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The optimal carbon source and nitrogen source were also determined as 20 g/L glucose, 15 g/L tryptone and 5 g/L beef extract, respectively.

经过对产酶条件的优化，确定最佳摇瓶产酶条件为：葡萄糖20 g/L，蛋白胨15 g/L，牛肉膏5 g/L，玉米浆15 g/L， KH2PO4 3 g/L， MgSO4·7H2O 0.5 g/L，初始pH 7.0，培养温度37℃，装液量20 mL/250 mL三角瓶，摇床转速200 r/min。

RESULTS The average of total cholesterol,famish blood glucose,triglyceridein male were 4.62 mmol/L,4.37 mmol/L,1.68 mmol/L,respectively.

结果男性胆固醇、血糖、甘油三酯均值分别为4.62mmol/L、4.37mmol/L和1.68mmol/L，女性胆固醇、血糖、甘油三酯均值分别为4.99mmol/L、4.57mmol/L和1.61mmol/L。

It was known that pyridine and Triton-x100 had a bad effect on the cell growth and lycopene production were studied. The results indicated that pyridine and triton-x100 inhibited cell growth and lycopene synthesis;β-ionone increased cell growth and lycopene synthesis when the concentration was 0.05 ml/L, but when the concentration became higher, there would be a decrease on the lycopene output; span-20 increased cell growth and lycopene synthesis effectively when the concentration reached 2.0ml/L, and the lycopene yield became 2.4×10~(-2) mg/L which was 3-fold than control.

研究了培养基中加入不同浓度的小分子效应物吡啶、Triton-x100、Span-20、β-紫罗酮、异烟肼对番茄红素产量的提高作用，结果显示培养基中加入很低浓度的吡啶(0.1ml／L)就会对菌体产生很强的毒害作用；triton-x100对类胡萝卜素的合成也有很强的抑制作用，试验的浓度为0.2ml／L到5ml／L，结果发现它对菌体的生长和番茄红素的合成都有抑制作用；β-紫罗酮在浓度为0.05ml／L时有很小的促进作用，而浓度升高就有了比较强的抑制效果；span-20在液体培养基中的加量为2.0ml／L时，对番茄红素的合成有很强的促进效果，产量达到了2.4×10~(-2)mg／L，比原始菌株产量提高了2倍，而浓度进一步增大就产生了很强的抑制效果。

Five nitrogen concentrations including 0 mg/L,150 mg/L,300 mg/L,450 mg/L and 600 mg/L were sprayed on the root of D.styracifolium in field to study the effects of different N concentration on gas exchange,chlorophyll content,soluble protein content and nitrate reductase activity in leaves of seedlings.

采用田间试验的方式，设0mg/L，150mg/L，300mg/L，450mg/L和600mg/L5个N浓度处理，研究其对广金钱草苗期叶片气体交换、叶绿素含量、可溶性蛋白含量和硝酸还原酶活性的影响。

Result The results indicated that the optimal medium of shoot inducement for leaf-explant was MS+6-BA 1.00 mg/L+NAA 0.50 mg/L, and the optimal concentration of Kanamycin and Cefotaxime sodium in gene-engineering was 40 and 300 mg/L, respectively. The rootage rate of the reborn bud might reach 82% in MS medium with IBA 0.10 mg/L.

结果］结果表明，三倍体毛白杨最适合的不定芽分化培养基配方为MS+6-BA 1.00 mg/L+NAA 0.50 mg/L；在基因工程中，40 mg/L的卡那霉素为选择性抗生素使用浓度，300 mg/L的头孢霉素为抑菌性抗生素使用浓度；再生的不定芽在MS+IBA 0.10 mg/L固体培养基中生根率可达82%。

In this study, the cDNA fragments of G-L intergenic region and polymerase activity module in L gene of two street rabies virus were cloned and sequenced. The nucleotides sequence of these cDNA fragments mentioned above and their deduced amino acid sequence were compared with that of rabies virus strains, rabies-related virus strains and other Rliabdoviridae published previously. The result show that the homology of nucleotide sequence of G-L IGR among MRV, DRV is 83.3%, compared with other rabies-related virus strains, the homology of G-L IGR is between 58.3%-91.7%. the length of G-L IGR of rabies virus is different in Rhabdoviridae.

本实验对狂犬病病毒野毒株MRV、DRV的G-L间隔区以及L基因聚合酶活性部位进行了克隆和测序，将所测定的结果和推定的氨基酸序列与国内外公开发表的狂犬病病毒其它固定毒株、野毒株和同科不同属的病毒的相应部分进行了分析比较，结果表明MRV、DRV G-L IGR核苷酸序列的同源性为83.3％，与其它毒株的同源性在58.3％～91.7％之间；狂犬病毒G-L间隔区序列的长度在弹状病毒科不同病毒属之间有所不同。

Results: Good linearity was observed in 6～1512μmol.L-1 range for phenylalanine, the intra-run and inter-run imprecisions w ere 3.68%and 4.01%, the recovery rate was 98.6%; good linearity was observed in 5.4 ～1380.0μmol.L-1 range for tyrosine, the intra-run and inter-run i mp recisions were 1.87%and 2.59%, the average recovery rate was 98.9%.

酪氨酸的线性范围为5.4～1380μmol.L-1，最低检测浓度为0.7。μmol* L-1，平均回收率98.9%，批内CV为1.87%，批间CV为2.59%。健康儿童血清苯丙氨酸浓度为(61.4±10.8)μmol.L-1，血清酪氨酸浓度为(131.8±28.0)μmol.L -1。健康成人血清苯丙氨酸浓度为(69.3±8.6)μmol.L-1，血清酪氨酸浓度为(83.6±9.4)μmol.L-1.4例苯丙酮尿症患儿的血清苯丙氨酸浓度为1296～1759μmol.L-1，血清酪氨酸为29.5～53.5μmol.L-1。

Results showed that there were significantly positive linear correlations between the toxic effects（probits of mortality and inhibition rate） of chlorpromazine on the two aquatic organisms and the concentrations of chlorpromazine, and the toxic intensity became higher as the exposure time increased. The LC50 values of chlorpromazine acting on Carassius auratus in 24, 48 and 96h were 1.11, 0.43 and 0.32mg·L-1, respectively; and its safe concentration for Carassius auratus was 19.5μg·L-1. The LC50 and EC50 （50% of the tested Daphnia magna inhibited） values of chlorpromazine acting on Daphnia magna in 24h were 0.65 and 0.57mg·L-1, and the LC50 and EC50 values in 48h were 0.36 and 0.28mg·L-1, respectively. Thus, chlorpromazine have high toxicity to Carassius auratus and Daphnia magna, and the latter is more sensitive to chlorpromazine.

结果表明：氯丙嗪对两种水生生物的毒性效应（致死率、抑制率概率单位）均与其浓度呈显著线性正相关关系（p.05），且毒性强度随作用时间的延长而增加；氯丙嗪对鲫鱼的24、48和96h的半致死浓度（LC50）值分别为1.11、0.43和0.32mg·L-1，通过计算求得氯丙嗪对鲫鱼的安全浓度为19.5μg·L-1；氯丙嗪对大型蚤的24h LC50值为0.65mg·L-1，24h EC50值（使50%受试大型蚤活动受抑浓度）为0.57mg·L-1；48h LC50值为0.36mg·L-1， 48h EC50值为0.28mg·L-1；对鲫鱼和大型蚤而言，氯丙嗪属极高毒性物质，大型蚤对氯丙嗪的敏感性大于鲫鱼。

Methods ①The spiral organ of corti of 10 mice (20 ears) were taken, and divided into 2 groups randomly (n=10), cultured in 0.1 mol·L-1 gentamicin and the metabolite of 0.1 mol·L-1 gentamicin for 48h, then the number of hair cells remain was recorded;②The spiral organ of corti of 10 mice were taken (20 ears), and divided into 2 groups randomly (n=10), 3% CaCl2 and 0.1 mol·L-1 GM were mixed in the culture medium and the number of hair cells remain in the two medium mentioned above were recorded;③The spiral organ of corti of 10 mice were taken (20 ears), divided into 2 groups randomly (n=10), 3% MgSO4 to 0.1 mol·L-1 GM were mixed in the culture medium, the number of hair cells remain in the two medium mentioned above were recorded.

①取10只小鼠的耳蜗螺旋器（20个），随机分为2组（n=10），分别在含0.1 mol·L-1 GM与0.1 mol·L-1GM代谢产物的培养液中培养48 h后，记数残留的耳蜗毛细胞数；②取10只小鼠的耳蜗螺旋器（20个），随机分为2组（n=10），分别在含0.1 mol·L-1 GM与0.1 mol·L-1GM+3% CaCl2的培养液中培养48 h后，记数残留的耳蜗毛细胞数；③取10只小鼠的耳蜗螺旋器（20个），随机分为2组（n=10），分别在含0.1 mol·L-1 GM与0.1 mol·L-1GM+3% MgSO4的培养液中培养48 h后，记数残留的耳蜗毛细胞数。

The optimal medium components were: sodium alginate 3 g/L,(NH4)2SO4 3 g/L, NaCl 20 g/L, KH2PO4 0.1 g/L, CaCl2 0.1 g/L; The optimal culture conditions were: 25 ml medium in 250 mL Erlenmeyer flask, inoculum's volume 3%, shaking speed of 150 r/min, initial pH 7.5, at 28°C for 24 h.

该菌株产酶的最佳培养基组成为：褐藻胶3 g/L、(NH4)2SO4 3 g/L、NaCl 20 g/L、KH2PO4 0.1 g/L、CaCl2 0.1 g/L；最佳培养条件为：250 mL三角烧瓶中装液量25 mL、接种量3%、摇瓶转速150 r/min、pH7.5、培养温度为28℃、培养时间为24 h。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了