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The research results indicated that the best base was KOH, the best acid was H3PO4 and their neutralizing products were the necessary nutrients for foliar fertilizer; 12.4 L of foliar fertilizer were obtained in per kg of shrimp offal, chitin was obtained in 13.8% yield, crude protein was obtained in 23.2% yield using this method; This multifuctional foliar fertilizer was an abundant nutritional multifunctional foliar fertilizer which contained 15 kinds of amino acids, shimp cephalin, astacein and many trace elements. The concentration of calcium was 38.9 mg/L, the concentration of magnesium was 55.4 mg/L, the concentration of potassium was 20.0 g/L, the concentration of nitrogen was 4.5 g/L and the concentration of phosphorus was

研究结果表明：(1)处理虾下脚料的最佳碱是KOH，最佳酸是H3PO4，其中和产物是叶面肥必需的营养素；(2)本工艺不仅每Kg虾下脚料可制备虾叶面肥12.4L，而且可回收13.8％甲壳素和23.2％粗蛋白质；(3)经分析测定虾叶面肥中，含钙(Ca2+)38.9 mg/L，含镁(Mg2+)55.4 mg/L，含钾20.0 g/L，含氮4.5 g/L，含磷 78.9 g/L，还含有15种氨基酸及虾脑磷脂、虾红素及多种微量元素等，是一种营养丰富的多功能叶面肥。

By reseaching the tissue culture of Oxalis trisngularis, regeneration system of Oxalis trisngularis was preliminaryly established and the optimum mediums were MS+2,4-D 1.5mg/L+6-BA 0.1 mg/L for inducing callus and MS+NAA 0.9 mg/L +6-BA 0.4 mg/L for inducing differentiation, and MS+2,4-D 0.5mg/L for inducing rooting.

筛选出诱导愈伤的最佳培养基为MS+2，4-D 1.5mg/L+6-BA 0.1 mg/L；分化的最佳培养基为MS+NAA 0.9 mg/L +6-BA 0.4 mg/L；生根培养基为MS+2，4-D 0.5 mg/L；愈伤组织Kan抗性筛选的最佳浓度为58 mg/L。

Their structures were identified by chemical and spectral analysis. RESULTS Five compounds were isolated and identified as stigmasterol 3-O-β-D-glucopyranoside, 20β-hydroxy-ecdysone,β-L-thymidine, pennogenin 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside and pennogenin 3-O-α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)-]-β-D-glucopyranoiside.

结果 分离并鉴定了5个化合物，分别是Δ5，22-豆甾醇3-O-β-D-葡萄吡喃糖苷，20β-羟基蜕皮激素，β-L-脱氧胸腺嘧啶苷，偏诺皂苷元3-O-α-L-鼠李吡喃糖基－(1→2)-β-D-葡萄吡喃糖苷和偏诺皂苷元3-O-α-L-鼠李吡喃糖基－(1→4)-α-L-鼠李吡喃糖基－(1→4)-［α-L-鼠李吡喃糖基－(1→2)-］-β-D-葡萄吡喃糖苷。

Based on data collected at four stations in Daning River in the spring of 2004 and 2005, phytoplankton community structure, vertical distribution, abundance, biomass, C contents, diversity index, richness index and evenness index were studied. The causes of eutrophication and water blooms in Daning River were analysed and control measures were then put forward. The dominant species were Asterionella Formosa, Cryptomonas ovata, Cyclotella caspia, Stephanodiscus neoastraea in the spring of 2004 and those were Pandorina morum, Eudorina elegans, Cyclotella caspia, Cryptomonas ovata in the spring of 2005. Average cell densities, biomass, C contents in the spring of 2004 and 2005 were 171.1×10^5 cells/L, 12.2 mg/L, 1732.2g C/L and 113.1×10^5 cells/L, 10.1 mg/L, 1395.9g C/L, respectively.

报道了大宁河2004年春季和2005年春季浮游藻类的种类组成、垂直分布、数量、生物量以及C含量的调查结果，并应用多样性指数、丰富度指数和均匀性指数对其水质进行了综合评价；对大宁河蓄水后出现的富营养化现象和春季发生&水华&的原因进行了探讨，同时提出了控制水体富营养化的建议。2004年春季，藻类优势种为美丽星杆藻、卵形隐藻、里海小环藻和新星形冠盘藻；2005年春季的优势种为实球藻、空球藻、里海小环藻和卵形隐藻。2004年和2005年的细胞密度、生物量和C含量分别平均为171.1×10^5 cells/L，12.2 mg/L，1732.2g C/L和113.1×10^5 cells/L，10.1 mg/L，

4Mm harvested in preculture, is the best. In this form, Rhizopus oryzae R1021 produced most L-lactic acid (76. 62g/L), the pellet size in production medium in flask and fermentor are 1. 9mm and 2. 6mm, respectively, and its L-lactic acid productivity reached or exceeded that of the immobilized cell with polyurethane foam. The produced L-lactic acid and the yield of L-lactic acid based on the total glucose and the productivity are above 74g/L and 74% and 2. 69g/L h, respectively, in 7 batches repetitive fermentation in fermentor, and the size of pellets remain 2. 6mm～3. 4mm.

摇瓶种子培养获得的菌球直径为1.4mm时，这种大小的菌球产酸最高，为76.62g/L；研究表明以直径1.4mm的菌球为种子，在摇瓶发酵和发酵罐发酵中菌球大小分别为1.9mm和2.6mm；这种菌球的产酸水平最高，可以达到甚至超过聚氨酯泡沫载体固定化细胞发酵乳酸的水平；在反复7批次发酵罐发酵过程中，菌球的大小始终保持在2.6mm～3.4mm之间，L-乳酸产量、得率和生产能力分别保持在74g/L、74％和2.69g/L h以上。

The supernatant was removed. The wells were divided into five groups with 12 wells in each group. The control group was added with 1 g/L dimethyl sulphoxide and 3.3 mmol/L or 16.7 mmol/L glucose, 1 mL per well. The four experimental groups were added with 1 mL 0.2 μmol/L, 1 μmol/L, 5 μmol/L, or 10 μmol/L clozapine apart from the above DMSO and glucose.

每12孔为一组，共5组：对照组的孵育液含1 g/L二甲基亚砜、3.3 mmol/L或16.7 mmol/L的葡萄糖液1 mL；4个不同浓度氯氮平组的孵育液除含有上述成分外，还分别含浓度为0.2，1.0，5.0或10.0μmoL/L氯氮平；各组有6孔继续孵育1 h，另外6孔继续孵育4 h；吸取上清液，保存于-20℃冰箱中待测。

Results were shown as followings:(1) Sodium selenite at 0～2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5～2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下：（1）0～2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力；而对SMMC-7721细胞的作用不显著；（2）该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度；而对SMMC-7721细胞的作用均不显著；（3）高浓度硒（5μmol/L以上）显著性抑制L-02细胞生长、致细胞过氧化，灯盏花素能拮抗硒所致过氧化、降低硒毒性；（4）0.5～2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧，同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著；（5）富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白，其结构和功能有待研究；（6）通过已有的含hTERT基因质粒，成功构建hTERT反义RNA表达质粒，转染SMMC-7721细胞后对其生长具有抑制作用。

But the levels of 〓 elevation were lower than that in normal Tyrode's solution (P＜0.01 respectively), indicating that Ca〓 influx did contribute to the elevation of 〓. Ryanodine remarkably blocked the 〓 elevation by Str 1, 100nmol/L and 10μmol/L in normal Tyrode's solution, while ryanodine 10μmol/L, ruthenium red 10μmol/L and caffeine 10 mmol/L completely blocked the effects of Str on 〓 elevation in Ca〓- free Tyrode's solution (P＜0.01 respectively).

去除细胞外液中的钙离子，Str 1，100nmol/L和10μmol/L仍能分别升高〓到13±5％，71±8％和145±14％，但幅度低于其在正常台氏液中的作用。10μmol/L的ryanodine，10μmol/L钌红或10mmol/L咖啡因均可完全阻断Str 1，100nmol/L和10μmol/L在无钙台氏液中的作用(P＜0.01)。

Result The results indicated that the effects on Qixinghong of gibberellic acid 800 mg/L+spermidine 70 mg/L treatment was obvious, followed with 6benzyladenine 150 mg/L+naphthalene acetic acid 60 mg/L treatment.

结果］结果表明，GA 800 mg/L+Spd 70 mg/L处理七星红山茶的效果较好，BA 150 mg/L+NAA 60 mg/L次之；GA 500 mg/L+BA 120 mg/L处理红芙蓉山茶的效果较好，GA800 mg/L+NAA 80 mg/L对红芙蓉山茶有一定的促进作用。

Mmol/L to juvenile Japanese sea bass, and 75.86, 63.46, 56.42 mmol/L to black rockfish. When pH is steady at 8.40 + 0.10, the 24-, 48-, 96-hour LC50 of Alk are 72. 79, 65. 28, 57. 92 mmol/L to Japanese sea bass, and 69. 47, 59. 37, 51. 96mmol/L to black rockfish. It is obvious that the toxicity of Alk to fish increases with the progressive increase of Alk or time when pH is at a constant level.3. According to analysis of variance of orthogonal test, pH and Alk have signification effects on the mortality rate of black rockfish.

pH在正常养殖范围时，通过碱度对海水鱼类鲈鱼、黑鲪的急性毒性试验，得到pH=8.10±0.10时，碱度对鲈鱼稚鱼(体长1.6～2.2cm)24、48、96h的半致死值为60.88、44.36、36.06mmol／L，安全浓度为7.07mmol／L；pH=8.05±0.10时，碱度对黑鲳幼鱼24、48、96h的半致死值为75.86、63.46、56.42mmol／L，安全浓度为13.32mmol／L.pH=8.40±0.10时，碱度对鲈鱼幼鱼24、48、96小时的半致死值为72.79、65.28、57.92mmol／L，安全浓度为15.76mmol／L；对黑鲪幼鱼24、48、96h的半致死值为69.47、59.37、51.96mmol／L，安全浓度13.01mmol／L。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失