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Tamarense was studied. 5 mg·L~-1 of N-phenyl-2-naphthylamine was showed to have 50% inhibitory rate on A. tamarense after 3 days. 70 μL·L~-1 of linoleic acid appeared 40% inhibition rate on A. tamarense.70 μL·L~-1 of pelargonic acid had 85% inhibition rate at the 3 rd day with some increase in cell density later on.

结果表明，浓度高于5 mg·L-1以上的N-苯基-2-萘胺，3 d后能保持对塔玛亚历山大藻抑制率超过50%；浓度为70μL·L-1的亚油酸对塔玛亚历山大藻的抑制率约为40%；浓度为70 μL·L-1的壬酸第3天可以达到85%的抑藻率，但随后藻密度有反弹。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

The results indicates, with the same level of other parameters, the TN removal can reach 80.0% and 70.9% with the control of dissolved oxygen under the levels of 0.3-0.6mg/l and 0.6-0.9mg/l, respectively; the TN removal upgrades from 80%, 83% to 84.1% with the decrease of sludge load in the system from 0.1, 0.07, to 0.05gCOD/gMLSS·d; the nitrogen removal only reaches 58% when C/N is 4, but can reach more than 80% when C/N is at or over 7, which indicates that the TN removal increases when higher C/N ratio; when HRT increases from 12h, 18h to 24h, the TN removal also increases from 78.6%, 82.0% to 83.0%; the TN removal average in the system increases from 75.6%, 78.5% to 83.0% with the increase of sludge age from 18d, 20d to 24d; the low oxygen conditions in the modified Orbal oxidation ditch model do not decrease the effect of COD removal, and the dissolved oxygen gradient with the distribution of 0-2-1mg/l in the system ensures the COD removal reaching the range of 80%-86%; and the phosphate removal, however, is not good enough, which varies from 40% to 50%, even lower than 35% in occasional situations, and the phosphate concentration in the outflow varies in the range of 1.28-2.38mg/l, which dissatisfies the discharge standard with the level of 1.0mg/l.

试验结果表明，在其它参数相同的情况下，将外沟道的溶解氧分别控制在0.3～0.6mg/l 和0.6～0.9mg/l 两个水平，外沟道中TN 去除率分别为80.0%和70.9%；系统中污泥负荷分别为0.1gCOD/gMLSS·d、0.07 gCOD/gMLSS·d、0.05 gCOD/gMLSS·d时，随着污泥负荷的降低，TN 的去除率却是上升的，分别为80%、83%、84.1%；本试验中，当C/N 为4时，仅能获得58%的脱氮率，当C/N 为7 或更高时脱氮率能达到80%以上，进水C/N 比值越高，总氮的去除率越高；在本试验中采取的水力停留分别为12h、18h、24h 时，外沟道中的总氮去除率随水力停留时间的增加而增加，分别为78.6%、82.0%和83.0%；当系统泥龄分别为18d、20d、24d 时，系统平均TN 去除率分别为75.6%、78.5%、83.0%，随着泥龄的增大TN 的去除率增加；改良型奥贝尔模型的低氧条件并没有降低COD 的去除效果，系统0-2-1mg/l的溶解氧梯度分布使得COD 去除率一般都在80%～86%之间；本试验中磷的去除效果不好，去除率基本在40%～50%之间，有的甚至低于35%，出水磷的浓度在1.28～2.38mg/l 之间，低于1.0mg/l 的出水排放标准。

In this study,the muscarinic current was induced by carbachol(50 umol/ L) or GTPYB(0.5 mmol/L).The results showed that hyposmotic superfusate(202mOsmol/ L) increased carbachol-induced current by 145士27%and increased GTPYS-induced current by 183士30%;but in the presence of cytochalasirr B(cyt- B,20 umol/ L),an actin cytoskeleton disrupter,posmotic membrane stretch increased 1CC6 by 70士6%.However,hypos motic membrane tretch induced increase in 1CC6 was potentiated to 545士81% by phalloidin(20 umol/ L),an actin microfilament stabilizer.

当豚鼠胃窦平滑肌细胞的膜电位钳制在一20 m V时，灌流液中50umol/ L卡巴胆碱(carbachol ， CCh)或电极内液中0.5 mmol/L GTPYS均可引导毒覃碱电流，2 m Os mol/ L分别使其增加145士27%和183士30；当电极内液中加入20 umol/ L的细胞松弛B时，低渗牵张使1 cce只增加70士6；而电极内液中加入20 umol/ L的鬼笔环肤则使Icce增加了545士81。

It is found that the best refolding yield for 0.1g/L linear HNTX-Ⅲ could be obtained in double-distilled aqueous solution at pH 7.5 containing 1.0 mol/L L-arginine, 1.0mmol/L reduced glutathione, and 0.1mmol/L oxidized glutathione.

最佳的氧化复性条件为pH 7.5的重蒸水溶液体系，样品质量浓度为0.1mmol/L，还原型谷胱甘肽和氧化型谷胱甘肽的浓度分别为1.0mmol/L和0.1mmol/L、L-Arg浓度为1.0mol/L。

METHODS: The bile was treated with 3 different methods, including the 2D-Clean-up kit group: the bile sample was dissolved with lysis buffer (contains: 7 mol/L carbamide, 2 mol/L thiourea, ammonium persulfate with mass concentration of 0.4 g/L, 65 mmol/L dithiothreitol, carrier ampholytes with mass concentration of 0.2 g/L).

将标本分别按3种不同方法进行处理：①2D-Clean-up试剂盒处理胆汁标本：用裂解液，7 mol/L尿素，2 mol/L硫脲，质量浓度为0.4 g/L的过硫酸铵，65 mmol/L二硫苏糖醇，质量浓度为0.2 g/L的载体两性电解质溶解沉淀过夜。

METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

采用全骨髓法体外分离培养人骨髓间充质干细胞，传至第3代以1×108 L-1密度接种至预置盖玻片的6孔培养板中，设立3组：成骨诱导组添加原代培养液后，再加入含10-8 mol/L地塞米松、10 mmol/L β-磷酸甘油、50 mg/L维生素C的成骨诱导培养基；成骨+瘦素诱导组添加成骨诱导培养基后，再加入50 nmol/L瘦素；空白对照组仅加入含体积分数为10%胎牛血清的LG-DMEM培养液。

ES cells were maintained on gelatin-coated dishes in Iscove's modified Dulbecco medium containing 0.1 mmol/L of nonessential amino acids, 2 mmol/L of L-glutamine, 0.1 mmol/L of monothioglycerol, 15% fetal bovine serum, and 1000 U/mL leukemia inhibitory factor. Embryonic bodies were firstly formed by ES cells with hang drip method in IMDM without LIF. Six-day-old EBs were trypsinized and the cells were resuspended at a concentration of （3-4）×109 L-1 cells per 1 mL of a 1:1 mixture of culture medium and Matrigel?

实验方法：将鼠胚胎干细胞株接种于被覆明胶的培养瓶内，加入含0.1 mmol/L非必需氨基酸、2 mmol/L L-谷酰胺、0.1 mmol/L二羟基丙硫醇、15%胎牛血清、1 000 U/mL白血病抑制因子的IMDM培养液。3 d后采用&悬滴法&使细胞在不含白血病抑制因子的上述IMDM培养液中形成胚胎体，6 d后胰酶消化得到胚胎干细胞，浓度调整为（3~4）×109 L-1，与Matrigel？

Separated cultures were isolated from 4 different koumiss examples, the complete 16SrRNA gene of these strains were sequenced, and phylogenetic trees were constructed. The isolateswere identified to the most related strain which had the highest affinity. The identified strains weresix L. lactis, five L. plantarum, five E. faecium, four E. faecalis, two L. casei, two L. helveticus, twoS. thermophilus, one L. delbrueckii subsp, bulgaricus, one Leu. mesenteroides, one Leu. garlicum.

本研究中从4份酸马奶中分离得到了29株乳酸菌，测定了这些乳酸菌的16S rRNA基因全长序列，并构建了乳酸菌属的系统发育树，根据亲缘关系对乳酸菌进行了鉴定，其中L.lactis(6株)，L.plantarum(5株)，E.faecium(5株)，E.faecalis(4株)，L.casei(2株)，L.helveticus(2株)，S.thermophilus(2株)，L.delbrueckii subsp.bulgaricus(1株)，Leu.mesenteroides(1株)，Leu.garlicum(1株)。

Bath application of chloride channel blocke(rNPPB30μmol/L)and potassium channel blockers(TEA10mmol/L and 4-AP10mmol/L) could almost completely block the outward current, only remaining 2.1±0.2%.Bath application of Ca2+-free Ringer's solution and potassium channel blockers(TEA10mmol/L and 4-AP10mmol/L) could almost completely block the outward current, only remaining 3.2±0.3%.

用氯离子通道拮抗剂NPPB(30μmol/L)与钾离子通道拮抗剂(TEA10mmol/L和4-AP10mmol/L)协同作用时，该电流也可被基本抑制，只剩2.1±0.2%；(3)用无钙Ringer氏液与钾离子通道拮抗剂(TEA10mmol/L和4-AP10mmol/L)协同作用时，可将此电流基本抑制，仅剩3.2±0.3%。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了