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Results were as follows: in MS basic medium+KT2mg/L or 2ip, the two varieties growed well. Appending NAA0.2 mg/L could promote radication commonly. But in MS+BAP2 mg/L there was obvious difference from the two varieties. Chinese yam rhizome axillary bud was restrainted. But feng yam was prone to form fascicular bud. When supplemented with NAA0.2 mg.L-1, NAA and BAP could accelerate Chinese yam rhizome axillary bud growth and increase the number of buds.

结果表明:两品种在MS+KT2mg/L或2ip培养基上生长情况均较好,当附加NAA0.2时,一般可促进生根;但在培养基上,两品种差异明显,淮山药表现腋芽生长受抑制,而凤山药则易形成丛生芽,而当此培养基附加NAA0.2时,NAA与BAP可协同作用促进淮山药的腋芽生长,增加芽苗数,但对凤山药的生长则基本无影响。

Being as a NO donator,L-Arg siguitlcanfly enhanced the ability of TNF-α which increasing permeability of strial capillary cndothelial cells;permeation rate to Evans blue beightened significantly(P<0.01),which obviously rose up when the concentration of Evans blue added.L-NMMA,NO inhibitor,was able to weaken the ability of TNF-α which increasing permeability of strial capillary endothelial cells:permeation rate to Evans blue decreased siguificantly (P<0.01),which obviously fell down when the concentration of L-NMMA added.

一氧化氮(nitric oxide,NO)供体L-Arg能增加TNF-α作用下耳蜗血管纹微血管内皮细胞的通透性,使伊文思蓝的通透率升高,差异具有统计学意义(P值均<0.01),且随着L-Arg浓度的增加内皮细胞对伊文思蓝的通透率也明显增加。

Using Eriochome cyanine R staining, we found that Al accumulation on surface of root tips was positive related to the inhibition of root growth in treatments of 0 - 80μmol·L~(-1) Al for 24 h. Exchangeable Al in apoplast and symplasm of root tips of wheat was effectively desorbed by 5 mmol·L~(-1) CaCl_2 at 4℃.

铬化青R染色可以原位显示0-80μmol·L~(-1)Al处理后根尖表面Al的吸附量与根尖伸长受抑制程度具有很好的一致性。5mmol·L~(-1) CaCl_2溶液可以有效洗脱10和40μmol·L~(-1)Al处理24小时后小麦根尖质外体和共质体中可交换态Al。

On the normotensive rat, bilateral microinjection of L-arginine (16 n mol/0.1 ul/site), a precursor of nitric oxide into the rVLM, caused a decrease in BP, HR and the pressor response induced by the dPAG stimulation, while microinjection of NG-methyl-Larginine (L-NMMA, 16 n mol/0.1 ul/site), a blockade for NO to form, into the rVLM bilaterally, caused an increase in BP, HR and the pressor response induced by the dPAG stimulation. There was no effect of both drugs mentioned above on the tachycardiac response induced by the dPAG stimulation.

五、在正常血压大鼠,于双侧rVLM微量注射一氧化氮前体(16nmol/0.1μl/site)可引起BP降低、HR减慢、以及电刺激dPAG诱发的防御性升压反应减弱;而于同区微量注射生成一氧化氮的拮抗剂N〓-甲基-L-精氨酸(16nmol/0.1μl/site)则可引起BP升高、HR加快、以及电刺激dPAG诱发的防御性升压反应增强;而上述两药对电刺激dPAG诱发的防御性加快心率反应均无明显作用。

Results Compared with solvent control,toxaphene increased significantly MN frequencies in Hep G2 cells at concentrations of 20,40?μmol/L respectively(P.01,P.01).No significant increase of MN frequencies were found in Hep G2 cells treated with Aroclor1254(23~184?μmol/L) and DDT17.8~60?

结果20,40μmol/L毒杀芬处理Hep G2细胞的微核率与溶剂对照相比显著增加(P.01,P.01);经Aroclor1254(23~184μmol/L)和滴滴涕(17.8~60μmol/L)处理的Hep G2细胞的微核率与溶剂对照相比,差异无统计学意义。

The effect of humic acid with the concentration of 850mg/L on improving the drought-resistance of Populus alba x Populus breolinensis seedlings was very obvious, and favorable regulative effects of humic acid with the concentrations of 850 and 650 mg/L on Ulmus pumila seedlings were also observed, but the effect of humic acid on Betula platyphylla seedlings was not distinct.

浓度为850 mg/L的黄腐酸对提高银中杨苗木抗旱性效果明显,浓度为850 mg/L和650mg/L的黄腐酸对白榆苗木抗旱性均有良好的调节作用,黄腐酸对白桦苗木抗旱性的调节作用不明显。

Results AE from CM had vasodilating effect on the contracti on of mesentery blood capillary in guinea pigs and the contraction of rat thoracic aortic ring induced by l0μmol/L phenylephrine, even under the condition of pre-treating with glibenclamide (1μmol/L) or propranolol (3μmol/L). When the endothelium of the rat thoracic aortic ring was removed, AE from CM had not vasodilating effect hut constrictive effect. AE from CM could also increase the contents of NO, NOS and cNOS in the aortic tissue and had no obvious effect on the NO content in plasma and iNOS in aortic tissue of rats.

结果 桑白皮丙酮提取物有抑制去甲肾上腺素收缩豚鼠肠系膜微血管的作用,抑制苯肾上腺素(10μmol/L)收缩离体大鼠胸主动脉环的作用,预加优降糖或普蔡洛尔(3μmol/L)后仍然有松弛作用;大鼠胸主动脉环去内皮后药物无松弛作用,反而起收缩作用;显著升高血管的NO和NOS、cNOS含量,对大鼠血浆的NO和血管iNOS的含量影响不大。

The results were very successful.The removal rates of chromaticity and CODCr reached 96.56% and 94.46% respectively under the optimal conditions as follows:dosages of H2O2 and Fe2+ were 4.0g/L and 0.3 g/L respectively,microwave irradiation power was 500 W,and radiation time was 1 min.The investigation shows that the microwave irradiation,homogeneous Fenton oxid...

结果表明,微波-Fenton氧化-PASS混凝法适合于处理复杂垃圾渗滤液,在H2O2的质量浓度为4.0 g/L,Fe2+质量浓度为0.25 g/L,pH值为3.0,固定微波辐射功率500 W,辐照1 min,PASS质量浓度为25 mg/L的最佳条件下,出水色度仅15倍,脱色率和CODcr去除率分别达到96.86%和94.46%;微波辐射、Fenton氧化和PASS混凝沉淀结合处理垃圾渗滤液废水时具有较好的协同效应。

Methods The spontaneous contraction of gastric smooth muscle strip was recorded by using physiograph in rats. The change of tension in response to different concentrations of CNP and sodium nitroprusside, co-incubated with high concentration glucose (30 mmol/L) or mannitol were detected.

取大鼠40只制备胃窦环形肌条,肌条收缩活动稳定后,对照1、2组分别加入不同浓度CNP1×10^(-8、3×10^(-8)、1×10^(-7)mol/L及硝普钠1×10^(-7、3×10^(-7)、1×10^(-6)mol/L,观察其效应,每次加药观察20 min,冲洗3次;高糖1、2组预加葡萄糖使浴槽终浓度为30 mmol/L,其后操作同对照1、2组;甘露醇组预加甘露醇30mmol/L,其后操作同对照1组;均采用多道生理信号采集处理系统计算CNP致平滑肌张力的抑制率。

The ISSR fingerprinting of 18 Japanese plum cultivars and 12 plum species or variety were established by using optimum system with primer 5"-9T-3"and 5"-9C-3", respectively. The identification rate of plum cultivars and species or variety was 100%. The effects of five important components on reaction of SSR were studied with Prunus salicina cv. Meilili. The results showed that the optimum concentration of five important components i.e. Tag DNA polymerase, Mg +, single primer, template DNA and dNTPs in 25 μL reaction system of SSR were 1.5u, 2.0mM, 0.8 μ M, 30~40ng and 0.16~0.24mM, respectively. The dendrograms of these cultivars was contructed according the Jaccard coefficient of similarity.

利用该优化体系,以5′-_9T-3′为引物,构建了中国李18个品种的ISSR指纹图谱,该引物可将这些品种完全区别开来;以5′-_9C-3′为引物,构建了李属6类种质资源的ISSR指纹图谱,该引物区分率为100%。4、中国李品种适宜的SSR反应分析体系是:25μL SSR反应体系中,Taq DNA聚合酶、Mg~(2+)、每个引物、模板DNA和dNTPs等5种成分的适宜浓度分别是:1.5u、2.0mmol·L~(-1)、0.8μmol·L~(-1)、30~40ng和0.16~0.24mmol·L~(-1)。5、采用RAPD、ISSR和SSR等三种分子标记技术对中国李品种资源54份和2个欧洲李品种进行了遗传多样性分析,并以品种间的遗传相似系数构建了Jaccard聚类树状图。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。