content.

结果显示：细胞3个生长时期的持续时间与接种量、初始营养浓度、营养流加情况及培养条件有关；分批培养和流加培养时的最大细胞得率分别为12g/L和35g/L；细胞的皂甙含量在旺盛生长期快速上升，并保持增长至衰退期前期；干细胞中最大的皂甙含量达60~70g/kg；随着细胞的生长，培养液的电导值从8000μS以上下降至600μS以下，细胞自溶后逐渐回升。另外，培养后期部分细胞颜色变红，但没有发现颜色转变对皂甙含量造成影响。

H.伸缩环转动环伸缩环垫片前轴封 S D / L D / S L M E C H A N I C A L S E A L P U M P S SD/LD/SL SERIES PUMPS The SD/LD/SL series are dependable, self priming pumps. The full motor horsepower is translated into fow via the shaft. These pumps are used for a wide range of applications, from chemical transfer to process solution recirculation to waste treatment. This series is capable of self-priming up ro 20 feet of lift. The mechanical seal is a self-cooling, Tefon bellows which is cooled by the solution being pumped. The construction materials and seals avaiable make this pump extremely versatile. The horizontal design has a working temperature from167℉ to 194℉(75℃ to 90℃), depending on the material of construction, and can handle most chemical solutions. The SD/LD/SL series pumps are designed to run outside the tank or process module. The SD/LD series pumps incorporate a FRPP molded base, legs and pump-to-motor casing. There are no bolts or metallic parts in contact with the solution being pumped.

SD/LD/SL泵是可靠的自吸泵，马达全部的马力经由轴心完全转化为流量，此型泵广泛地使用於从化学药水循环或加工溶液循环到废水处理等各种用途，此型泵可自吸达20英呎，机械轴封是自我冷却的，经由抽送的溶液来冷却，可供应的不同结构材质及轴封使此种泵的用途非常多样化，依其结构材料的不同，其卧式设计的工作温度由75℃到90℃(167℉到194℉)，而且可处理绝大部份的各种溶液，SD/LD/SL是设计用於槽外或加工模组之用，SD/LD加入一个FRPP射出成型的脚座，脚架及泵到马达的外罩，没有螺栓或金属会接触到所抽送的溶液。

The results calculated by the Elovich rate equation showed that K release rates from 1 mol.L-1 HNO3 extraction ＞those from 2 mol.L-1 HNO3 extraction ＞those from H-resin extraction. Elovich equations combining with the data of H-resin and 2 mol.L-1 HNO3 successive extraction could well described the release characteristics of soil K. The correlation coefficients of the equations reached most significant levels (r＝0.956**—0.999**), and standard errors of specific K release capacities between the vaules calculated by the equations and those measured by both successive extractions were smaller(s=1.61 and 3.52). The specific release rates of soil K calculated by the Elovich rate equations were significantly correlated with the indices of soil K availability (the amounts of easily available K, nonexchangeable K, total available K in soils and K absorbed by ryegrass).

研究结果表明：1 mol.L-1 HNO3提取法的释K速率＞2 mol.L-1 HNO3提取法的释K速率＞氢饱和交换树脂提取法的释K速率；氢质交换树脂和2 mol.L-1 HNO3提取法结合Elovich模型较理想地描述了土壤K素的释放特性，拟合方程的相关系数均达极显著水平(r＝0.956**-0.999**)，土壤特征累积释K量的计算值与实测值的标准差较小(s=1.61和3.52)，且通过速率方程计算的土壤特征释K速率与K素有效性指标(速效K、缓效 K、有效K及黑麦草总吸K量)的相关性也较为密切。

In pacemaker cells of SA node superfused with the oxygenated K—H solution, MDP, APA, V〓, VDD, RPF and APD〓 were—45±5mv, 48±4mv, 2. 4±0.9v/s, 89±8 mv/s, 200±11bpm and 206±15ms, respectively. Anoxia (perfused for 20min with a modified K—H solution gassed with 100% N〓 and deprived of glucose) increased MDP, APA and V〓, and decreased VDD and RPF in a timedependent manner. At anoxia for 20min, the above parameters were—58±5mv, 64±5mv, 11. 7±2. 7v/s, 24±7mv/s, 92±11bpm and 179± 14ms, respectively. ADase (10U/L).

环戊腺苷和苯基异丙基腺苷不仅能增加豚鼠窦房结起搏细胞APA、MDP和〓，且能减小VDD、RPF和〓在环戊腺苷20nmol／L浓度时，MDA、APA和〓分别增值5.4±0.7mv、7.6±0.9mv和2.4±0.5v／s，VDD、RPF和〓分别降低32±4mv／s、68±9bpm和31±12ms（p.05或0.01）。0.1μmol／L 8—苯茶碱和10μmol／L格列苯脲能明显地抑制环戊腺苷的电生理效应。

Results The detection limits of formic ion (S/N= 3) were 0.0705mg/L and 0.0477 mg/L with these two eluents respectively.

结果〕甲酸根离子的检出限（S/N=3）分别为0.075mg/L和0.0477mg/L，在 3μs量程档，线性范围为0.1～8.0mg/L，样品的加标回收率在92.9％-106.3％之间，RSD.0％。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失