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Results The IC50 of SPG-Rg3 was (155.7±0.71) mg·L-1,when the concentration of SPG-Rg3 was arranged from 37.5 to 600.0 mg·L-1,the growth inhibitory rate of MCF-7 cells was higher following the increase of its concentration,the cell growth was greatly inhibited compared with control group(P.05); cell cycle was changed,the cell number of S period increased compared with control group(P.01),the cell number of G2/M period decreased compared with control group(P.01),in experiment group there was an apoptotic apex before G1 apex,and the number of apoptotic cells increased; most of cell nuleus in SPG-Rg3 (150 mg·L-1)experiment group appeared yellow or chrysoidine fluorescence,contracted,beaded and crescent; the result of immunocellularchemical staining of caspase-8 indicated that caspase-8 protein had no expression in control group,but stronger expression in SPG-Rg3 group.

结果:SPG-Rg3 IC50为(155.70±0.71) mg·L-1,当SPG-Rg3浓度在37.5~600.0mg·L-1时,MCF-7细胞的生长抑制率随SPG-Rg3浓度的增加而增大,与对照组比较,细胞增殖受到明显抑制(P.05);MCF-7细胞的生长周期也发生变化,S期细胞数增加,明显高于对照组(P.01,G2/M期细胞数则明显少于对照组(P.01;并且在G1峰前出现明显的凋亡峰,凋亡细胞数增加。

Both of amount of 54 ind/L and 95 ind/L were found from cladocera of 8 species as well as Copepoda of 6 species and their proportion of 28.57% and 21.43% were recorded.

浮游动物28个种属,其中轮虫14个种属,数量137 ind/L,占50%;枝角类8个种属,数量54 ind/L,占28.57%;桡足类6个种属,数量95 ind/L,占21.43%。

When the embryogenic calli were transferred to medium MS+5mg/L ABA+50g/L PEG+5mg/L AgNO〓 for one month, a lot of normal cotyledonary somatic embryos were produced.

当胚性愈伤组织转到MS+5mg/LABA+50g/L PEG+5mg/L AgNO〓的分化培养基上,1个月后可产生大量正常的子叶期成熟体细胞胚。

Neurons treated with 20-mmol/L-theophylline did not show 180bp ladder characteristic of endonuclear activation on its DNA gel electrophoresis. The [cAMP]i of the neurons rose after being incubated together with different concentration theophylline (5-80 mmol/L) for 15 minutes in CO2 incubator and the prometed range relied on the dose of theophylline.

100%,量效关系有高度显著性(P.01),EC50=2.97mmol/L;经茶碱(20mmol/L)处理的细胞,其DNA凝胶电泳带没有&梯子&状分布;与不同浓度茶碱(5-80 mmol/L)在CO2培养箱孵育15 min,小脑颗粒神经元内cAMP均升高,且是剂量依赖性的。

L-FABP gene may be involved in the carcinogenesis of human HCC. Expression of L-FABP is associated with VEGF expression, suggesting that L-FABP promotes the growth of blood vessels by taking up the fatly acids from the bloodstream, and both of them produce a marked effect on energy metabolism in HCC.

L-FABP、VEGF基因以及蛋白质在肝癌组织中表达均显著上调,并且二者具有正相关性、我们推测L-FABP在获取更多的脂肪酸的同时可以促进血管的增生,进而促进了肝癌的发展。

Further, at concentrationssimilar to the maximum therapeutic plasma concentrations (10–7 mol/L olprinone; 10–6 mol/L milrinone; 10–5 mol/Lamrinone) olprinone and milrinone were more potent at inducingrelaxations than amrinone in gastroepiploic arteries and radialarteries, whereas in internal mammary arteries milrinone wasmore potent than the others.

此外,在与最大治疗血浆浓度相近似的浓度中(10–7 mol/L 的奥普力农、10–6 mol/L 的米力农、10–5 mol/L 的氨力农)时,奥普力农和米力农对胃网膜和挠动脉的舒张作用比氨力农强;而对乳内动脉,米力农的舒张作用比其它两者更强。

The germinative number of horsebeans and peas were raised by 44% with activated water by tourmaline. 200μl 1 X 10~6/ml mouse's H_(22) cell, 200μ 4X 10~6/ml splenic cell, 200μl 1 × 10~6/ml epidermal cell were all inhibited after the action of tourmaline. It is analyzed that the factors which effected the multiplication of the cells included direct action which are tourmaline's electric field, far-infrared, negative ions and solvent ions, and indirect effect which are small water clusters and pH value.

电气石活化水使蚕豆和绿豌豆种子发芽速度均提高44%。0.5g电气石分别对200μl 1×10~6/ml的小白鼠H_(22)肝癌细胞,对200μl 4×10~6/ml小白鼠脾细胞,对200μl 1×10~6/ml的小白鼠表皮细胞的增殖起抑制作用,分析认为,电气石影响细胞增殖的因素包括电场、红外线、负离子和溶出离子对细胞的直接作用和通过改变水分子结构、培养基pH值的间接作用。

With the concentration of 0.1mol·L~(-1), sodium benzoate andβ-sodium glycerophosphate show obvious inhibiting effect, respectively. And D-sodium gluconate with the concentration ranged from 0.001 to 0.1mol·L~(-1) has excellent inhibiting effect.

结果表明,缓蚀剂的浓度为0.1mol·L~(-1)时,苯甲酸钠和β—甘油磷酸钠有较好缓蚀效果,D—葡萄糖酸钠浓度为0.001mol·L~(-1)至0.1mol·L~(-1)时均有良好缓蚀效果。

The passage 3 rat bone marrow mesenchymal stem cells were cultured at the density of 5×107/L in different groups, the induced group was added into conditioned medium containing 1×10-8 mol/L dexamethasone, 10 mmol/L beta-sodium glycerophosphate and 50 mg/L ascorbic acid at the same time of seeding, at 50%-60% confluence, at 85%-90% confluence, respectively.

将第3代大鼠骨髓间充质干细胞以5×107 L-1密度分组培养,诱导组分别于接种当时、细胞达50%~60%融合时、细胞达85%~95%融合时加入含地塞米松、β-甘油磷酸钠、维生素C的成骨诱导培养基诱导。

Both direct synthesis and ring-opening polymerization can be utilized to prepare homo- and copolymers of lactic acid such as L-lactide, D-lactide, D, L-lactide, and glycolide.

直接聚合和开环聚合两种方法可以用来制备乳酸的均聚物和共聚物,如聚L乳酸、聚D乳酸、聚D,L乳酸和L乳酸乙醇酸共聚物。

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