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Full-thickness articular cartilage defects (diameter: 3mm, depth: 3mm) were made to bilateral condylus medialis femoris of all rabbits. In periosteum+BMSCs group and periosteum group, defects were covered by homolateral autogenous proximal tibia periosteums, with germinal layer facing to cavitas medullaris. After that, the periosteum+BMSCs group received 3 sutures, followed by injection of 20 μL BMSCs suspension (1×109/L) into the defects, after which the last suture was taken.

在全部兔双侧股骨内侧髁造成直径3 mm,深度3 mm的全层软骨缺损,骨膜复合骨髓间充质干细胞组、单纯骨膜组取同侧胫骨上段内侧骨膜,骨膜生发层朝向骨髓腔覆盖软骨缺损,骨膜复合骨髓间充质干细胞组先期缝合3针,向缺损区注入1×109 L-1骨髓间充质干细胞细胞悬液20 μL,植入细胞后缝合最后1针。

Methods: 100KunMing mice were divided into four groups randomly, each group included 25 mice inoculated EAC cells. The mice were treatedby celiac injection with saline solution , L-Arg , 5-Fu, L-Arg + 5-Fu respectively in each group . The natural survival time andthe average hydroperitoneum capacity were recorded for each group before the mice were killed.

将100只昆明小鼠随机分成4组,接种EAC细胞,依照分组向腹腔注射生理盐水、L-Arg、5-Fu、L-Arg+5-Fu,同时记录各组小鼠自然存活时间、处死时平均腹水量。

Respiratory-related burst activities were recorded from hypoglossal nerve rootlets before and during superfusion of theslice preparation with L-Arginine, sodium nitroprusside or 7-nitro indazole (7-NI, an in-hibitor of NO synthase).

同时保留舌下神经根,用改良Kreb's液灌流脑片并记录与之相连的舌下神经根呼吸节律性放电(respiratory rhmical dischargeactivity,RRDA),在灌流液中分别给予不同浓度的NO供体硝普钠(sodium nitroprusside,SNP),NO合成前体L-精氨酸(L-Arrginine,L-Arg)以及神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)特异性抑制剂7-nitro indazole(7-MI),观察其对RRDA的影响。

The relevant controlling measures are selection of proper bacterial species for lower higher alcohols formation; inoculative use level(1.3~1.5)×107/ml;α-aminonitrogen in malt liquid should be controlled 165~185mg/L, pH value 5.2~5.6; oxygen dissolution 8~10mg/L; the temperature controlled below 12℃; and proper fermentation degree.

控制方法主要有:选择高级醇生成量较低的菌种,接种量控制在(1.3~1.5)×10 7个/ml;麦汁中α-氨基氮控制在165~185mg/L,pH在5.2~5.6,溶解氧在8~10mg/L;主酵温度控制在12℃以下,控制适当的发酵度。

The results indicated that the complex was bonded to ct-DNA through a classic intercalative mode with an intrinsic binding constant of (5.97±0.27)×105 mol-1·L (50 mmol/L NaCl).

结果表明配合物以经典的插入模式与DNA键合,键合常数 K b=(5.97±0.27)×105 mol-1·L (50 mmol/L NaCl)。

When intermitted aeration was used, a dry cell weight of 2.62 g/L and an ethanol concentration of 28.9 g/L were achieved after 38 hrs of cultivation. Another fermentation was performed by feeding a 275 g/L glucose solution.

酦酵过程中,以间歇通气式批次酦酵培养(每通气3分钟停止通气10秒钟),经过38小时后,菌体乾重可达到2.62 g/L,酒精浓度为

Feeding of glucose was started at the end of the exponential phase of yeast growth. Intermitted aeration for 10 sec at every 3 min interval was used for the fed-batch fermentation. A dry cell weight of 8.22 g/L and an ethanol concentration of 73.7 g/L were achieved after 60 hrs of cultivation.

以葡萄糖浓度275 g/L进行批次馈料酦酵培养,当菌体生长至对数生长的末期时,通气方式改变为每间隔3分钟通气10秒钟,於60小时后菌体乾重可达到8.22 g/L,酒精浓度为

Results Median urinary iodine excretion was increased from 64.5 μg/L to 231.0 μg/L after salt iodization.

结果 食盐加碘后尿碘中位数由64.5 μg/L上升到231.0 μg/L。

The cytomorphological changes of K562 cells were observed in the electron microscope.Results RRTJ at the concentration of 1μL/mL expressed obviously inhibitory effect on the growth of K562 cells.The inhibitory effect at the concentration of 2μl/ml went up to its highest at 83.4%.RRTJ showed virulence to K562 cells.The karyon changed because of RRTJ attending.

结果 每mL培养液含1μL刺梨汁即对肿瘤细胞增殖有明显的抑制作用,每mL培养液含2μL刺梨汁抑制作用达到最大,为83.4%,再加大剂量抑制作用无明显升高,各种剂量的刺梨汁对细胞均无毒性,刺梨汁的加入使得细胞体积明显变小,细胞核形态发生改变,异染色质增多。

Results When the cells were treated with Sodium Selenite at a concentration of 2.0mmol//L lasting 72h, karyopyknosis and chromatin condensation were observed under microscope; When the cells were treated with Sodium Selenite at a concentration of 4.0mmol/L lasting 72h, the cells in G0/G1 and S phase was significantly decreased, a typical apoptosis peak before G1 phase was shown.

结果 2.0mmol/L亚硒酸钠作用72h, HE切片可见核固缩、染色质边集等改变;4mmol/L亚硒酸钠作用72h, G0/G1期和S期细胞明显减少,在细胞周期G1前期可见典型的细胞凋亡峰。

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L.A., L.A. (Kuwait Mix Marley Marl)
L-L-Lies
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L.O.U.D. L.O.U.D.
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L.I.L.Y.
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