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In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

On the basic that existing second-order sufficient condition for an isolated minimizer of order 2 for unconstrained multi-objective optimization, using the so called oriented distance from a point to a set, l-stable function and generalized second-order Peano direction derivative, some second-order sufficient conditions for an isolated minimizer of order 2 for constrained multiobjective optimization problems with l-stable functions are obtained.

在已有的无约束l-稳定函数多目标优化问题的二阶充分条件的基础上，借助定向距离函数和l-稳定函数的性质及引入的广义二阶Peano方向导数，进一步刻画了具有抽象约束的l-稳定函数的多目标优化问题的二阶孤立极小点的二阶充分条件。

RESULTS: The total GKB yields were enhanced 69%, 13.8%and 11.4%compared with the control by adding 100 mg.L-1 of isoprene and low concentrations (10 and 50 mg.L-1) of geraniol in the medium, respectively.

结果：于培养基中添加100 mg.L-1异戊二烯及低浓度(10 mg.L-1，50 mg.L-1)的牛儿醇能提高GKB的产量，分别比对照增加了69%，13.8%和11.4%。

The results indicated that the inhibited concentrations of kanamycin of adventitious bud formation of Helianthus annus were 75 mg/L and 100 mg/L, when the carbenicillin was used in the transformation procedure; the concentrations was 300 mg/L when the growth of agrobacterium could be effectively inhibited. At the same time, the induction and growth of adventitious bud were not remarkably injured.

在以卡那霉素为抗性选择标记时，对油葵不定芽起完全抑制作用的Km临界浓度在75~100 mg/L；在以羧节青霉素为抑菌剂用于油葵遗传转化试验时，其浓度为300 mg/L时即可有效抑制农杆菌的生长，同时又不会对不定芽的诱导及生长产生明显的伤害作用。

Ethanol production by fed-batch fermentation was investigated and the maximum ethanol concentration of 94.81 g/L, equivalent to 81.54% of its theoretical value, was reached with final Jerusalem artichoke flour of 300 g/L in a 5 L jar fermenter.

在此基础上，对菊芋粉补料发酵进行了试验，补料至菊芋粉终浓度为300 g/L，发酵终点乙醇浓度为94.81 g/L，理论转化率为81.54%。

Next, we give the definition of Killing form hold for pointed YD-Lie algebra, let L be a pointed YD-Lie algebra, for every homogeneous elements x, y ∈ L, if = tr_q.then we call Killing form of L.

接着，给出了点YD-李代数的Killing型的定义：L为任意的点YD-李代数，如果齐次元x，y∈L定义：=tr_q。

The contents of chlorophyll, Vc, nitrate and oxalate of spinach which treated by 30 mg/L Arctium lappa L. extract were optimal. The fresh weight of spinach which treated by 500 mg/L Arctium lappa L.

其中，对叶绿素含量、Vc含量、硝酸盐及草酸含量的效应，都以30 mg/L处理的效果最好。

Based on the outcome of Xu Yang and Qin Keyun about lattice implication algebra and lattice-valued prepositional logic LP with truth-value in a lattice implication algebra, the author studied the properties of lattice implication algebra and the α-automated reasoning method based on α-resolution principle of LP. The specific contents are as follows: The Study of Lattice Implication Algebra On the basis of previous results of lattice implication algebra, this part consists of the following three points: 1. Some properties of lattice implication algebra L were discussed, and some important results were given if L was a complete lattice implication algebra. 2. The properties of left idempotent elements of lattice implication algebras were discussed, and the conclusion that lattice implication algebra L was equals of the directed sum of the range and dual kernel of a left map constructed by a left idempotent element was proved. 3. The properties of the filters of lattice implication algebra were discussed, the theorem was shown that they satisfy the hypothetical syllogism and substitute theorem of the propositional logic. 4. The concept of weak niters of lattice implication algebras and their properties and structures are discussed. It is proved that all weak filters of a lattice implication algebra form a topology and the the implication isomorphism betweem two lattice implication algebras is a topological mapping between their topological spaces. The Study of α-automated reasoning method based on the lattice-valued propositional logic LP In this part, the author given an a-automated reasoning method based on the lattice-valued propositional logic LP.

本文基于徐扬和秦克云的关于格蕴涵代数和以格蕴涵代数为真值域的格值命题逻辑系统LP的研究工作，对格蕴涵代数以及格值命题逻辑系统LP中基于α-归结原理的自动推理方法进行了系统深入的研究，主要有以下两方面的研究成果：一、关于格蕴涵代数的研究 1、对格蕴涵代数的格论性质进行了研究，得到了当L为完备格蕴涵代数时，关于∨，∧，→运算的一些结果； 2、对格蕴涵代数的左幂等元进行了研究，证明了格蕴涵代数L可以分解为任何一个左幂等元所对应的左映射的像集合与其对偶核的直和； 3、对格蕴涵代数的滤子的性质进行了研究，证明了滤子的结构相似于逻辑学中的Hypothetical syllogism规则和替换定理； 4、给出了格蕴涵代数中弱滤子的概念，对弱滤子的性质个结构进行了研究，证明了格蕴涵代数的全体弱滤子构成一个拓扑结构，格蕴涵代数之间的蕴涵同构是相应的拓扑空间之间的拓扑映射。

Moreover, the catalytic current presents linear dependence on the concentration of glucose in the range from 5μmol·L~(-1) to 2.8 mmol·L~(-1), with a detection limit of 1.0μmol·L~(-1) by amperometry.

安培法测得肼的催化氧化峰电流与肼的浓度在2.5μmol·L~(-1)-0.2 mmol·L~(-1)范围内呈线性关系。

The results showed that when the catalyst amount was 4 g/L,the molar ratio of L-ascorbic acid to malic acid was 1:3,and the reaction temperature was 55 ℃,the highest L-ascorbyl malate yield of 69.30% could be obtained.

结果表明：添加固定化酶量4g/L，底物配位物质的量比1：3，温度55℃，L-抗坏血酸苹果酸酯的得率最高为69.30%。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。