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The results indicated that the best induce medium for bud propagation was B5 + 0.1 mg/L NAA + 3 mg/L 6-BA. The numbers of hormone and nutritious and the period cultured were different in different varieties. The garlic shoot-tip with some bud scale is better for culturing. It can increased the survival rate of virus eliminating seedling and propagate rate.

结果表明，最佳诱导培养基为B5+0.1mg/L NAA+3mg/L 6-BA，但是，激素、营养物质的添加数量以及培养时期不同品种间有差异；剥离大蒜茎尖带一定数量的鳞片为好，其不仅可提高脱毒苗成活率，而且有利于扩大茎尖繁殖系数。

The results indicated that the best induce medium for bud propagation was B-5+0.1 mg/L NAA+3 mg/L 6-BA.The numbers of hormone and nutritious and the period cultured were different in different varieties.The garlic shoot-tip with some bud scale is better for culturing.

结果表明，最佳诱导培养基为B5+0.1 mg/L NAA+3 mg/L 6BA，但是，激素、营养物质的添加数量以及培养时期不同品种间有差异；剥离大蒜茎尖带一定数量的鳞片为好，其不仅可提高脱毒苗成活率，而且有利于扩大茎尖繁殖系数。

Result The scale leaf formation was inhibited by MeJA, the higher the concentration of MeJA the more efficient for restraining scale leaf growth. 100.0% of bulblet formation was obtained in the treatment of 1.0 μmol/L MeJA, but the scale leaf growth was significantly lower than control. 3.0 μmol/L MeJA had the inhibitory effect to both the bulblets and the scale leaves.

结果］茉莉酸甲酯对"Mona"试管百合鳞片叶生长有抑制作用，浓度越高其效果越明显；当茉莉酸甲酯浓度为1.0μmol/L时，鳞片叶发生率(87.9%)明显低于对照(100.0%)，但鳞茎分化和生长也显著好于对照；但茉莉酸甲酯浓度超过3.0μmol/L时，不仅对鳞片叶而且对鳞茎生长也有抑制效果。

Take for the amount of test materials, precision that set, place beaker, unless otherwise specified, may be added 40ml of water and hydrochloric acid solution (1-2) 15ml, and then set on a magnetic stirrer, stirring allows the dissolved, together with potassium bromide 2g insert platinum - platinum electrode, it will insert the solution of the following buret tip is about 2 / 3, using sodium nitrite titration solution (0.1mol / L, or 0.05mol / L) rapid titration, side drops while stirring, to near point, Burette tip surface will be presented with a small amount of water leaching cutting-edge, lotion, and solution and continue titration slowly until the sudden deflection of galvanometer pointer, namely, titration end point.

取供试品适量，精密称定，放置烧杯中，除另有规定外，可加水40ml与盐酸溶液（1-2）15ml，而后置电磁搅拌器上，搅拌使溶解，再加溴化钾2g，插入铂-铂电极后，将滴定管的尖端插入溶液下面约2/3处，用亚硝酸钠滴定液（0.1mol/L或0.05mol/L）迅速滴定，边滴边搅拌，至近点时，将滴定管的尖端提出液面，用少量水淋洗尖端，洗液并溶液中，继续缓缓滴定，至电流计指针突然偏转，即为滴定终点。

The effect of 2 concentration (40 g/L and 80 g/L) of the cooked water of canna, coconut, betel nut and narcissus ball added in potato medium on the mycelial growth of Pleurotus geesteranus was investigated, respectively.

方法］探讨马铃薯综合培养基中分别加入40g/L和80g/L 2种浓度的美人蕉、大王椰子、假槟榔和水仙球废材的水煮液对秀珍菇菌丝生长的影响。

The results showed with the increasing of NaCl concentration, the phenomenon of carnification could be found in the leaves of Arabidopsis thaliana . Under the stress of NaCl lower than 100 mmol/L, the tissue structure of root had no distinct difference from the control. When the concentration of NaCl was higher than 250 mmol/L, the stained color of safranine was deep but not even, and color in fibrovascular tissue and peripheral cortex cell were deeper than that around fibrovascular tissue.

当NaCl浓度小于100 mmol／L时，根组织结构与对照未表现出明显的差异；但当NaCl浓度达到250 mmol／L时，根组织结构对番红染料的着色较深而且不均匀，维管组织和外围皮层细胞着色较深，而维管组织周围的细胞着色较浅。

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DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA，目的DNA的聚合酶链反应扩增及电泳检测，5-羟色胺2A受体基因T102C基因片断扩增的引物序列：5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3'；采用50 μL反应体系，DNA 0.5 μg，引物50 pmol，TagDNA酶2U加dNTP至终浓度200μmol/L。

Corn stalk pretreated by dilute sulfuric acid was hydrolyzed by the dosages of 20 FPIU/g Trichoderma reesei cellulase and 4 IU/g β-glucosidase at the substrate concentration of 100 g/L for 48 h, the concentration of cellobiose and glucose in hydrolysate were 1.12 and 42.68 g/L, respectively, the yield of enzymatic hydrolysis and the ratio of fermentable sugar were 62.85% and 97.44% respectively.

底物质量浓度为100g/L的稀硫酸预处理玉米秸秆，经酶用量为20 FPIU/g的里氏木霉纤维素酶和4IU/g的β-葡萄糖苷酶水解48h，水解糖液中纤维二糖和葡萄糖质量浓度分别为1.12和42.68g/L，纤维素水解得率和可发酵性糖的比例分别为62.85%和97.44%。

An aerosol spray device which is capable of imparting a unipolar charge by double layer charging and charge separation to liquid droplets of a composition sprayed therefrom has a spraying head in the form of an insert in an actuator, the spraying head having a bore through which liquid is expelled having an outlet, preferably with a tortuous periphery, having an L/a ratio of at least 8 (preferably at least 10) where L is the length of the periphery defining the bore outlet in mm and a is the cross-sectional area of the bore outlet in mm2 and the apparatus being constructed such that the droplets are expelled from the spraying head at a flow ratio of at least 0.4 (preferably at least 0.5) grams per second and have a charge to mass ratio of at least +/-1x10-4 C/kg.

本发明还提供了一种喷射装置，它能够通过双层充电和电荷分离而向要喷出的组合物液滴施加单一极性的电荷，该喷射装置具有一个喷头，该喷头制成为致动器中的插入件的形式，喷头具有一个孔腔，液体能够通过该孔腔从装置中排出，该孔腔具有一出口，该出口最好具有曲线周边，并且L/a比率至少为8，其中L是以mm为单位的确定孔腔出口的周长，a是以mm2为单位的孔腔出口的横截面积，该装置构造成使得液滴在至少每秒0.5克的流动速率以及具有至少＋／－1×100C/kg的电荷／质量比的状态下从喷头排出。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。