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Firstly, callus induction and subculture for Mikania micrantha were studied systematically. The research on callus induction showed that: Shoot tip has higher induction rate than other explants ; At 25℃ and dark regime, the callus was better cultivated on Murashige and Skoog medium (pH5.8) containing 2.0mg/L 2,4-D and 1.0mg/L KT.

首先，对微甘菊愈伤组织诱导和继代增殖培养进行系统研究，愈伤组织诱导研究表明：外植体中芽尖诱导率最高，在含2，4-D2.0mg／L、KT1.0mg／L、pH5.8的MS培养基上25℃黑暗条件下愈伤组织诱导效果较好。

And the conditions in cell suspension culture of Mikania micrantha were studied. The results showed that sucrose was the compatible carbon sucrose, and 30g/L sucrose concentration can satisfy the growth of Mikania micrantha cell; ammonium was absorbed under different sucrose concentration that haven't demonstrated significant specificity, and was completely absorbed on the lag phase and the early logarithmic phase; while nitrate was mainly absorbed on logarithmic phase. The density-dependent of Mikania micrantha cell starting to grow and density-inhibited of cell growth were proposed, the fittest inoculating quantity of Mikania micrantha in cell suspension culture was 40g/L.

并对微甘菊细胞悬浮培养条件进行研究，结果表明，微甘菊细胞生长适宜的碳源是蔗糖，并且较合适的蔗糖浓度为30g／L；在不同蔗糖浓度下氨基氮的吸收差别不大，氨基氮在迟滞期和对数期前期已基本消耗完毕，对数期生长主要利用硝基氮，因而提出，在微甘菊细胞液体悬浮培养中，氮源可以采用初始低氨基氮浓度，对数期中间维持高硝基氮，而且对数期中逐步流加少量的氨基氮；微甘菊细胞迟滞期分裂启动存在密度依赖现象，对数期生长存在密度抑制现象，最适接种量为40g／L。

PCC6803. In the enclosed photobioreactor, after 58. 5h mixotrophic cultivation, 2. 50g/L cell density, 1. 0g/L. d productivity, 15. 3μg/ml chlorophyll concentration and 25. 3%energy efficiency were achieved, which were respectively 8. 9 times, 11times, 2. 3 times and 4. 9 times as much as those in photoautotrophic culture.

在封闭式光生物反应器中，混合营养培养58.5小时，集胞藻6803藻细胞密度达到2.50g/L，是同期光自养的9倍，藻细胞产率为1.0g／L.d，是光自养培养的11倍，叶绿素浓度为15.3μg／ml，是光自养的2.3倍，能量得率为25.3％，是光自养培养过程的5倍。

Detection the early stage apoptosis cells by flow cytornetry Incubating SCL-1 cells in 6-well culture medium with 1×10~6/well, after 12～48hculture with the concentration of imiquimod 0μg/mL and 150μg/mL, gathering cellsgrowing along the wall and making monocellular suspension, washing in PBS for 2times, and then add 5μL Annexin V/FITC and 10μL PI solutions, reaction for 15min avoiding light, detection the early stage apoptosis cells by flow cytometry.

流式细胞仪测定细胞早期凋亡 SCL-1细胞以1×10~6个／孔接种于6孔培养板中，使用含0μg／mL、150μg／mL咪喹莫特的培养液培养12～48h，收集贴壁生长的细胞制成单细胞悬液，PBS洗两次，分别加入5μL Annexin V／FITC和10μL浓度20μg／mL的PI溶液，混匀后室温避光孵育15min，流式细胞仪检测，结果用Cell Quest软件分析。

METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

无菌状态下取出胎儿肝脏，以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液，分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心，吸取界层细胞，添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。

Results: At the concentrations of 0.1, 1 and 10 μmol/L, berberine dose-dependently suppressed the formation of TRAP-positive multinucleated cells, the TRAP activity and the osteoclastic bone resorption. The strongest inhibitory effect was exhibited at the concentration of 10 μmol/L, with the inhibiting rate of 60.45%, 42.12% and 72.69% respectively.

结果：小檗碱在0.1~10μmol/L范围内，浓度依赖性地抑制TRAP阳性多核破骨细胞的形成和TRAP活性，减少破骨性骨吸收陷窝的面积；在10μmol/L浓度下，对破骨细胞的抑制作用最强，对TRAP阳性多核破骨细胞的形成和TRAP活性的抑制率分别达到了60.45%和42.12%，骨吸收陷窝面积减少72.69%。

Results: Myricetin and quercetin other than kaempferol showed the inhibitory effects against p110β PI3K, and their IC50 was 2.93μmol/L and 9.91μmol/L, respectively.

结果：杨梅素和榭槲皮素对重组人PI3K p110β催化亚基有抑制作用，IC50分别为2.93μmol/L和9.91μmol/L，而山萘黄素对PI3K p110β催化亚基则无抑制作用。

METHODS: Cultured THP-1 monocytes were induced to macrophages by 0.1 μmol/L phorbol myristate acetate, and the differentiated THP-1 macrophages were incubated with 200 μmol/L Hcy for 24, 48 and 72 hours respectively. Then cell supernatant and lysate were collected as condition medium.

在由0.1 μmol/L 佛波脂诱导分化的THP-1单核细胞中加入200 μmol/L 的高同型半胱氨酸培养24，48，72 h，将各时间点细胞上清液及裂解液作为条件培养基，加入经地塞米松及维生素D3作用6 d 后的培养瓶中继续培养24 h。

The contents of chlorophyll a in the water surface is between 7~21μg/l and the lower layer is under 5μg/l. The most dominant species of nannoplankton is Trachelomonas sp.

结果发现水库生态高度稳定，水质介於轻微到中度有机物污染，尚达不到优养化之地步，水表之叶绿素a的含量最多，但都在7~21μg/l之间，但中、下层很少不超过5μg/l。

The induced rate of rooting with naphthaleneacetic acid was higher than indolebutyric acid. The medium supplemented with 50 mg/l ascorbic acid and 0.5 mg/l NAA could improve the growth and development of root system. 57.5% of shoots could develop to the well-rooted plantlets and develop into healthy and fertile plants when planted in soil.

将芽体分割进行发根试验的结果显示，naphthaleneacetic acid诱导根系的效果优於indolebutyric acid，添加50 mg/l抗坏血酸和0.5 mg/l NAA之培养基则有更好的效果，约有57.5%的芽体可再生成具有旺盛根系的植株，经移植至土壤中也可生长健旺及正常开花。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。