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The capillary electrophoresis fingerprints of Fructus Forsythiae was established to control its quality. The background electrolyte was a 75 mmol/L sodium borate solution adjusted to pH 9.7 with 0.1 mmol/L NaOH solution. The detection wavelength was 228 nm and a voltage of 14 kV was applied. The Forsythia suspensa Thunb.

采用毛细管区带电泳法,以75 mmol/L硼砂溶液(用0.1 mmol/L氢氧化钠溶液调pH 9.7)为背景电解质,运行电压14 kV,检测波长228 nm,重力进样15 s(高度9 cm),建立了连翘药材的毛细管电泳指纹图谱。

The detection limits of potassium oxide, sodium oxide and zinc oxide were all less than 0.2 mg/L, and the background equivalent concentrations were all less than 6.0 mg/L.

方法测定氧化钾、氧化钠和氧化锌的检出限均小于0.2mg/L,背景等效浓度均小于6.0mg/L。

Effective length 40 cm was used. R un ning buffer was 2 mmol/L aqueous solution of sodium tetraborate containing 75 mm ol/L sodium dodecyl sulfate (pH 9.16) and 20% methanol.

毛细管柱,以75 mmol/L SDS-2 mmol/L 四硼酸钠溶液(外加体积分数为20%的甲醇)为缓冲液(pH 9.16)。

In optimum condition , the running buffer consisted of 40 mmol/L sodium tetraborate buffer(using 6mol/L hydrochloric acid adjustsd to 7.5),the UV detection wavelength was 210nm, separation voltage 20 kV , running temperation at 20℃.

考察了缓冲液离子浓度和pH,分离电压,运行温度等电泳参数,通过正交实验,确立了最佳电泳条件:缓冲液为40mmol/L硼砂,pH7.5(6mol/L HCL调节),紫外检测波长210nm,分离电压20kV,运行温度20℃。

On the media containing 0.02 and 0.04 g/L potassium sorbate, the contamination rates of explants were 87% and 97% resp. The bacteriostatic effect of 0.02 g/L potassium sorbate was obviously better than that of its other concn.

在含0.02和0.04 g/L山梨酸钾的培养基上外植体的污染率分别为87%和97%。0.02 g/L山梨酸钾的抑菌效果明显好于其他浓度。

It is shown from the study results that when the CDOM concentration ρ≥62.5mg/L, there was only a peak in the EEMS spectrograms, the excitation and emission wavelengths moved blue with decreasing CDOM concentration, and the move in wavelength and the change in CDOM concentration were well correlated; when ρ≤52.5mg/L, there were 3 peaks in the EEMS spectrograms, and the EEMS spectral shapes were basically invariant when the CDOM concentration changed.

结果表明:当CDOM质量浓度ρ≥62.5mg/L时,其EEMS均为单一峰,随着质量浓度降低,激发和发射波长向短波长方向移动,这种波长移动变化与质量浓度的变化呈良好的线性相关关系;当ρ≤52.5mg/L时,其三维荧光光谱显示3个峰。质量浓度在此范围内变化时,EEMS谱形基本不变。

When the CDOM concentration ρ≥150mg/L, the excitation and emission wavelengths in the SFS spectrograms moved blue with decreasing CDOM concentration, and the SFS spectral shapes also changed; when ρ50mg/L, the excitation and smission wavelegths in the SFS spectrograms didn't move, and the SFS spectral shapes were invariant, but the fluorescence intensity decreased with decreasing CDOM concentration.

不同质量浓度CDOM水溶液的SFS显示,当质量浓度ρ≥150mg/L时,随着质量浓度降低,激发和发射波长也向短波长方向移动,其谱形也发生变化;当ρ50mg/L时,激发和发射波长不发生漂移,谱形不变,荧光强度随质量浓度降低而降低。

METHODS: The serial subculture of HUVECs was used to investigate the effect of exogenous spermine with different concentrations (50 μmol/L-5 mmol/L) on HUVECs in different times (2 h, 4 h).

采用传代培养的方法,观察不同浓度精胺(50 μmol/L-5 mmol/L)对HUVECs作用2 h、4 h的影响。

"Kv ·= constant" principle, a sense of freedom laminated springs and valve switching power systems Plug Valves constitute △ P / S =εL S-feeling film work area,ε-spring Hook coefficient, L-valve stroke From this position of valve travel decisions every size of △ P value, if the valve travel position of the Kv and inversely proportional to the G = Kv · a constant value.

"Kv·=常数"原理,自由弹簧和感压膜构成阀门开关动力系统△P/S=εL S—感膜工作面积,ε—弹簧的胡克系数,L—阀行程由此可知阀门的每一个行程位置决定△P值的大小,如果阀行程位的Kv与成反比,则G=Kv·是恒定值。

The results indicated 2.0 mg/L of pp333 conduced to multiplication of squama,The best concentration for root growth was 0.5 mg/L.

结果表明:浓度为2.0 mg/L的多效唑有利于切花百合鳞片芽增殖;多效唑0.5mg/L为生根的最佳浓度

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