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And then adding NAA at low concentration in MS was used to give rise to roots. We also found that lower level of hormone could control effectively browninng and vitrifaction during the culture and G1 n (6mg/L) and AgNOj (2mg/L) supplemented in shoot induction media could improve the shoot information rates apparently (about90%).The whole period of plant regeneration from leaflets of peanut could be divided as five steps: germination - shoot induction -shoot elongation-rooting- tranplant.

用1／2MS培养基萌发花生种子，9-10d后，从无菌花生苗上切取幼嫩叶片中部为外植体。2500Lux光照和27±1℃条件下，在诱芽培养基(MS+BA3mg／L+NAA0.8mg／L+AgNO_32mg／L十Gln 6mg／L)培养12-14d即可观察到明显芽点或瘤状突起，较前人报道的培养时间大大缩短了，4w后芽点进一步发育成丛生芽，芽诱导率达90.2％，每个外植体平均产9个丛尘芽，然后转至培养基MS+BA3 mg／L+AgNO_32 mg／L上诱导芽的伸长，3-4w后可长至3-4cm，切下带有2-3片叶片的幼芽移至生根培养基(MS+NAA0.8mg／L+AgNO_32mg／L)，1w后切口处可见白色不定根形成。

Methods; Rat HSCs were treated with 0.4% fetal calf serum/DMEM for 48 hours and divided into the control group and experiment groups. HSCs in the control group were treated with 10% FCS, and HSCs in the experiment groups were treated with 10% FCS and low anticoagulative activity heparin with different concentrations (8 g/L, 40 g/L, 200 g/L, 1000 g/L, 5000 g/L). MTT reduction assay was used to evaluate the effect of low anticoagulative activity heparin on the growth of rat HSCs.

肝星状细胞经体积分数为0.4%胎牛血清／DMEM培养液同步化48h后分为对照组和实验组，对照组以体积分数为10%胎牛血清处理，实验组分别以体积分数为10%胎牛血清和不同质量浓度(8 g/L， 40 g/L， 200 g/L， 1000 g/L， 5000 g/L)低抗凝肝素处理，应用MTT法测定其对肝星状细胞生长的影响。

An optimized PCR reaction system for ISSR analysis was established:PCR was performed in a 20μl reaction mixture with 5μmol/L DNA, 2.5mmol/L Mg~(2+), 0.3mmol/Lof each dNTP, 300nmol/L primer, 1U Taq polymerase and 1×Buffer. Using 9 ISSRprimers,the genetic diversity among 23 Arrhenatherum elatius L.

3遗传多样性研究结果表明：通过模板DNA、引物、Mg~+、dNTPs、Taq酶五种ISSR反应体系的影响因素进行L_(16)(4~5)正交试验，建立了高燕麦草ISSR反应体系：20μl的反应体积中含有模板DNA 5μmol／L，引物300nmol／L，Mg~+2.5mmol／L，dNTP 0.3mmol／L，Taq酶1U及1×Buffer。

In the study, embryo bud of Impatiens balsamina L was cultured on hormone-free MS medium for promoting, MS medium with 6-BA 3 mg/L+NAA 0.5 mg/L for shoot and root formation, and MS medium containing 6-BA 3 mg/L+NAA 0.5 mg/L for flowering respectively. During about 60 days, plant regeneration and flowering of Impatiens baisamina L was finished in vitro.

研究以凤仙花无菌苗胚芽段作为外植体，经过不含任何激素的MS(0)培养基预培养、MS+6-BA3mg/L+NAA0.5mg/L培养基的诱导芽和根分化、MS+6-BA1mg/L+NAA0.1mg/L培养基的诱导开花，在60d左右实现了凤仙花离体培养再生植株并在试管内开花。

Samples of the equilibrium solid phase in Rb_2SO_4-PEG4000-H_2O system at 25 ℃ analyzed by TGA showed that they consisted of anhydrous salt. Six zones were observed in the complete phase diagram. They are L, L+L, L+S, 2L+S and L+2S, respectively. The binodal curves were given using a four-parameter equation y = a + bx~(0.5)+cx+dx~2. The tie lines were correlated by the Othmer-Tobias and Bancroft equations.

同时我们用热重分析仪对Rb_2SO_4—PEG 4000—H_2O三元体系在25℃时的平衡固相进行分析，发现在实验研究的温度范围内，体系的平衡固相均为无水盐，且在体系的完整相图中，观察到六个相区，分别为L，L+L，L+S，2L+S和L+2S相区，即非饱和的单液相区，非饱和的双液相区，饱和的单液相区，饱和的双液相区和两种固相(Rb_2SO_4，PEG 4000)存在的饱和单液相区。

The results were showed that somatic embryos can be directly re-induced from cotyledonary, which was cultured on MS medium supplemented with ZTO.5-2 mg/L, BA 1mg/L and NAA 0.2mg/L, and the induction rate was over 80%.

结果表明，MS+ZT 0.5～2mg／L+BA 1mg／L+IAA 0.2mg／L培养基可直接诱导体胚发生，诱导率超过80％；MS+BA 2mg／L+NAA 0.2 mg／L或MS+TDZ 0.01 mg／L+NAA 0.2 mg／L培养基可直接分化不定芽，出芽诱导率分别为88％、100％。

The sludge settling characteristic is satisfying with SV varying from 22 to 30 and SVI varying from 58 to 80ml/g.The sludge bulking problem has been avoided successfully. The excess sludge can be thickened to 3%-4 %. The alkalinity remains equilibrious between the nitrification and denitrification in SBR-BAF system. The COD and TP loading of SBR are 2.08kgCOD/kgMLSS·d and 41.68gTP/kgMLSS·d, respectively. The NH4+-N loading of BAF is 1.16kgNH_4~+-N/m~3.d. The total HRT is about 10 hours which is only about half of suspended growth process for simultaneous phosphorous and nitrogen removal. Under the above mentioned high loadings and short HRT, the mean removal efficiency of COD, TP, NH4+-N, TN is 96%, 98%, 93%, and 84%, respectively. The mean effluent concentration of COD, TP, NH4+-N, TN, SS is 20mg/L, 0.23 mg/L, 3.24 mg/L, 7.68 mg/L, 5 mg/L respectively, which means every index can meet the first class of Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant.

最后，以上述最佳运行参数控制SBR-BAF系统运行，长期运行结果表明：系统中悬浮污泥的絮凝、沉降性能良好，SV值为22~30，SVI值为58~80，不再有污泥膨胀之虞，而且可以对剩余污泥进行重力浓缩，浓缩后污泥浓度可以达到3~4%；短程硝化和反硝化使得系统可以维持碱度平衡，无需额外补充碱度；系统在高负荷(SBR反应器COD负荷为2.08kgCOD/kgMLSS.d，TP负荷为41.68gTP/kgMLSS.d，BAF反应器NH4+-N负荷为1.16kgNH_4~+-N/m~3.d)、低水力停留时间(SBR反应器9h，BAF反应器1h，总计10h，约为同步脱氮除磷悬浮污泥工艺的一半)下稳定高效运行，对COD(不计BAF加入的外碳源)、TP、NH_4~+-N、TN的平均去除率为96%、98%、93%、84%，出水COD、TP、NH4+-N、TN、SS的平均浓度为20mg/L、0.23 mg/L、3.24 mg/L、7.68 mg/L、5 mg/L，各项水质指标均达到《城镇污水处理厂污染物排放标准》(GB 18918-2002 )一级标准的A标准的要求。

In the room under the normal temperature still water condition, uses the trichloro fulminuric acid to fork the tail to the spot 鮰 to carry on the acute toxicity test, finally demonstrated: 24h, 48h, 72h, the 96h half lethal concentration respectively is 9.6mg/L, 8.7 mg/L, 7.2mg/L, 6.2 mg/L, in the safe concentration 0.62 mg/L.

在室内常温静水条件下，采用三氯异氰尿酸对斑点叉尾鮰进行急性毒性试验，结果显示：24h、48h、72h、96h半致死浓度分别为9.6mg/L、8.7 mg/L、7.2mg/L、6.2 mg/L，安全浓度0.62 mg/L。

Good linearities were obtained for nine organic acids, and the detection limits were 0.5 mg/L, 0.3 mg/L, 1.5 mg/L, 1.5 mg/L, 0.3 mg/L, 0.3 mg/L, 0.4 mg/L, 0.4 mg/L, 0.4 mg/L for malonic, formic, tartaric, malic, succinic, glutaric, acetic, lactic and glutamic acid, respectively.

cm为分离通道，在6 min内实现了9种酸的完全分离。9种有机酸的浓度与其峰面积在一定的范围呈良好的线性关系，检出限均低于1.5 mg/L，迁移时间和峰面积的日内、日间相对标准偏差均小于6%。

The culture ingredients and conditions were optimized with the orthonormal table L_9(3~4) method. Studies showed that the culture components for optimization of β-mannanase production were yeast extract 20 g/L, Konjak powder 20 g/L,(NH_4)_2SO_4 2 g/L, KH_2PO_4 1 g/L and MgSO_4 · 7H_2O 1 g/L; and the optimal culture conditions were seed incubation time 8-12 h, inoculation ratio 6%, culture volume 25 mL, pH 7.5, fermentation temperature 40 ℃ and fermentation time 25 h.

通过正交设计方法筛选优化该菌培养基配方和产酶条件，培养基配方为：酵母抽提物20g／L、魔芋粉20 g／L、(NH_4)_2 SO_4 2 g／L、KH_2PO_4 1 g／L、MgSO_4·7H_2O 1 g／L；产酶条件为：菌种种龄8～12h、接种量6％、装液量25 ml、pH7.5、培养温度40℃、培养时间25h。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。