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Aldrin at concentrations higher than 40 μg/L decreased life expectancy at birth of F1 generation of the cladoceran, that at 20 μg/L and 80-640 μg/L decreased net reproduction rate, that at 320 and 640 μg/L shortened generation time, and that at all the concentrations decreased intrinsic rate of population increase. Aldrin at 160-640 μg/L shortened life expectancy at birth and generation time of F2 generation of the cladoceran, that at 160 and 640 μg/L decreased net reproduction rate, but that at all the concentrations except for 40 and 320 μg/L increased intrinsic rate of population increase.

浓度高于40μg/L的艾氏剂使多刺裸腹溞F1代的生命期望显著缩短，20μg/L和80~640μg/L的艾试剂使F1代的净生殖率显著降低，320μg/L和640μg/L的艾氏剂使F1代的世代时间显著缩短，所有浓度的艾试剂均使F1代的种群内禀增长率显著降低。160~640μg/L的艾氏剂使多刺裸腹溞F2代的生命期望和世代时间显著缩短，160μg/L和640μg/L的艾氏剂使F2代的净生殖率显著降低；除40和320μg/L外，所有浓度的艾试剂均显著提高了F2代的种群内禀增长率。

The questing for long service electroless Nickel technology leads to the preferable formula and operation conditions as follows: Nickel Sulphate 25g/L, Sodium Hypophosphate 30g/L, Lactic Acid 20~ 25ml/L, Citric Acid 5g/L, Sodium Acetate 15g/L, Thiourea l~3mg/L, Stabilizer E Img/L.

研究得出的最佳长寿命镀镍工艺为；硫酸镍25g/L，次亚磷酸钠30g/L，乳酸20ml/L，柠檬酸5g/L，乙酸钠15g/L，硫脲1～3mg/L，稳定剂E1mg／L镀液pH值5.0～5.1，施镀温度90±2℃，装载量1dm~2/L；每60min测量镀液中消耗的有效成分和补加浓缩液。

As a whole,the notable characteristic of primary productivity in size-fractioned structure was that nanoplankton occupied comparatively significant advantage in Beibu Gulf.Nanoplankton has the largest contribution to gross primary productivity,and picoplankton was the secondary contributor,while microplankton the least.The contribution of microplankton for primary productivity in the north Gulf was more than that in the other waters.Nanoplankton and picoplankton contribute more to the gross primary productivity in offshore deep waters than in inshore shallow waters3.The Beibu Gulf can be divided into three ecoregions:Region-Ⅰis the ecoregion in inshore shallow waters of the north Gulf.In average,the water depth is 18m,DIN is 1.88μmol/L,DIP is 0.20μmol/L,N:P is 9.4:1,dissolved silicate is 5.17μmol/L,the Chl a conentration in surface layer is 2.27mg·m~(-3),the assimilation index in surface layer is 3.80mg/,the primary productivity is 198.78mgC/(m~2·d), and potential fishery production is estimated to be 0.24gC/(m~2·a) according to the primary productivity.The ecoregion is mainly affected by the northern coastal water systems,and may be fit for aquaculture;Region-Ⅱis the ecoregion in offshore deep waters of the north Gulf and the coastal shallow waters to the west Hainan Island.In average,the water depth is 35m,DIN is 2.01μmol/L,DIP is 0.18μmol/L,N:P is 11.2:1,disovled silicate is 4.23μmol/L,the chlorophyll a of surface layer concentration is 1.45mg·m~(-3),the assimilation index of surface layer is 4.12 mg/,the primary productivity is 276.60mgC/(m~2·d),and the estimated potential fishery production is 0.34gC/(m~2·a) according to the primary productivity. The ecoregion was mainly influenced by ocean current from the South China Sea, rivers in the west coast of Hainan Island and the water from Qiongzhou Strait.It may be fit for aquaculture and fishery;Region-Ⅲis the ecoregion in offshore deep waters of the mid and south Gulf.In average,the water depth is 75m,DIN is 0.77μmol/L, DIP is 0.15μmol/L,N:P is 5.1:1,disovled silicate is 3.05μmol/L,the chlorophyll a of surface layer concentration is 0.70mg·m~(-3),the assimilation index of surface layer averaged is 3.69mg/,the primary productivity is 350.89mgC/(m~2·d),and the estimated potential fishery production is 0.43gC/(m~2·a) according to the primary productivity.The ecoregion was mainly affected by the circulation inside Beibu Gulf, and may be fit for fishery.

初级生产力的粒级结构的一个显著特点是总体上微型浮游生物在全调查海区均占较明显优势，对总初级生产力的平均贡献最大；微微型浮游生物对初级生产力的平均贡献次之；小型浮游生物对总初级生产力的平均贡献最小；湾北部小型浮游生物对初级生产力的平均贡献高于湾中部和湾南部，而湾中部和湾南部微型和微微型浮游生物对初级生产力的平均贡献高于湾北部，远岸深水区高于近岸浅水区。3、北部湾可以分为三个生态区：湾北部近岸浅水区，该区的环境主要特点是平均水深19m，DIN浓度平均值为1.88μmol/L，DIP浓度平均值为0.20μmol/L，N：P为9.4：1，硅酸盐浓度平均值为5.17μmol/L，表层叶绿素a平均值高达2.27 mg·m~(-3)，表层同化指数平均为3.80 mg/，初级生产力平均值198.78mgC/(m~2·d)，根据初级生产力计算潜在渔业资源碳生产量平均为0.24 gC/(m~2·a)，该区主要受湾北部沿岸水系影响，适合作为水产养殖区；湾北部深水区和海南岛西部沿岸浅水区，该区的环境主要特点是平均水深35m，DIN浓度平均值为2.01μmol/L，DIP浓度平均值为0.18μmol/L，N：P为11.2：1，硅酸盐浓度平均值为4.23μmol/L，表层叶绿素a平均值1.45 mg·m~(-3)，表层同化指数平均为4.12mg/，初级生产力平均值276.60 mgC/(m~2·d)，根据初级生产力计算潜在渔业资源碳生产量平均为0.34 gC/(m~2·a)，该区主要受南部湾口区海流向湾内延伸，沿岸海南岛河流注入湾内和琼州海峡过道水的影响，适合作为渔业作业区和水产养殖区；湾中部和南部远岸深水区，该区的环境主要特点是平均水深75m，DIN浓度平均值为0.77μmol/L，DIP浓度平均值为0.15μmol/L，N：P为5.1：1，硅酸盐浓度平均值为3.05μmol/L，表层叶绿素a平均值0.70 mg·m~(-3)，表层同化指数平均为3.69 mg/，初级生产力平均值350.89 mgC/(m~2·d)，根据初级生产力计算潜在渔业资源碳生产量平均为0.43 gC/(m~2·a)，该区主要受北部湾环流影响，适合作为渔业作业区。

A boiler water controlling system with basifier a mg/L Na3PO4+ b mg/L NH3+c mg/L NaOH is investigate in the paper.Several calculate methods are discussed and the precision pH value calculating equation is proposed for a cycle chemistry system that has seven changing parameters coexisting together,i.e.a mg/L Na3PO4+b mg/L NH3+c mg/L NaOH+d mg/L H2SO4+e mg/L CH3COOH+f mg/L CO2+g mg/L SiO2.As an ...

对于一个采用amg/L Na3PO4+bmg/L NH3+cmg/L NaOH碱化剂的炉水控制体系，主要讨论在炉水中存在dmg/L H2SO4+emg/L CH3COOH+fmg/L CO2+gmg/L SiO24种杂质时炉水pH值的计算方法，得到通用的pH值计算表达式；并以炉水平衡磷酸盐处理工况为例，计算这4种杂质在0-1 mg/L变化时，其单独或联合作用对炉水pH值的具体影响程度，以6幅图形展示计算结果，该结果可应用于炉水pH值的实际控制。

These uncontaminated explants showed that morphogenetic potential were 70%-75% and each responding explant had 1.2-1.4 shoots formation on MS medium supplemented with 2 mg/L NAA and 16 mg/L BA. Moreover, adding antibiotics (100 mg/L Rifampicin and 100 mg/L Gentamycin) to solidify MS contained 3% galactose were tested, results showed the lowest contamination rates were 20% and 1.0-1.2 shoots were regenerated per responding explant. The antibiotic treatments with 100 mg/L Rifampicin and 100 mg/L Gentamycin could reduce internal contaminants, while the addition of antibiotics into medium in vitro culture led to phototoxic phenomena of axillary explants, morphogenetic potential lost and the growth of regenerant was also inhibited.

以100 mg/L Rifampicin+100 mg/L Gentamycin或100 mg/L Rifampicin+100 mg/L Gentamycin+150 mg/L Streptomycin组合浓度处理者，具有显著降低污染率的效果分别为60%及50%；克服内生菌污染而建立初代无菌培养之腋芽培植体，则以100 mg/L Rifampicin或以100 mg/L Rifampicin+100 mg/L Gentamycin处理者，芽体再生率最高70%-75%，惟平均每一腋芽培植体芽体增殖数仅1.2-1.4个；另在醣种类及浓度与抗生素试验，显示100 mg/L Rifampicin+100 mg/L Gentamycin之抗生素浓度组合添加至含3g/L半乳糖的培养基，显著降低污染率至20%；但未污染之培植体，形态发生潜能低至40%以内，可反应生长之培植体芽体形成数也仅达1-1.2个之间，再生芽体之生长也显现抑制现象。

Results MRS medium was more suitable for producing bacteriocin than other media. The maximum plantaricin production was obtained in modified MRS medium containing 10 g/L maltose and 10 g/L glucose, 10 g/L yeast extract, 10 g/L tryptone and 2g/L tri-ammonium citrate, 1 mL/L Tween80, 6 g/L K2HPO4 ?

最佳培养基为MRS培养基；优化后的培养基配方为葡萄糖10 g/L，麦芽糖10 g/L，酵母提取物10 g/L，蛋白胨10 g/L，柠檬酸三铵2 g/L，吐温80为1 mL/L，K2HPO4·3H2O 6 g/L，乙酸钠5 g/L，硫酸镁0.2 g/L，硫酸锰

The results showed that the optimal auto-induction medium is: tryptone 19.17 g/L, yeast extract 9.59 g/L, Na2HPO4 5.72 g/L, KH2PO4 5.48 g/L,(NH4)2SO4 2.66 g/L, NaCl 3.33 g/L, glycerol 2%, glucose 0.68 g/L, lactose 6.33 g/L, MgSO4

实验结果表明，最优培养基成分为蛋白胨19.17 g/L，酵母膏9.59 g/L， Na2HPO4 5.72 g/L， KH2PO4 5.48 g/L，(NH4)2SO4 2.66 g/L， NaCl 3.33 g/L，甘油2%，葡萄糖0.68 g/L，乳糖6.33 g/L， MgSO4

At the same time ,the cap of filter paper was used for improving transformation ratio. Leaf discs were transferred onto the media MS+ NAA(1mg/L)+6-BA(3mg/L)+Km(30mg/L)for regeneration resistant selction. Shoots from selection medium were cultured on medium MS +NAA(1mg/L)+6-BA(3mg/L)+Km(50mg/L) for selection again,and then on the media 1/2MS+NAA(0.2mg/L)+IBA(0.2mg/L)+Km(25mg/L)for rooting selectin.

共培养后把叶盘转入MS+ NAA(1mg/L)+6-BA(3mg/L)+Km(30mg/L)培养基上进行分化筛选培养。30天后可分化出不定芽，把不定芽转到MS +NAA(1mg/L)+6-BA(3mg/L)+Km(50mg/L)培养基上进行抗性芽的筛选，转到1/2MS+NAA(0.2mg/L)+IBA(0.2mg/L)+Km(25mg/L)培养基上进行生根筛选。

L~(-1) each, 2μL of upstream primer10nmol ? L~(-1, 3.5μL of Mg~(2+)25 mmol ? L~(-1,10μL of double-distilled water.

包括RT-Mixmre 20μL，10×PCRbuffer 5μL，Taq5U·μL~(-11μL，dNTPs混合物2.5mmol·L~(-1each5μL，上游引物10μmol·L~(-12μL，Mg~(2+)25mmol·L~(-17μL，水10μL。

Nine different aphid-resistant alfalfas and four cross bred F1 were used in this experiment to investigate better dosage in SSR technique system and to seek the best combination among factors.At last,the best SSR technique system was established. The Taq DNA polymerase was 0.2μL(5U/μL),the 10×Buffer(Mg2+Plus)was 2.5μL , the dNTP Mixture was 2μL ( 2.5mmol/L), the primer was 1.5μL (10μmol/L),the template DNA was 1μL,the ddH2O was 16.3μL ,and the whole system was 25μL.

以九个不同抗蚜级别的苜蓿品种及四个杂交F1代为试材，摸索SSR反应体系中各个影响因素的浓度和用量，确定适合苜蓿抗蚜虫基因定位的SSR反应体系：其中Taq DNA聚合酶0.2μL(5U/μL)，10×Buffer(Mg2+Plus) 2.5μL，dNTP Mixture 2μL (各2.5mM)，引物各1.5μL (10μM)，模板DNA1μL，ddH2O 16.3μL补足25μL。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了